Restriction map for
pF1KB4447
(Linear) (Six-base) MAPSORT of: KIBB4447.seq Check: 5732 from: 1 to: 1281
KIBB4447.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 35 560 1281
Size: 35 525 721
Fragments arranged by size:
721 525 35
Acc65I G'GTAC_C
Cuts at: 0 766 1281
Size: 766 515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 236 463 761 809 1038 1281
Size: 106 130 227 298 48 229 243
Fragments arranged by size:
298 243 229 227 130 106 48
AflIII A'CryG_T
Cuts at: 0 693 1281
Size: 693 588
AhdI GACnn_n'nnGTC
Cuts at: 0 206 1281
Size: 206 1075
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 553 585 1281
Size: 553 32 696
Fragments arranged by size:
696 553 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1008 1126 1281
Size: 1008 118 155
Fragments arranged by size:
1008 155 118
ApaLI G'TGCA_C
Cuts at: 0 48 1281
Size: 48 1233
AvaI C'yCGr_G
Cuts at: 0 18 581 1281
Size: 18 563 700
Fragments arranged by size:
700 563 18
BanI G'GyrC_C
Cuts at: 0 80 455 766 783 1281
Size: 80 375 311 17 498
Fragments arranged by size:
498 375 311 80 17
BanII G_rGCy'C
Cuts at: 0 542 581 1015 1281
Size: 542 39 434 266
Fragments arranged by size:
542 434 266 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 749 1281
Size: 749 532
BbvCI CC'TCA_GC
Cuts at: 0 602 1281
Size: 602 679
BciVI GTATCCnnnnn_n'
Cuts at: 0 906 1281
Size: 906 375
BclI T'GATC_A
Cuts at: 0 165 1281
Size: 165 1116
Bme1580I G_kGCm'C
Cuts at: 0 52 1281
Size: 52 1229
BmrI ACTGGGnnnn_n'
Cuts at: 0 1077 1281
Size: 1077 204
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1112 1281
Size: 1112 169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 595 1281
Size: 204 391 686
Fragments arranged by size:
686 391 204
Bpu10I CC'TnA_GC
Cuts at: 0 602 1281
Size: 602 679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1209 1281
Size: 1051 158 72
Fragments arranged by size:
1051 158 72
BsaAI yAC'GTr
Cuts at: 0 696 1281
Size: 696 585
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 553 583 616 646 1281
Size: 553 30 33 30 635
Fragments arranged by size:
635 553 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 1281
Size: 130 1151
BseYI C'CCAG_C
Cuts at: 0 192 196 269 1008 1281
Size: 192 4 73 739 273
Fragments arranged by size:
739 273 192 73 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 904 1281
Size: 613 291 377
Fragments arranged by size:
613 377 291
BsiHKAI G_wGCw'C
Cuts at: 0 52 518 542 1281
Size: 52 466 24 739
Fragments arranged by size:
739 466 52 24
BsmI GAATG_Cn'
Cuts at: 0 1231 1281
Size: 1231 50
Bsp1286I G_dGCh'C
Cuts at: 0 52 518 542 581 1015 1281
Size: 52 466 24 39 434 266
Fragments arranged by size:
466 434 266 52 39 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 35 560 635 1134 1281
Size: 35 525 75 499 147
Fragments arranged by size:
525 499 147 75 35
BsrDI GCAATG_nn'
Cuts at: 0 168 1281
Size: 168 1113
BsrFI r'CCGG_y
Cuts at: 0 566 1281
Size: 566 715
BssSI C'ACGA_G
Cuts at: 0 466 1281
Size: 466 815
BstAPI GCAn_nnn'nTGC
Cuts at: 0 9 1281
Size: 9 1272
BstYI r'GATC_y
Cuts at: 0 654 935 1281
Size: 654 281 346
Fragments arranged by size:
654 346 281
Bsu36I CC'TnA_GG
Cuts at: 0 33 1281
Size: 33 1248
BtgI C'CryG_G
Cuts at: 0 275 864 1281
Size: 275 589 417
Fragments arranged by size:
589 417 275
BtsI GCAGTG_nn'
Cuts at: 0 82 371 438 827 1281
Size: 82 289 67 389 454
Fragments arranged by size:
454 389 289 82 67
DraIII CAC_nnn'GTG
Cuts at: 0 699 1281
Size: 699 582
EaeI y'GGCC_r
Cuts at: 0 332 608 956 1205 1281
Size: 332 276 348 249 76
