Restriction map for
pF1KB4327
(Linear) (Six-base) MAPSORT of: KIBB4327.seq Check: 5315 from: 1 to: 1614
KIBB4327.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 285 1614
Size: 285 1329
AccI GT'mk_AC
Cuts at: 0 515 1614
Size: 515 1099
AfeI AGC'GCT
Cuts at: 0 120 1614
Size: 120 1494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1489 1516 1614
Size: 1489 27 98
Fragments arranged by size:
1489 98 27
AlwNI CAG_nnn'CTG
Cuts at: 0 807 840 1614
Size: 807 33 774
Fragments arranged by size:
807 774 33
ApaI G_GGCC'C
Cuts at: 0 418 1614
Size: 418 1196
AvaI C'yCGr_G
Cuts at: 0 1504 1614
Size: 1504 110
BamHI G'GATC_C
Cuts at: 0 770 1614
Size: 770 844
BanI G'GyrC_C
Cuts at: 0 27 1036 1614
Size: 27 1009 578
Fragments arranged by size:
1009 578 27
BanII G_rGCy'C
Cuts at: 0 418 1000 1194 1259 1614
Size: 418 582 194 65 355
Fragments arranged by size:
582 418 355 194 65
BbeI G_GCGC'C
Cuts at: 0 31 1614
Size: 31 1583
BbsI GAAGACnn'nnnn_
Cuts at: 0 1050 1614
Size: 1050 564
BbvCI CC'TCA_GC
Cuts at: 0 766 1285 1304 1614
Size: 766 519 19 310
Fragments arranged by size:
766 519 310 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 165 199 1614
Size: 165 34 1415
Fragments arranged by size:
1415 165 34
BclI T'GATC_A
Cuts at: 0 656 1346 1614
Size: 656 690 268
Fragments arranged by size:
690 656 268
BglI GCCn_nnn'nGGC
Cuts at: 0 798 826 1094 1614
Size: 798 28 268 520
Fragments arranged by size:
798 520 268 28
BglII A'GATC_T
Cuts at: 0 1215 1614
Size: 1215 399
BlpI GC'TnA_GC
Cuts at: 0 779 1614
Size: 779 835
Bme1580I G_kGCm'C
Cuts at: 0 418 599 1614
Size: 418 181 1015
Fragments arranged by size:
1015 418 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 550 730 862 1614
Size: 325 225 180 132 752
Fragments arranged by size:
752 325 225 180 132
Bpu10I CC'TnA_GC
Cuts at: 0 766 1285 1304 1614
Size: 766 519 19 310
Fragments arranged by size:
766 519 310 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1614
Size: 196 1418
BsaI GGTCTCn'nnnn_
Cuts at: 0 1477 1614
Size: 1477 137
BsaAI yAC'GTr
Cuts at: 0 1428 1614
Size: 1428 186
BsaHI Gr'CG_yC
Cuts at: 0 28 104 458 1614
Size: 28 76 354 1156
Fragments arranged by size:
1156 354 76 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 742 772 1614
Size: 742 30 842
Fragments arranged by size:
842 742 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 438 763 829 883 1001 1004 1254 1441
Size: 438 325 66 54 118 3 250 187
Cuts at: 1441 1614
Size: 173
Fragments arranged by size:
438 325 250 187 173 118 66 54
3
BseYI C'CCAG_C
Cuts at: 0 77 807 1146 1388 1614
Size: 77 730 339 242 226
Fragments arranged by size:
730 339 242 226 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 1614
Size: 195 1419
BsiHKAI G_wGCw'C
Cuts at: 0 245 1021 1514 1614
Size: 245 776 493 100
Fragments arranged by size:
776 493 245 100
BsmI GAATG_Cn'
Cuts at: 0 1457 1614
Size: 1457 157
Bsp1286I G_dGCh'C
Cuts at: 0 245 418 599 1000 1021 1194 1259 1514
Size: 245 173 181 401 21 173 65 255
Cuts at: 1514 1614
Size: 100
Fragments arranged by size:
401 255 245 181 173 173 100 65
21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 285 859 1614
Size: 285 574 755
Fragments arranged by size:
755 574 285
BsrBI CCG'CTC
Cuts at: 0 664 1379 1614
Size: 664 715 235
Fragments arranged by size:
715 664 235
BsrDI GCAATG_nn'
Cuts at: 0 39 204 947 1127 1614
Size: 39 165 743 180 487
Fragments arranged by size:
743 487 180 165 39
BsrFI r'CCGG_y
Cuts at: 0 496 788 1123 1535 1614
Size: 496 292 335 412 79
Fragments arranged by size:
496 412 335 292 79
BssSI C'ACGA_G
Cuts at: 0 171 1614
Size: 171 1443
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1431 1614
Size: 1431 183
BstEII G'GTnAC_C
Cuts at: 0 355 1068 1614
Size: 355 713 546
Fragments arranged by size:
713 546 355
BstYI r'GATC_y
Cuts at: 0 270 311 770 1215 1614
Size: 270 41 459 445 399
