Restriction map for
pF1KB4495
(Linear) (Six-base) MAPSORT of: KIBB4495.seq Check: 2856 from: 1 to: 2889
KIBB4495.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 822 2889
Size: 822 2067
AccI GT'mk_AC
Cuts at: 0 143 2889
Size: 143 2746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 699 1711 2434 2506 2841 2889
Size: 241 458 1012 723 72 335 48
Fragments arranged by size:
1012 723 458 335 241 72 48
AfeI AGC'GCT
Cuts at: 0 727 2566 2791 2889
Size: 727 1839 225 98
Fragments arranged by size:
1839 727 225 98
AleI CACnn'nnGTG
Cuts at: 0 2212 2889
Size: 2212 677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1036 1511 2230 2889
Size: 1036 475 719 659
Fragments arranged by size:
1036 719 659 475
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 737 769 2889
Size: 737 32 2120
Fragments arranged by size:
2120 737 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1112 1245 2232 2303 2505 2748 2889
Size: 1112 133 987 71 202 243 141
Fragments arranged by size:
1112 987 243 202 141 133 71
ApaI G_GGCC'C
Cuts at: 0 2175 2197 2889
Size: 2175 22 692
Fragments arranged by size:
2175 692 22
ApaLI G'TGCA_C
Cuts at: 0 2140 2889
Size: 2140 749
ApoI r'AATT_y
Cuts at: 0 2245 2889
Size: 2245 644
AvaI C'yCGr_G
Cuts at: 0 424 820 1024 2190 2197 2383 2889
Size: 424 396 204 1166 7 186 506
Fragments arranged by size:
1166 506 424 396 204 186 7
BamHI G'GATC_C
Cuts at: 0 14 2889
Size: 14 2875
BanI G'GyrC_C
Cuts at: 0 1536 2889
Size: 1536 1353
BanII G_rGCy'C
Cuts at: 0 1097 1340 1751 2175 2197 2384 2889
Size: 1097 243 411 424 22 187 505
Fragments arranged by size:
1097 505 424 411 243 187 22
BbvCI CC'TCA_GC
Cuts at: 0 1826 2889
Size: 1826 1063
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 630 664 2889
Size: 630 34 2225
Fragments arranged by size:
2225 630 34
BglI GCCn_nnn'nGGC
Cuts at: 0 696 1555 1860 2889
Size: 696 859 305 1029
Fragments arranged by size:
1029 859 696 305
BglII A'GATC_T
Cuts at: 0 1058 2889
Size: 1058 1831
BlpI GC'TnA_GC
Cuts at: 0 8 618 1144 2056 2203 2889
Size: 8 610 526 912 147 686
Fragments arranged by size:
912 686 610 526 147 8
Bme1580I G_kGCm'C
Cuts at: 0 527 1025 1500 2144 2175 2197 2889
Size: 527 498 475 644 31 22 692
Fragments arranged by size:
692 644 527 498 475 31 22
BmgBI CAC'GTC
Cuts at: 0 1035 2889
Size: 1035 1854
BmrI ACTGGGnnnn_n'
Cuts at: 0 122 1800 2816 2889
Size: 122 1678 1016 73
Fragments arranged by size:
1678 1016 122 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1111 1153 1174 1222 1354 2044 2242
Size: 436 675 42 21 48 132 690 198
Cuts at: 2242 2724 2889
Size: 482 165
Fragments arranged by size:
690 675 482 436 198 165 132 48
42 21
Bpu10I CC'TnA_GC
Cuts at: 0 76 1826 2889
Size: 76 1750 1063
Fragments arranged by size:
1750 1063 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1594 1861 1951 1969 2889
Size: 1594 267 90 18 920
Fragments arranged by size:
1594 920 267 90 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 1042 1435 2604 2889
Size: 1042 393 1169 285
Fragments arranged by size:
1169 1042 393 285
BsaAI yAC'GTr
Cuts at: 0 1020 1350 2255 2334 2889
Size: 1020 330 905 79 555
Fragments arranged by size:
1020 905 555 330 79
BsaBI GATnn'nnATC
Cuts at: 0 184 2889
Size: 184 2705
BsaHI Gr'CG_yC
Cuts at: 0 2321 2395 2618 2889
Size: 2321 74 223 271
Fragments arranged by size:
2321 271 223 74
BsaWI w'CCGG_w
Cuts at: 0 341 2889
Size: 341 2548
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 298 328 2889
Size: 298 30 2561
Fragments arranged by size:
2561 298 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 306 1263 1814 2077 2889
Size: 306 957 551 263 812
Fragments arranged by size:
957 812 551 306 263
BseYI C'CCAG_C
Cuts at: 0 535 1052 1695 2179 2561 2889
Size: 535 517 643 484 382 328
Fragments arranged by size:
643 535 517 484 382 328
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 1309 2734 2889
Size: 346 963 1425 155
Fragments arranged by size:
1425 963 346 155
BsiHKAI G_wGCw'C
Cuts at: 0 1097 1751 2144 2191 2209 2537 2889
Size: 1097 654 393 47 18 328 352
Fragments arranged by size:
1097 654 393 352 328 47 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 88 2889
Size: 88 2801
Bsp1286I G_dGCh'C
Cuts at: 0 527 1025 1097 1340 1500 1751 2144 2175
Size: 527 498 72 243 160 251 393 31
Cuts at: 2175 2191 2197 2209 2384 2537 2889
Size: 16 6 12 175 153 352
Fragments arranged by size:
527 498 393 352 251 243 175 160
153 72 31 16 12 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 580 822 2027 2348 2889
Size: 580 242 1205 321 541
Fragments arranged by size:
1205 580 541 321 242
BsrBI CCG'CTC
Cuts at: 0 22 1287 2067 2346 2388 2889
Size: 22 1265 780 279 42 501
Fragments arranged by size:
1265 780 501 279 42 22
BsrFI r'CCGG_y
Cuts at: 0 690 799 2889
Size: 690 109 2090
Fragments arranged by size:
2090 690 109
BsrGI T'GTAC_A
Cuts at: 0 464 2889
Size: 464 2425
BssSI C'ACGA_G
Cuts at: 0 2137 2889
Size: 2137 752
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2232 2889
Size: 2232 657
BstBI TT'CG_AA
Cuts at: 0 849 2889
Size: 849 2040
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 414 919 2889
Size: 300 114 505 1970
Fragments arranged by size:
1970 505 300 114
BstYI r'GATC_y
Cuts at: 0 14 1058 1605 2228 2889
Size: 14 1044 547 623 661
Fragments arranged by size:
1044 661 623 547 14
Bsu36I CC'TnA_GG
Cuts at: 0 1246 2889
Size: 1246 1643
BtgI C'CryG_G
Cuts at: 0 941 1365 2543 2889
Size: 941 424 1178 346
Fragments arranged by size:
1178 941 424 346
DraIII CAC_nnn'GTG
Cuts at: 0 358 1397 2889
Size: 358 1039 1492
Fragments arranged by size:
1492 1039 358
EaeI y'GGCC_r
Cuts at: 0 23 909 2591 2889
Size: 23 886 1682 298
Fragments arranged by size:
1682 886 298 23
EcoICRI GAG'CTC
Cuts at: 0 1095 1749 2889
Size: 1095 654 1140
Fragments arranged by size:
1140 1095 654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 436 699 1111 1153 1174 1222 1354
Size: 241 195 263 412 42 21 48 132
Cuts at: 1354 1711 2044 2242 2434 2506 2724 2841 2889
Size: 357 333 198 192 72 218 117 48
Fragments arranged by size:
412 357 333 263 241 218 198 195
192 132 117 72 48 48 42 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2271 2889
Size: 2271 618
EcoO109I rG'GnC_Cy
Cuts at: 0 62 1109 1598 2171 2194 2276 2502 2889
Size: 62 1047 489 573 23 82 226 387
Fragments arranged by size:
1047 573 489 387 226 82 62 23
HaeII r_GCGC'y
Cuts at: 0 729 2310 2568 2793 2889
Size: 729 1581 258 225 96
Fragments arranged by size:
1581 729 258 225 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 363 395 2889
Size: 363 32 2494
Fragments arranged by size:
2494 363 32
MscI TGG'CCA
Cuts at: 0 911 2593 2889
Size: 911 1682 296
Fragments arranged by size:
1682 911 296
MslI CAynn'nnrTG
Cuts at: 0 115 298 1018 1493 2212 2889
Size: 115 183 720 475 719 677
Fragments arranged by size:
720 719 677 475 183 115
MspA1I CmG'CkG
Cuts at: 0 12 365 1089 1298 1323 1566 1689 2088
Size: 12 353 724 209 25 243 123 399
Cuts at: 2088 2303 2889
Size: 215 586
Fragments arranged by size:
724 586 399 353 243 215 209 123
25 12
NcoI C'CATG_G
Cuts at: 0 1365 2889
Size: 1365 1524
NdeI CA'TA_TG
Cuts at: 0 1272 2889
Size: 1272 1617
PasI CC'CwG_GG
Cuts at: 0 1042 2325 2889
Size: 1042 1283 564
Fragments arranged by size:
1283 1042 564
PflMI CCAn_nnn'nTGG
Cuts at: 0 897 942 1397 2102 2505 2792 2889
Size: 897 45 455 705 403 287 97
Fragments arranged by size:
897 705 455 403 287 97 45
PfoI T'CCnGG_A
Cuts at: 0 1943 2522 2741 2889
Size: 1943 579 219 148
Fragments arranged by size:
1943 579 219 148
PmlI CAC'GTG
Cuts at: 0 1020 2889
Size: 1020 1869
PpuMI rG'GwC_Cy
Cuts at: 0 62 1109 2276 2889
Size: 62 1047 1167 613
Fragments arranged by size:
1167 1047 613 62
PspOMI G'GGCC_C
Cuts at: 0 2171 2193 2889
Size: 2171 22 696
Fragments arranged by size:
2171 696 22
PstI C_TGCA'G
Cuts at: 0 1117 1297 1565 2889
Size: 1117 180 268 1324
Fragments arranged by size:
1324 1117 268 180
PvuII CAG'CTG
Cuts at: 0 1298 1323 1566 1689 2088 2303 2889
Size: 1298 25 243 123 399 215 586
Fragments arranged by size:
1298 586 399 243 215 123 25
SacI G_AGCT'C
Cuts at: 0 1097 1751 2889
Size: 1097 654 1138
Fragments arranged by size:
1138 1097 654
SanDI GG'GwC_CC
Cuts at: 0 62 2889
Size: 62 2827
ScaI AGT'ACT
Cuts at: 0 564 2889
Size: 564 2325
SfcI C'TryA_G
Cuts at: 0 732 1113 1293 1561 2549 2889
Size: 732 381 180 268 988 340
Fragments arranged by size:
988 732 381 340 268 180
SmlI C'TyrA_G
Cuts at: 0 1609 1876 1966 1984 2889
Size: 1609 267 90 18 905
Fragments arranged by size:
1609 905 267 90 18
StyI C'CwwG_G
Cuts at: 0 230 407 1365 2096 2594 2889
Size: 230 177 958 731 498 295
Fragments arranged by size:
958 731 498 295 230 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1469 1554 1819 2889
Size: 1469 85 265 1070
Fragments arranged by size:
1469 1070 265 85
TatI w'GTAC_w
Cuts at: 0 464 562 2027 2889
Size: 464 98 1465 862
Fragments arranged by size:
1465 862 464 98
Tth111I GACn'n_nGTC
Cuts at: 0 1309 2889
Size: 1309 1580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 564 1039 2154 2610 2889
Size: 564 475 1115 456 279
Fragments arranged by size:
1115 564 475 456 279
XmnI GAAnn'nnTTC
Cuts at: 0 2455 2889
Size: 2455 434
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbvCI
BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I MscI MslI MspA1I NcoI NdeI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SanDI
ScaI SfcI SmlI StyI TaqII TatI Tth111I XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BciVI BclI
BfrBI BmtI BplI BsiEI BsiWI BsmI BspEI BspHI
BsrDI BssHII BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EarI EciI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XhoI XmaI ZraI