Restriction map for
pF1KB4367
(Linear) (Six-base) MAPSORT of: KIBB4367.seq Check: 4623 from: 1 to: 3372
KIBB4367.orf
With 182 enzymes: SgfI *
July 23, 2007 10:03 ..
AclI AA'CG_TT
Cuts at: 0 56 3372
Size: 56 3316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 449 1067 3287 3371 3372
Size: 308 141 618 2220 84 1
Fragments arranged by size:
2220 618 308 141 84 1
AfeI AGC'GCT
Cuts at: 0 2483 2902 3372
Size: 2483 419 470
Fragments arranged by size:
2483 470 419
AflII C'TTAA_G
Cuts at: 0 774 2475 3372
Size: 774 1701 897
Fragments arranged by size:
1701 897 774
AflIII A'CryG_T
Cuts at: 0 687 3372
Size: 687 2685
AleI CACnn'nnGTG
Cuts at: 0 2440 3032 3372
Size: 2440 592 340
Fragments arranged by size:
2440 592 340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3050 3372
Size: 3050 322
AlwNI CAG_nnn'CTG
Cuts at: 0 1696 1729 2422 3007 3372
Size: 1696 33 693 585 365
Fragments arranged by size:
1696 693 585 365 33
ApaLI G'TGCA_C
Cuts at: 0 2174 3372
Size: 2174 1198
ApoI r'AATT_y
Cuts at: 0 87 715 779 1060 1470 3372
Size: 87 628 64 281 410 1902
Fragments arranged by size:
1902 628 410 281 87 64
AseI AT'TA_AT
Cuts at: 0 1622 3372
Size: 1622 1750
BanI G'GyrC_C
Cuts at: 0 423 3034 3372
Size: 423 2611 338
Fragments arranged by size:
2611 423 338
BanII G_rGCy'C
Cuts at: 0 1832 3372
Size: 1832 1540
BbsI GAAGACnn'nnnn_
Cuts at: 0 113 1375 1585 1913 2698 3107 3372
Size: 113 1262 210 328 785 409 265
Fragments arranged by size:
1262 785 409 328 265 210 113
BbvCI CC'TCA_GC
Cuts at: 0 2168 3372
Size: 2168 1204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 325 359 3372
Size: 325 34 3013
Fragments arranged by size:
3013 325 34
BclI T'GATC_A
Cuts at: 0 1395 1977 3372
Size: 1395 582 1395
Fragments arranged by size:
1395 1395 582
BfrBI ATG'CAT
Cuts at: 0 493 3372
Size: 493 2879
BglII A'GATC_T
Cuts at: 0 1530 3372
Size: 1530 1842
Bme1580I G_kGCm'C
Cuts at: 0 451 2178 3372
Size: 451 1727 1194
Fragments arranged by size:
1727 1194 451
BmrI ACTGGGnnnn_n'
Cuts at: 0 2053 2421 3372
Size: 2053 368 951
Fragments arranged by size:
2053 951 368
BmtI G_CTAG'C
Cuts at: 0 2488 3372
Size: 2488 884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1751 2885 3372
Size: 1751 1134 487
Fragments arranged by size:
1751 1134 487
Bpu10I CC'TnA_GC
Cuts at: 0 293 2168 3312 3372
Size: 293 1875 1144 60
Fragments arranged by size:
1875 1144 293 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1456 3372
Size: 901 555 1916
Fragments arranged by size:
1916 901 555
BsaAI yAC'GTr
Cuts at: 0 3030 3372
Size: 3030 342
BsaHI Gr'CG_yC
Cuts at: 0 3043 3372
Size: 3043 329
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 201 231 1140 1170 1656 1686 3372
Size: 201 30 909 30 486 30 1686
Fragments arranged by size:
1686 909 486 201 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 225 1148 1664 3136 3139 3208 3211 3214
Size: 225 923 516 1472 3 69 3 3
Cuts at: 3214 3217 3372
Size: 3 155
Fragments arranged by size:
1472 923 516 225 155 69 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 245 3372
Size: 245 3127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 633 1841 1936 3372
Size: 633 1208 95 1436
Fragments arranged by size:
