Restriction map for
pF1KB4417
(Linear) (Six-base) MAPSORT of: KIBB4417.seq Check: 2242 from: 1 to: 1470
KIBB4417.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 943 1470
Size: 49 894 527
Fragments arranged by size:
894 527 49
AleI CACnn'nnGTG
Cuts at: 0 894 1470
Size: 894 576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 566 1351 1470
Size: 566 785 119
Fragments arranged by size:
785 566 119
AlwNI CAG_nnn'CTG
Cuts at: 0 27 459 537 582 1022 1097 1470
Size: 27 432 78 45 440 75 373
Fragments arranged by size:
440 432 373 78 75 45 27
ApaI G_GGCC'C
Cuts at: 0 209 512 1470
Size: 209 303 958
Fragments arranged by size:
958 303 209
AvaI C'yCGr_G
Cuts at: 0 722 1470
Size: 722 748
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1 34 1470
Size: 1 33 1436
Fragments arranged by size:
1436 33 1
BanI G'GyrC_C
Cuts at: 0 33 462 550 1327 1470
Size: 33 429 88 777 143
Fragments arranged by size:
777 429 143 88 33
BanII G_rGCy'C
Cuts at: 0 50 156 209 416 512 1470
Size: 50 106 53 207 96 958
Fragments arranged by size:
958 207 106 96 53 50
BbvCI CC'TCA_GC
Cuts at: 0 1434 1470
Size: 1434 36
BglI GCCn_nnn'nGGC
Cuts at: 0 507 770 874 1470
Size: 507 263 104 596
Fragments arranged by size:
596 507 263 104
BglII A'GATC_T
Cuts at: 0 737 839 1470
Size: 737 102 631
Fragments arranged by size:
737 631 102
Bme1580I G_kGCm'C
Cuts at: 0 209 512 555 1330 1470
Size: 209 303 43 775 140
Fragments arranged by size:
775 303 209 140 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 1410 1470
Size: 1410 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 899 1018 1399 1470
Size: 899 119 381 71
Fragments arranged by size:
899 381 119 71
Bpu10I CC'TnA_GC
Cuts at: 0 1434 1470
Size: 1434 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 719 1470
Size: 719 751
BsaHI Gr'CG_yC
Cuts at: 0 593 1070 1470
Size: 593 477 400
Fragments arranged by size:
593 477 400
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 352 382 1390 1420 1470
Size: 352 30 1008 30 50
Fragments arranged by size:
1008 352 50 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 448 1470
Size: 58 390 1022
Fragments arranged by size:
1022 390 58
BseYI C'CCAG_C
Cuts at: 0 110 219 327 422 502 512 687 1097
Size: 110 109 108 95 80 10 175 410
Cuts at: 1097 1470
Size: 373
Fragments arranged by size:
410 373 175 110 109 108 95 80
10
BsmI GAATG_Cn'
Cuts at: 0 263 1470
Size: 263 1207
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1312 1470
Size: 1312 158
Bsp1286I G_dGCh'C
Cuts at: 0 50 156 209 416 512 555 1330 1470
Size: 50 106 53 207 96 43 775 140
Fragments arranged by size:
775 207 140 106 96 53 50 43
BspHI T'CATG_A
Cuts at: 0 977 1470
Size: 977 493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 995 1470
Size: 536 459 475
Fragments arranged by size:
536 475 459
BsrBI CCG'CTC
Cuts at: 0 548 1470
Size: 548 922
BsrFI r'CCGG_y
Cuts at: 0 340 1470
Size: 340 1130
BsrGI T'GTAC_A
Cuts at: 0 701 1470
Size: 701 769
BstAPI GCAn_nnn'nTGC
Cuts at: 0 537 1457 1470
Size: 537 920 13
Fragments arranged by size:
920 537 13
BstXI CCAn_nnnn'nTGG
Cuts at: 0 791 1470
Size: 791 679
BstYI r'GATC_y
Cuts at: 0 737 839 1470
Size: 737 102 631
Fragments arranged by size:
737 631 102
Bsu36I CC'TnA_GG
Cuts at: 0 269 1393 1470
Size: 269 1124 77
Fragments arranged by size:
1124 269 77
BtgI C'CryG_G
Cuts at: 0 296 588 1202 1470
Size: 296 292 614 268
Fragments arranged by size:
614 296 292 268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 348 471 1470
Size: 348 123 999
Fragments arranged by size:
999 348 123
BtsI GCAGTG_nn'
Cuts at: 0 254 651 1470
Size: 254 397 819
Fragments arranged by size:
819 397 254
EaeI y'GGCC_r
Cuts at: 0 906 1470
Size: 906 564
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 538 1470
Size: 538 932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 899 943 1018 1399 1470
Size: 49 850 44 75 381 71
Fragments arranged by size:
850 381 75 71 49 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 763 1470
Size: 763 707
EcoO109I rG'GnC_Cy
Cuts at: 0 114 273 438 509 1470
Size: 114 159 165 71 961
Fragments arranged by size:
961 165 159 114 71
FspI TGC'GCA
Cuts at: 0 347 1470
Size: 347 1123
FspAI rTGC'GCAy
Cuts at: 0 347 1470
Size: 347 1123
HaeII r_GCGC'y
Cuts at: 0 219 1470
Size: 219 1251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 1390 1422 1470
Size: 352 32 1006 32 48
Fragments arranged by size:
1006 352 48 32 32
HincII GTy'rAC
Cuts at: 0 1242 1470
Size: 1242 228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1316 1470
Size: 1316 154
MslI CAynn'nnrTG
Cuts at: 0 490 894 1180 1470
Size: 490 404 286 290
Fragments arranged by size:
490 404 290 286
MspA1I CmG'CkG
Cuts at: 0 110 168 450 506 713 1094 1204 1377
Size: 110 58 282 56 207 381 110 173
Cuts at: 1377 1438 1470
Size: 61 32
Fragments arranged by size:
381 282 207 173 110 110 61 58
56 32
NcoI C'CATG_G
Cuts at: 0 588 1470
Size: 588 882
NdeI CA'TA_TG
Cuts at: 0 1230 1470
Size: 1230 240
NspI r_CATG'y
Cuts at: 0 576 1470
Size: 576 894
PflMI CCAn_nnn'nTGG
Cuts at: 0 611 1470
Size: 611 859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 846 878 1470
Size: 846 32 592
Fragments arranged by size:
846 592 32
PpuMI rG'GwC_Cy
Cuts at: 0 114 438 1470
Size: 114 324 1032
Fragments arranged by size:
1032 324 114
PshAI GACnn'nnGTC
Cuts at: 0 676 1470
Size: 676 794
PspOMI G'GGCC_C
Cuts at: 0 205 508 1470
Size: 205 303 962
Fragments arranged by size:
962 303 205
PstI C_TGCA'G
Cuts at: 0 42 455 533 545 712 1470
Size: 42 413 78 12 167 758
Fragments arranged by size:
758 413 167 78 42 12
PvuII CAG'CTG
Cuts at: 0 168 450 713 1094 1377 1438 1470
Size: 168 282 263 381 283 61 32
Fragments arranged by size:
381 283 282 263 168 61 32
SacII CC_GC'GG
Cuts at: 0 1205 1470
Size: 1205 265
SbfI CC_TGCA'GG
Cuts at: 0 533 1470
Size: 533 937
SfcI C'TryA_G
Cuts at: 0 38 451 529 541 708 1470
Size: 38 413 78 12 167 762
Fragments arranged by size:
762 413 167 78 38 12
SgrAI Cr'CCGG_yG
Cuts at: 0 340 1470
Size: 340 1130
SmlI C'TyrA_G
Cuts at: 0 722 1470
Size: 722 748
StuI AGG'CCT
Cuts at: 0 322 748 1470
Size: 322 426 722
Fragments arranged by size:
722 426 322
StyI C'CwwG_G
Cuts at: 0 588 1470
Size: 588 882
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 603 1470
Size: 415 188 867
Fragments arranged by size:
867 415 188
TatI w'GTAC_w
Cuts at: 0 701 1253 1470
Size: 701 552 217
Fragments arranged by size:
701 552 217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 612 1470
Size: 612 858
XhoI C'TCGA_G
Cuts at: 0 722 1470
Size: 722 748
XmnI GAAnn'nnTTC
Cuts at: 0 263 1470
Size: 263 1207
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI AvaI BaeI BanI
BanII BbvCI BglI BglII Bme1580I BmrI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BseYI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EciI Eco57MI EcoNI
EcoO109I FspI FspAI HaeII Hin4I HincII MmeI MslI
MspA1I NcoI NdeI NspI PflMI PpiI PpuMI PshAI
PspOMI PstI PvuII SacII SbfI SfcI SgrAI SmlI
StuI StyI TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbeI BbsI BcgI BciVI BclI BfrBI
BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI
BsgI BsiEI BsiHKAI BsiWI BspEI BsrDI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoRI EcoRV FalI FseI HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XmaI ZraI