(Linear) (Six-base) MAPSORT of: KIBB4347.seq  Check: 9447  from: 1  to: 636



KIBB4347.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     636 
   Size:        154     482
 
ApoI r'AATT_y

Cuts at:      0     497     509     636 
   Size:        497      12     127

  Fragments arranged by size: 

                 497     127      12
 
AseI AT'TA_AT

Cuts at:      0     482     533     636 
   Size:        482      51     103

  Fragments arranged by size: 

                 482     103      51
 
BbvCI CC'TCA_GC

Cuts at:      0     566     636 
   Size:        566      70
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     174     636 
   Size:        174     462
 
Bpu10I CC'TnA_GC

Cuts at:      0     566     636 
   Size:        566      70
 
BseYI C'CCAG_C

Cuts at:      0     619     636 
   Size:        619      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     636 
   Size:        254     382
 
BsiHKAI G_wGCw'C

Cuts at:      0     135     636 
   Size:        135     501
 
BsiWI C'GTAC_G

Cuts at:      0       5     636 
   Size:          5     631
 
BsmI GAATG_Cn'

Cuts at:      0     318     636 
   Size:        318     318
 
Bsp1286I G_dGCh'C

Cuts at:      0     135     636 
   Size:        135     501
 
BssSI C'ACGA_G

Cuts at:      0     413     636 
   Size:        413     223
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     592     636 
   Size:        592      44
 
EarI CTCTTCn'nnn_

Cuts at:      0      22     372     464     636 
   Size:         22     350      92     172

  Fragments arranged by size: 

                 350     172      92      22
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     636 
   Size:        154     482
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     355     387     636 
   Size:        355      32     249

  Fragments arranged by size: 

                 355     249      32
 
HindIII A'AGCT_T

Cuts at:      0     404     636 
   Size:        404     232
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     636 
   Size:        353     283
 
MslI CAynn'nnrTG

Cuts at:      0     433     636 
   Size:        433     203
 
NspI r_CATG'y

Cuts at:      0     574     595     636 
   Size:        574      21      41

  Fragments arranged by size: 

                 574      41      21
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     410     442     636 
   Size:        410      32     194

  Fragments arranged by size: 

                 410     194      32
 
SexAI A'CCwGG_T

Cuts at:      0     295     636 
   Size:        295     341
 
SfcI C'TryA_G

Cuts at:      0      70     636 
   Size:         70     566
 
SpeI A'CTAG_T

Cuts at:      0     249     636 
   Size:        249     387
 
SphI G_CATG'C

Cuts at:      0     574     636 
   Size:        574      62
 
TatI w'GTAC_w

Cuts at:      0      23     636 
   Size:         23     613
 
XbaI T'CTAG_A

Cuts at:      0     373     636 
   Size:        373     263
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     330     636 
   Size:        330     306
 
XmnI GAAnn'nnTTC

Cuts at:      0     200     509     636 
   Size:        200     309     127

  Fragments arranged by size: 

                 309     200     127
 

 Enzymes that do cut:

     AcuI     ApoI     AseI    BbvCI    BciVI   Bpu10I    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BssSI   BstAPI     EarI  Eco57MI
    Hin4I  HindIII     MmeI     MslI     NspI     PpiI    SexAI     SfcI
     SpeI     SphI     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XhoI     XmaI     ZraI