1436 1208 633 95
BsiHKAI G_wGCw'C
Cuts at: 0 773 1832 2178 3029 3372
Size: 773 1059 346 851 343
Fragments arranged by size:
1059 851 773 346 343
BsmI GAATG_Cn'
Cuts at: 0 237 337 811 1812 3372
Size: 237 100 474 1001 1560
Fragments arranged by size:
1560 1001 474 237 100
BsmBI CGTCTCn'nnnn_
Cuts at: 0 947 3050 3372
Size: 947 2103 322
Fragments arranged by size:
2103 947 322
Bsp1286I G_dGCh'C
Cuts at: 0 451 773 1832 2178 3029 3372
Size: 451 322 1059 346 851 343
Fragments arranged by size:
1059 851 451 346 343 322
BspHI T'CATG_A
Cuts at: 0 468 576 2793 3372
Size: 468 108 2217 579
Fragments arranged by size:
2217 579 468 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 188 3372
Size: 188 3184
BsrDI GCAATG_nn'
Cuts at: 0 2447 3372
Size: 2447 925
BsrGI T'GTAC_A
Cuts at: 0 313 2159 3372
Size: 313 1846 1213
Fragments arranged by size:
1846 1213 313
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1798 3372
Size: 1798 1574
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2442 2590 3372
Size: 2442 148 782
Fragments arranged by size:
2442 782 148
BstYI r'GATC_y
Cuts at: 0 1530 3372
Size: 1530 1842
Bsu36I CC'TnA_GG
Cuts at: 0 419 1160 3372
Size: 419 741 2212
Fragments arranged by size:
2212 741 419
BtgI C'CryG_G
Cuts at: 0 43 3372
Size: 43 3329
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2926 3372
Size: 2926 446
BtsI GCAGTG_nn'
Cuts at: 0 222 401 2420 3372
Size: 222 179 2019 952
Fragments arranged by size:
2019 952 222 179
DraI TTT'AAA
Cuts at: 0 1475 1766 2823 3372
Size: 1475 291 1057 549
Fragments arranged by size:
1475 1057 549 291
DraIII CAC_nnn'GTG
Cuts at: 0 731 2386 3372
Size: 731 1655 986
Fragments arranged by size:
1655 986 731
DrdI GACnn_nn'nnGTC
Cuts at: 0 406 3372
Size: 406 2966
EaeI y'GGCC_r
Cuts at: 0 1356 2444 3372
Size: 1356 1088 928
Fragments arranged by size:
1356 1088 928
EarI CTCTTCn'nnn_
Cuts at: 0 374 425 669 1820 2177 2990 3086 3113
Size: 374 51 244 1151 357 813 96 27
Cuts at: 3113 3185 3218 3227 3372
Size: 72 33 9 145
Fragments arranged by size:
1151 813 374 357 244 145 96 72
51 33 27 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 3372
Size: 19 3353
EcoICRI GAG'CTC
Cuts at: 0 1830 3372
Size: 1830 1542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 449 1067 1751 2885 3287 3371 3372
Size: 308 141 618 684 1134 402 84 1
Fragments arranged by size:
1134 684 618 402 308 141 84 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1943 3372
Size: 1943 1429
EcoO109I rG'GnC_Cy
Cuts at: 0 283 3004 3372
Size: 283 2721 368
Fragments arranged by size:
2721 368 283
EcoRI G'AATT_C
Cuts at: 0 779 1060 3372
Size: 779 281 2312
Fragments arranged by size:
2312 779 281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 843 1036 1321 1791 3372
Size: 843 193 285 470 1581
Fragments arranged by size:
1581 843 470 285 193
FspI TGC'GCA
Cuts at: 0 2316 3372
Size: 2316 1056
HaeII r_GCGC'y
Cuts at: 0 2485 2904 3372
Size: 2485 419 468
Fragments arranged by size:
2485 468 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2605 2637 3331 3363 3372
Size: 2605 32 694 32 9
Fragments arranged by size:
2605 694 32 32 9
HincII GTy'rAC
Cuts at: 0 1029 2249 3372
Size: 1029 1220 1123
