Restriction map for
pF1KB4391
(Linear) (Six-base) MAPSORT of: KIBB4391.seq Check: 9435 from: 1 to: 1560
KIBB4391.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 811 1560
Size: 505 306 749
Fragments arranged by size:
749 505 306
AfeI AGC'GCT
Cuts at: 0 1054 1560
Size: 1054 506
AflIII A'CryG_T
Cuts at: 0 499 1560
Size: 499 1061
AlwNI CAG_nnn'CTG
Cuts at: 0 29 1560
Size: 29 1531
ApaLI G'TGCA_C
Cuts at: 0 1381 1560
Size: 1381 179
ApoI r'AATT_y
Cuts at: 0 486 787 873 1285 1560
Size: 486 301 86 412 275
Fragments arranged by size:
486 412 301 275 86
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1265 1298 1560
Size: 1265 33 262
Fragments arranged by size:
1265 262 33
BanII G_rGCy'C
Cuts at: 0 22 1560
Size: 22 1538
BbsI GAAGACnn'nnnn_
Cuts at: 0 1093 1560
Size: 1093 467
BlpI GC'TnA_GC
Cuts at: 0 855 1560
Size: 855 705
Bme1580I G_kGCm'C
Cuts at: 0 1385 1560
Size: 1385 175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 967 1560
Size: 967 593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 1560
Size: 6 1554
Bpu10I CC'TnA_GC
Cuts at: 0 16 1560
Size: 16 1544
BsaAI yAC'GTr
Cuts at: 0 1281 1524 1560
Size: 1281 243 36
Fragments arranged by size:
1281 243 36
BsaHI Gr'CG_yC
Cuts at: 0 779 1560
Size: 779 781
BsaWI w'CCGG_w
Cuts at: 0 39 1560
Size: 39 1521
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 1560
Size: 4 30 1526
Fragments arranged by size:
1526 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1498 1560
Size: 1498 62
BseYI C'CCAG_C
Cuts at: 0 321 1560
Size: 321 1239
BsiHKAI G_wGCw'C
Cuts at: 0 22 1385 1560
Size: 22 1363 175
Fragments arranged by size:
1363 175 22
BsmI GAATG_Cn'
Cuts at: 0 516 947 1560
Size: 516 431 613
Fragments arranged by size:
613 516 431
Bsp1286I G_dGCh'C
Cuts at: 0 22 1385 1560
Size: 22 1363 175
Fragments arranged by size:
1363 175 22
BspEI T'CCGG_A
Cuts at: 0 39 1560
Size: 39 1521
BspHI T'CATG_A
Cuts at: 0 506 1560
Size: 506 1054
BsrBI CCG'CTC
Cuts at: 0 854 1560
Size: 854 706
BsrDI GCAATG_nn'
Cuts at: 0 32 1560
Size: 32 1528
BsrFI r'CCGG_y
Cuts at: 0 95 1560
Size: 95 1465
BstAPI GCAn_nnn'nTGC
Cuts at: 0 92 1560
Size: 92 1468
BtgI C'CryG_G
Cuts at: 0 575 1386 1560
Size: 575 811 174
Fragments arranged by size:
811 575 174
BtsI GCAGTG_nn'
Cuts at: 0 458 1560
Size: 458 1102
DraIII CAC_nnn'GTG
Cuts at: 0 679 1560
Size: 679 881
EaeI y'GGCC_r
Cuts at: 0 162 1560
Size: 162 1398
EarI CTCTTCn'nnn_
Cuts at: 0 653 1560
Size: 653 907
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 624 1560
Size: 624 936
EcoICRI GAG'CTC
Cuts at: 0 20 1560
Size: 20 1540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 505 811 1560
Size: 6 499 306 749
Fragments arranged by size:
749 499 306 6
EcoRI G'AATT_C
Cuts at: 0 787 873 1560
Size: 787 86 687
Fragments arranged by size:
787 687 86
HaeII r_GCGC'y
Cuts at: 0 1056 1560
Size: 1056 504
HincII GTy'rAC
Cuts at: 0 966 1560
Size: 966 594
HpaI GTT'AAC
Cuts at: 0 966 1560
Size: 966 594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 1560
Size: 125 1435
MscI TGG'CCA
Cuts at: 0 164 1560
Size: 164 1396
MslI CAynn'nnrTG
Cuts at: 0 1454 1560
Size: 1454 106
MspA1I CmG'CkG
Cuts at: 0 382 1372 1442 1560
Size: 382 990 70 118
Fragments arranged by size:
990 382 118 70
NcoI C'CATG_G
Cuts at: 0 1386 1560
Size: 1386 174
NdeI CA'TA_TG
Cuts at: 0 976 1560
Size: 976 584
NspI r_CATG'y
Cuts at: 0 503 1560
Size: 503 1057
PciI A'CATG_T
Cuts at: 0 499 1560
Size: 499 1061
PstI C_TGCA'G
Cuts at: 0 88 869 1560
Size: 88 781 691
Fragments arranged by size:
781 691 88
PvuII CAG'CTG
Cuts at: 0 382 1560
Size: 382 1178
SacI G_AGCT'C
Cuts at: 0 22 1560
Size: 22 1538
ScaI AGT'ACT
Cuts at: 0 1464 1560
Size: 1464 96
SfcI C'TryA_G
Cuts at: 0 84 270 865 1560
Size: 84 186 595 695
Fragments arranged by size:
695 595 186 84
SnaBI TAC'GTA
Cuts at: 0 1281 1560
Size: 1281 279
StuI AGG'CCT
Cuts at: 0 479 1301 1507 1560
Size: 479 822 206 53
Fragments arranged by size:
822 479 206 53
StyI C'CwwG_G
Cuts at: 0 112 630 1211 1386 1560
Size: 112 518 581 175 174
Fragments arranged by size:
581 518 175 174 112
TatI w'GTAC_w
Cuts at: 0 274 1462 1560
Size: 274 1188 98
Fragments arranged by size:
1188 274 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4 36 140 172 1375 1407 1560
Size: 4 32 104 32 1203 32 153
Fragments arranged by size:
1203 153 104 32 32 32 4
XbaI T'CTAG_A
Cuts at: 0 991 1560
Size: 991 569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 1540 1560
Size: 324 1216 20
Fragments arranged by size:
1216 324 20
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApaLI ApoI BaeI BanII
BbsI BlpI Bme1580I BplI BpmI Bpu10I BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrFI BstAPI BtgI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoRI HaeII HincII
HpaI MmeI MscI MslI MspA1I NcoI NdeI NspI
PciI PstI PvuII SacI ScaI SfcI SnaBI StuI
StyI TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmrI BmtI BpuEI BsaI
BsaBI BsgI BsiEI BsiWI BsmBI BspMI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EcoNI EcoO109I EcoRV
FalI FseI FspI FspAI Hin4I HindIII KasI KpnI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XhoI XmaI XmnI ZraI