Fragments arranged by size:
459 445 399 270 41
BtgI C'CryG_G
Cuts at: 0 795 1073 1332 1407 1614
Size: 795 278 259 75 207
Fragments arranged by size:
795 278 259 207 75
BtsI GCAGTG_nn'
Cuts at: 0 966 1614
Size: 966 648
DraIII CAC_nnn'GTG
Cuts at: 0 1187 1431 1614
Size: 1187 244 183
Fragments arranged by size:
1187 244 183
DrdI GACnn_nn'nnGTC
Cuts at: 0 511 1614
Size: 511 1103
EaeI y'GGCC_r
Cuts at: 0 261 375 1028 1086 1614
Size: 261 114 653 58 528
Fragments arranged by size:
653 528 261 114 58
EarI CTCTTCn'nnn_
Cuts at: 0 322 682 1264 1614
Size: 322 360 582 350
Fragments arranged by size:
582 360 350 322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 550 730 862 1614
Size: 325 225 180 132 752
Fragments arranged by size:
752 325 225 180 132
EcoNI CCTnn'n_nnAGG
Cuts at: 0 983 1504 1614
Size: 983 521 110
Fragments arranged by size:
983 521 110
EcoO109I rG'GnC_Cy
Cuts at: 0 53 415 828 837 1614
Size: 53 362 413 9 777
Fragments arranged by size:
777 413 362 53 9
HaeII r_GCGC'y
Cuts at: 0 31 122 140 1366 1395 1614
Size: 31 91 18 1226 29 219
Fragments arranged by size:
1226 219 91 31 29 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 648 680 742 774 1338 1370 1614
Size: 648 32 62 32 564 32 244
Fragments arranged by size:
648 564 244 62 32 32 32
HincII GTy'rAC
Cuts at: 0 504 1614
Size: 504 1110
KasI G'GCGC_C
Cuts at: 0 27 1614
Size: 27 1587
MscI TGG'CCA
Cuts at: 0 1030 1614
Size: 1030 584
MspA1I CmG'CkG
Cuts at: 0 36 101 179 1034 1571 1587 1614
Size: 36 65 78 855 537 16 27
Fragments arranged by size:
855 537 78 65 36 27 16
NaeI GCC'GGC
Cuts at: 0 1537 1614
Size: 1537 77
NarI GG'CG_CC
Cuts at: 0 28 1614
Size: 28 1586
NcoI C'CATG_G
Cuts at: 0 795 1407 1614
Size: 795 612 207
Fragments arranged by size:
795 612 207
NgoMIV G'CCGG_C
Cuts at: 0 1535 1614
Size: 1535 79
PflMI CCAn_nnn'nTGG
Cuts at: 0 981 1187 1614
Size: 981 206 427
Fragments arranged by size:
981 427 206
PfoI T'CCnGG_A
Cuts at: 0 302 707 1514 1614
Size: 302 405 807 100
Fragments arranged by size:
807 405 302 100
PmlI CAC'GTG
Cuts at: 0 1428 1614
Size: 1428 186
PpuMI rG'GwC_Cy
Cuts at: 0 837 1614
Size: 837 777
PspOMI G'GGCC_C
Cuts at: 0 414 1614
Size: 414 1200
PstI C_TGCA'G
Cuts at: 0 444 836 1424 1614
Size: 444 392 588 190
Fragments arranged by size:
588 444 392 190
SapI GCTCTTCn'nnn_
Cuts at: 0 1264 1614
Size: 1264 350
SbfI CC_TGCA'GG
Cuts at: 0 836 1614
Size: 836 778
SfcI C'TryA_G
Cuts at: 0 440 646 832 1420 1614
Size: 440 206 186 588 194
Fragments arranged by size:
588 440 206 194 186
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 826 1614
Size: 826 788
SfoI GGC'GCC
Cuts at: 0 29 1614
Size: 29 1585
SmlI C'TyrA_G
Cuts at: 0 211 1614
Size: 211 1403
StyI C'CwwG_G
Cuts at: 0 401 795 1251 1407 1614
Size: 401 394 456 156 207
Fragments arranged by size:
456 401 394 207 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 499 1094 1614
Size: 499 595 520
Fragments arranged by size:
595 520 499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 262 294 1614
Size: 262 32 1320
Fragments arranged by size:
1320 262 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 21 459 1405 1614
Size: 21 438 946 209
Fragments arranged by size:
946 438 209 21
Enzymes that do cut:
AarI AccI AfeI AlfI AlwNI ApaI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstYI
BtgI BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI MscI MspA1I NaeI
NarI NcoI NgoMIV PflMI PfoI PmlI PpuMI PspOMI
PstI SapI SbfI SfcI SfiI SfoI SmlI StyI
TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BciVI BfrBI BmgBI BmrI BmtI BplI BsaBI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrGI BssHII
BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MmeI MslI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI ScaI SexAI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XhoI XmaI XmnI
ZraI