Fragments arranged by size:
1220 1123 1029
HindIII A'AGCT_T
Cuts at: 0 740 1560 3372
Size: 740 820 1812
Fragments arranged by size:
1812 820 740
HpaI GTT'AAC
Cuts at: 0 1029 3372
Size: 1029 2343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1265 3372
Size: 1265 2107
MscI TGG'CCA
Cuts at: 0 1358 2446 3372
Size: 1358 1088 926
Fragments arranged by size:
1358 1088 926
MslI CAynn'nnrTG
Cuts at: 0 804 927 2440 2768 2798 3032 3372
Size: 804 123 1513 328 30 234 340
Fragments arranged by size:
1513 804 340 328 234 123 30
MspA1I CmG'CkG
Cuts at: 0 45 151 2167 3372
Size: 45 106 2016 1205
Fragments arranged by size:
2016 1205 106 45
NdeI CA'TA_TG
Cuts at: 0 801 3372
Size: 801 2571
NheI G'CTAG_C
Cuts at: 0 2484 3372
Size: 2484 888
NsiI A_TGCA'T
Cuts at: 0 495 3372
Size: 495 2877
NspI r_CATG'y
Cuts at: 0 691 2853 3372
Size: 691 2162 519
Fragments arranged by size:
2162 691 519
PciI A'CATG_T
Cuts at: 0 687 3372
Size: 687 2685
PflMI CCAn_nnn'nTGG
Cuts at: 0 1195 3372
Size: 1195 2177
PfoI T'CCnGG_A
Cuts at: 0 1611 2288 3372
Size: 1611 677 1084
Fragments arranged by size:
1611 1084 677
PmlI CAC'GTG
Cuts at: 0 3030 3372
Size: 3030 342
PsiI TTA'TAA
Cuts at: 0 68 3372
Size: 68 3304
PstI C_TGCA'G
Cuts at: 0 1689 2556 3372
Size: 1689 867 816
Fragments arranged by size:
1689 867 816
PvuII CAG'CTG
Cuts at: 0 151 2167 3372
Size: 151 2016 1205
Fragments arranged by size:
2016 1205 151
SacI G_AGCT'C
Cuts at: 0 1832 3372
Size: 1832 1540
SacII CC_GC'GG
Cuts at: 0 46 3372
Size: 46 3326
SapI GCTCTTCn'nnn_
Cuts at: 0 1820 2177 3372
Size: 1820 357 1195
Fragments arranged by size:
1820 1195 357
SfcI C'TryA_G
Cuts at: 0 846 1428 1685 2414 2552 3372
Size: 846 582 257 729 138 820
Fragments arranged by size:
846 820 729 582 257 138
SmlI C'TyrA_G
Cuts at: 0 774 916 1435 2475 3372
Size: 774 142 519 1040 897
Fragments arranged by size:
1040 897 774 519 142
SpeI A'CTAG_T
Cuts at: 0 2257 3372
Size: 2257 1115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 692 1356 3022 3372
Size: 692 664 1666 350
Fragments arranged by size:
1666 692 664 350
TatI w'GTAC_w
Cuts at: 0 313 1242 2101 2159 3372
Size: 313 929 859 58 1213
Fragments arranged by size:
1213 929 859 313 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 201 233 3372
Size: 201 32 3139
Fragments arranged by size:
3139 201 32
Tth111I GACn'n_nGTC
Cuts at: 0 696 3372
Size: 696 2676
XmnI GAAnn'nnTTC
Cuts at: 0 383 3372
Size: 383 2989
Enzymes that do cut:
AclI AcuI AfeI AflII AflIII AleI AlfI AlwNI
ApaLI ApoI AseI BanI BanII BbsI BbvCI BcgI
BclI BfrBI BglII Bme1580I BmrI BmtI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
MmeI MscI MslI MspA1I NdeI NheI NsiI NspI
PciI PflMI PfoI PmlI PsiI PstI PvuII SacI
SacII SapI SfcI SmlI SpeI TaqII TatI TstI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AgeI AhdI AloI ApaI
AscI AsiSI AvaI AvrII BaeI BamHI BbeI BciVI
BglI BlpI BmgBI BplI BsaI BsaBI BsaWI BsiEI
BsiWI BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII
BstZ17I ClaI CspCI EagI EcoRV FseI FspAI KasI
KpnI MfeI MluI NaeI NarI NcoI NgoMIV NotI
NruI PacI PasI PmeI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SspI StuI StyI SwaI XbaI XcmI XhoI
XmaI ZraI