Fragments arranged by size:
348 332 276 249 76
EarI CTCTTCn'nnn_
Cuts at: 0 547 1281
Size: 547 734
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 116 1281
Size: 116 1165
EcoICRI GAG'CTC
Cuts at: 0 540 1281
Size: 540 741
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 204 236 463 595 761 809 1038
Size: 106 98 32 227 132 166 48 229
Cuts at: 1038 1281
Size: 243
Fragments arranged by size:
243 229 227 166 132 106 98 48
32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 21 721 1281
Size: 21 700 560
Fragments arranged by size:
700 560 21
EcoO109I rG'GnC_Cy
Cuts at: 0 200 247 324 1123 1220 1281
Size: 200 47 77 799 97 61
Fragments arranged by size:
799 200 97 77 61 47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 408 525 1281
Size: 408 117 756
Fragments arranged by size:
756 408 117
HaeII r_GCGC'y
Cuts at: 0 567 617 1281
Size: 567 50 664
Fragments arranged by size:
664 567 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 192 224 736 768 1281
Size: 192 32 512 32 513
Fragments arranged by size:
513 512 192 32 32
HincII GTy'rAC
Cuts at: 0 528 1281
Size: 528 753
KpnI G_GTAC'C
Cuts at: 0 770 1281
Size: 770 511
MscI TGG'CCA
Cuts at: 0 334 610 1207 1281
Size: 334 276 597 74
Fragments arranged by size:
597 334 276 74
MspA1I CmG'CkG
Cuts at: 0 196 558 1005 1281
Size: 196 362 447 276
Fragments arranged by size:
447 362 276 196
NcoI C'CATG_G
Cuts at: 0 864 1281
Size: 864 417
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 1067 1281
Size: 420 647 214
Fragments arranged by size:
647 420 214
PfoI T'CCnGG_A
Cuts at: 0 572 1281
Size: 572 709
PmlI CAC'GTG
Cuts at: 0 696 1281
Size: 696 585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 553 585 1281
Size: 553 32 696
Fragments arranged by size:
696 553 32
PpuMI rG'GwC_Cy
Cuts at: 0 200 324 1123 1281
Size: 200 124 799 158
Fragments arranged by size:
799 200 158 124
PstI C_TGCA'G
Cuts at: 0 557 1281
Size: 557 724
PvuII CAG'CTG
Cuts at: 0 196 1281
Size: 196 1085
SacI G_AGCT'C
Cuts at: 0 542 1281
Size: 542 739
SanDI GG'GwC_CC
Cuts at: 0 200 1281
Size: 200 1081
SapI GCTCTTCn'nnn_
Cuts at: 0 547 1281
Size: 547 734
ScaI AGT'ACT
Cuts at: 0 778 1281
Size: 778 503
SfcI C'TryA_G
Cuts at: 0 174 376 553 882 931 1281
Size: 174 202 177 329 49 350
Fragments arranged by size:
350 329 202 177 174 49
SmaI CCC'GGG
Cuts at: 0 583 1281
Size: 583 698
SmlI C'TyrA_G
Cuts at: 0 1030 1224 1281
Size: 1030 194 57
Fragments arranged by size:
1030 194 57
StyI C'CwwG_G
Cuts at: 0 864 1281
Size: 864 417
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1174 1281
Size: 1174 107
TatI w'GTAC_w
Cuts at: 0 776 1281
Size: 776 505
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 683 715 817 849 1281
Size: 683 32 102 32 432
Fragments arranged by size:
683 432 102 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 523 1281
Size: 523 758
XmaI C'CCGG_G
Cuts at: 0 581 1281
Size: 581 700
XmnI GAAnn'nnTTC
Cuts at: 0 794 1281
Size: 794 487
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AloI AlwNI ApaLI
AvaI BanI BanII BbsI BbvCI BciVI BclI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI
BssSI BstAPI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII KpnI MscI MspA1I NcoI PflMI PfoI
PmlI PpiI PpuMI PstI PvuII SacI SanDI SapI
ScaI SfcI SmaI SmlI StyI TaqII TatI TstI
Tth111I XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AleI AlfI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BfrBI BglI BglII BlpI BmgBI BmtI
BsaI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XcmI XhoI
ZraI