(Linear) (Six-base) MAPSORT of: KIBB4480.seq  Check: 5409  from: 1  to: 1077



KIBB4480.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AccI GT'mk_AC

Cuts at:      0     853    1077 
   Size:        853     224
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407    1077 
   Size:        407     670
 
AflII C'TTAA_G

Cuts at:      0     733    1077 
   Size:        733     344
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     747     779    1077 
   Size:        747      32     298

  Fragments arranged by size: 

                 747     298      32
 
ApoI r'AATT_y

Cuts at:      0     438     628     881    1006    1077 
   Size:        438     190     253     125      71

  Fragments arranged by size: 

                 438     253     190     125      71
 
AseI AT'TA_AT

Cuts at:      0     727    1077 
   Size:        727     350
 
BanI G'GyrC_C

Cuts at:      0     341    1077 
   Size:        341     736
 
BanII G_rGCy'C

Cuts at:      0    1044    1077 
   Size:       1044      33
 
BbeI G_GCGC'C

Cuts at:      0     345    1077 
   Size:        345     732
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     835    1077 
   Size:        835     242
 
BfrBI ATG'CAT

Cuts at:      0     724    1077 
   Size:        724     353
 
BsaAI yAC'GTr

Cuts at:      0     708    1077 
   Size:        708     369
 
BsaHI Gr'CG_yC

Cuts at:      0     342    1077 
   Size:        342     735
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     362     392    1077 
   Size:        362      30     685

  Fragments arranged by size: 

                 685     362      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     100     710    1052    1077 
   Size:        100     610     342      25

  Fragments arranged by size: 

                 610     342     100      25
 
BseYI C'CCAG_C

Cuts at:      0     832    1077 
   Size:        832     245
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15     446    1077 
   Size:         15     431     631

  Fragments arranged by size: 

                 631     431      15
 
BsiHKAI G_wGCw'C

Cuts at:      0      92    1044    1077 
   Size:         92     952      33

  Fragments arranged by size: 

                 952      92      33
 
BsmI GAATG_Cn'

Cuts at:      0     446    1001    1077 
   Size:        446     555      76

  Fragments arranged by size: 

                 555     446      76
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      91    1077 
   Size:         91     986
 
Bsp1286I G_dGCh'C

Cuts at:      0      92    1044    1077 
   Size:         92     952      33

  Fragments arranged by size: 

                 952      92      33
 
BspHI T'CATG_A

Cuts at:      0     363    1077 
   Size:        363     714
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     401    1077 
   Size:        401     676
 
BsrGI T'GTAC_A

Cuts at:      0     582    1077 
   Size:        582     495
 
BstBI TT'CG_AA

Cuts at:      0     168     606    1077 
   Size:        168     438     471

  Fragments arranged by size: 

                 471     438     168
 
BstZ17I GTA'TAC

Cuts at:      0     854    1077 
   Size:        854     223
 
Bsu36I CC'TnA_GG

Cuts at:      0     911    1077 
   Size:        911     166
 
DraI TTT'AAA

Cuts at:      0     920    1077 
   Size:        920     157
 
DraIII CAC_nnn'GTG

Cuts at:      0     330    1077 
   Size:        330     747
 
EarI CTCTTCn'nnn_

Cuts at:      0      10     186    1077 
   Size:         10     176     891

  Fragments arranged by size: 

                 891     176      10
 
EcoICRI GAG'CTC

Cuts at:      0    1042    1077 
   Size:       1042      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407    1077 
   Size:        407     670
 
EcoRI G'AATT_C

Cuts at:      0    1006    1077 
   Size:       1006      71
 
HaeII r_GCGC'y

Cuts at:      0     345    1077 
   Size:        345     732
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     468     500    1077 
   Size:        468      32     577

  Fragments arranged by size: 

                 577     468      32
 
KasI G'GCGC_C

Cuts at:      0     341    1077 
   Size:        341     736
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     414    1077 
   Size:        190     224     663

  Fragments arranged by size: 

                 663     224     190
 
MslI CAynn'nnrTG

Cuts at:      0     890     947    1077 
   Size:        890      57     130

  Fragments arranged by size: 

                 890     130      57
 
NarI GG'CG_CC

Cuts at:      0     342    1077 
   Size:        342     735
 
NsiI A_TGCA'T

Cuts at:      0     726    1077 
   Size:        726     351
 
PmlI CAC'GTG

Cuts at:      0     708    1077 
   Size:        708     369
 
PsiI TTA'TAA

Cuts at:      0      59    1077 
   Size:         59    1018
 
PstI C_TGCA'G

Cuts at:      0     537    1077 
   Size:        537     540
 
SacI G_AGCT'C

Cuts at:      0    1044    1077 
   Size:       1044      33
 
SapI GCTCTTCn'nnn_

Cuts at:      0     186    1077 
   Size:        186     891
 
SfcI C'TryA_G

Cuts at:      0     533    1077 
   Size:        533     544
 
SfoI GGC'GCC

Cuts at:      0     343    1077 
   Size:        343     734
 
SmlI C'TyrA_G

Cuts at:      0     733    1077 
   Size:        733     344
 
SwaI ATTT'AAAT

Cuts at:      0     920    1077 
   Size:        920     157
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     125    1077 
   Size:        125     952
 
TatI w'GTAC_w

Cuts at:      0     582    1077 
   Size:        582     495
 
Tth111I GACn'n_nGTC

Cuts at:      0     661    1077 
   Size:        661     416
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AloI     ApoI     AseI     BanI    BanII
     BbeI     BbsI    BfrBI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrGI
    BstBI  BstZ17I   Bsu36I     DraI   DraIII     EarI  EcoICRI  Eco57MI
    EcoRI    HaeII    Hin4I     KasI     MmeI     MslI     NarI     NsiI
     PmlI     PsiI     PstI     SacI     SapI     SfcI     SfoI     SmlI
     SwaI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsiEI    BsiWI    BspEI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI
     EagI     EciI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI