Restriction map for
pF1KB4392
(Linear) (Six-base) MAPSORT of: KIBB4392.seq Check: 8602 from: 1 to: 1068
KIBB4392.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 716 1068
Size: 716 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1068
Size: 572 496
AfeI AGC'GCT
Cuts at: 0 159 1068
Size: 159 909
AgeI A'CCGG_T
Cuts at: 0 623 1068
Size: 623 445
AleI CACnn'nnGTG
Cuts at: 0 441 1068
Size: 441 627
AlwNI CAG_nnn'CTG
Cuts at: 0 37 1068
Size: 37 1031
AvaI C'yCGr_G
Cuts at: 0 82 119 413 452 771 827 911 941
Size: 82 37 294 39 319 56 84 30
Cuts at: 941 971 1060 1068
Size: 30 89 8
Fragments arranged by size:
319 294 89 84 82 56 39 37
30 30 8
BanI G'GyrC_C
Cuts at: 0 231 756 1068
Size: 231 525 312
Fragments arranged by size:
525 312 231
BanII G_rGCy'C
Cuts at: 0 616 1068
Size: 616 452
BbsI GAAGACnn'nnnn_
Cuts at: 0 276 1068
Size: 276 792
BbvCI CC'TCA_GC
Cuts at: 0 299 731 790 1068
Size: 299 432 59 278
Fragments arranged by size:
432 299 278 59
BciVI GTATCCnnnnn_n'
Cuts at: 0 314 930 1068
Size: 314 616 138
Fragments arranged by size:
616 314 138
BglII A'GATC_T
Cuts at: 0 175 1068
Size: 175 893
BlpI GC'TnA_GC
Cuts at: 0 542 1068
Size: 542 526
Bme1580I G_kGCm'C
Cuts at: 0 761 1068
Size: 761 307
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 176 986 1068
Size: 176 810 82
Fragments arranged by size:
810 176 82
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 750 1011 1068
Size: 628 122 261 57
Fragments arranged by size:
628 261 122 57
Bpu10I CC'TnA_GC
Cuts at: 0 299 731 790 1068
Size: 299 432 59 278
Fragments arranged by size:
432 299 278 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 850 1068
Size: 313 537 218
Fragments arranged by size:
537 313 218
BsaI GGTCTCn'nnnn_
Cuts at: 0 632 866 957 1068
Size: 632 234 91 111
Fragments arranged by size:
632 234 111 91
BsaWI w'CCGG_w
Cuts at: 0 317 623 1068
Size: 317 306 445
Fragments arranged by size:
445 317 306
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 404 1012 1027 1068
Size: 404 608 15 41
Fragments arranged by size:
608 404 41 15
BseYI C'CCAG_C
Cuts at: 0 30 43 394 800 819 905 927 952
Size: 30 13 351 406 19 86 22 25
Cuts at: 952 1068
Size: 116
Fragments arranged by size:
406 351 116 86 30 25 22 19
13
Bsp1286I G_dGCh'C
Cuts at: 0 616 761 1068
Size: 616 145 307
Fragments arranged by size:
616 307 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 198 356 716 1068
Size: 198 158 360 352
Fragments arranged by size:
360 352 198 158
BsrBI CCG'CTC
Cuts at: 0 457 1035 1068
Size: 457 578 33
Fragments arranged by size:
578 457 33
BsrFI r'CCGG_y
Cuts at: 0 623 1068
Size: 623 445
BstYI r'GATC_y
Cuts at: 0 175 286 1038 1068
Size: 175 111 752 30
Fragments arranged by size:
752 175 111 30
Bsu36I CC'TnA_GG
Cuts at: 0 569 1068
Size: 569 499
BtgI C'CryG_G
Cuts at: 0 492 597 1068
Size: 492 105 471
Fragments arranged by size:
492 471 105
BtsI GCAGTG_nn'
Cuts at: 0 254 698 1068
Size: 254 444 370
Fragments arranged by size:
444 370 254
EaeI y'GGCC_r
Cuts at: 0 977 1068
Size: 977 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 628 750 1011 1068
Size: 572 56 122 261 57
Fragments arranged by size:
572 261 122 57 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 1068
Size: 55 1013
EcoO109I rG'GnC_Cy
Cuts at: 0 135 345 727 823 1068
Size: 135 210 382 96 245
Fragments arranged by size:
382 245 210 135 96
HaeII r_GCGC'y
Cuts at: 0 161 1068
Size: 161 907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 1068
Size: 707 361
MscI TGG'CCA
Cuts at: 0 979 1068
Size: 979 89
MslI CAynn'nnrTG
Cuts at: 0 441 1068
Size: 441 627
MspA1I CmG'CkG
Cuts at: 0 472 1068
Size: 472 596
NcoI C'CATG_G
Cuts at: 0 597 1068
Size: 597 471
PpuMI rG'GwC_Cy
Cuts at: 0 345 727 823 1068
Size: 345 382 96 245
Fragments arranged by size:
382 345 245 96
PspXI vC'TCGA_Gb
Cuts at: 0 452 1060 1068
Size: 452 608 8
Fragments arranged by size:
608 452 8
PstI C_TGCA'G
Cuts at: 0 117 1068
Size: 117 951
PvuII CAG'CTG
Cuts at: 0 472 1068
Size: 472 596
SanDI GG'GwC_CC
Cuts at: 0 823 1068
Size: 823 245
ScaI AGT'ACT
Cuts at: 0 649 1068
Size: 649 419
SfcI C'TryA_G
Cuts at: 0 113 891 1068
Size: 113 778 177
Fragments arranged by size:
778 177 113
SmaI CCC'GGG
Cuts at: 0 415 773 829 1068
Size: 415 358 56 239
Fragments arranged by size:
415 358 239 56
SmlI C'TyrA_G
Cuts at: 0 292 452 865 1060 1068
Size: 292 160 413 195 8
Fragments arranged by size:
413 292 195 160 8
SrfI GCCC'GGGC
Cuts at: 0 773 1068
Size: 773 295
StyI C'CwwG_G
Cuts at: 0 597 1068
Size: 597 471
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 495 682 1068
Size: 495 187 386
Fragments arranged by size:
495 386 187
TatI w'GTAC_w
Cuts at: 0 647 1068
Size: 647 421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 679 711 1068
Size: 679 32 357
Fragments arranged by size:
679 357 32
XhoI C'TCGA_G
Cuts at: 0 452 1060 1068
Size: 452 608 8
Fragments arranged by size:
608 452 8
XmaI C'CCGG_G
Cuts at: 0 413 771 827 1068
Size: 413 358 56 241
Fragments arranged by size:
413 358 241 56
Enzymes that do cut:
AarI AcuI AfeI AgeI AleI AlwNI AvaI BanI
BanII BbsI BbvCI BciVI BglII BlpI Bme1580I BplI
BpmI Bpu10I BpuEI BsaI BsaWI BseRI BseYI Bsp1286I
BspMI BsrBI BsrFI BstYI Bsu36I BtgI BtsI EaeI
Eco57MI EcoNI EcoO109I HaeII MmeI MscI MslI MspA1I
NcoI PpuMI PspXI PstI PvuII SanDI ScaI SfcI
SmaI SmlI SrfI StyI TaqII TatI TstI XhoI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BclI BfrBI BglI BmgBI
BmrI BmtI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI Tth111I XbaI
XcmI XmnI ZraI