Restriction map for
pF1KB4308
(Linear) (Six-base) MAPSORT of: KIBB4308.seq Check: 5260 from: 1 to: 549
KIBB4308.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 549
Size: 487 62
AleI CACnn'nnGTG
Cuts at: 0 287 549
Size: 287 262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 425 549
Size: 425 124
AlwNI CAG_nnn'CTG
Cuts at: 0 75 246 317 549
Size: 75 171 71 232
Fragments arranged by size:
232 171 75 71
ApaI G_GGCC'C
Cuts at: 0 32 549
Size: 32 517
BanI G'GyrC_C
Cuts at: 0 289 549
Size: 289 260
BanII G_rGCy'C
Cuts at: 0 32 549
Size: 32 517
BglI GCCn_nnn'nGGC
Cuts at: 0 469 549
Size: 469 80
Bme1580I G_kGCm'C
Cuts at: 0 32 227 326 549
Size: 32 195 99 223
Fragments arranged by size:
223 195 99 32
BmtI G_CTAG'C
Cuts at: 0 499 549
Size: 499 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 549
Size: 97 452
BsaI GGTCTCn'nnnn_
Cuts at: 0 119 549
Size: 119 430
BsaHI Gr'CG_yC
Cuts at: 0 7 549
Size: 7 542
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 549
Size: 421 30 98
Fragments arranged by size:
421 98 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 433 549
Size: 94 339 116
Fragments arranged by size:
339 116 94
BseYI C'CCAG_C
Cuts at: 0 46 459 549
Size: 46 413 90
Fragments arranged by size:
413 90 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 286 549
Size: 187 99 263
Fragments arranged by size:
263 187 99
BsiEI CG_ry'CG
Cuts at: 0 188 549
Size: 188 361
BsiHKAI G_wGCw'C
Cuts at: 0 59 549
Size: 59 490
Bsp1286I G_dGCh'C
Cuts at: 0 32 59 227 326 549
Size: 32 27 168 99 223
Fragments arranged by size:
223 168 99 32 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 515 536 545 549
Size: 515 21 9 4
Fragments arranged by size:
515 21 9 4
BsrDI GCAATG_nn'
Cuts at: 0 225 549
Size: 225 324
BsrFI r'CCGG_y
Cuts at: 0 187 549
Size: 187 362
BstXI CCAn_nnnn'nTGG
Cuts at: 0 375 549
Size: 375 174
BtgI C'CryG_G
Cuts at: 0 282 549
Size: 282 267
BtsI GCAGTG_nn'
Cuts at: 0 194 293 549
Size: 194 99 256
Fragments arranged by size:
256 194 99
EaeI y'GGCC_r
Cuts at: 0 185 549
Size: 185 364
EagI C'GGCC_G
Cuts at: 0 185 549
Size: 185 364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 487 549
Size: 97 390 62
Fragments arranged by size:
390 97 62
EcoO109I rG'GnC_Cy
Cuts at: 0 29 165 534 549
Size: 29 136 369 15
Fragments arranged by size:
369 136 29 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 526 549
Size: 421 32 73 23
Fragments arranged by size:
421 73 32 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 549
Size: 386 163
MslI CAynn'nnrTG
Cuts at: 0 287 549
Size: 287 262
NaeI GCC'GGC
Cuts at: 0 189 549
Size: 189 360
NgoMIV G'CCGG_C
Cuts at: 0 187 549
Size: 187 362
NheI G'CTAG_C
Cuts at: 0 495 549
Size: 495 54
NspI r_CATG'y
Cuts at: 0 503 549
Size: 503 46
PasI CC'CwG_GG
Cuts at: 0 368 549
Size: 368 181
PfoI T'CCnGG_A
Cuts at: 0 476 549
Size: 476 73
PpuMI rG'GwC_Cy
Cuts at: 0 165 534 549
Size: 165 369 15
Fragments arranged by size:
369 165 15
PshAI GACnn'nnGTC
Cuts at: 0 170 549
Size: 170 379
PspOMI G'GGCC_C
Cuts at: 0 28 549
Size: 28 521
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 497 549
Size: 497 52
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 369 549
Size: 369 180
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI BanI BanII BglI
Bme1580I BmtI BpmI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstXI
BtgI BtsI EaeI EagI Eco57MI EcoO109I Hin4I MmeI
MslI NaeI NgoMIV NheI NspI PasI PfoI PpuMI
PshAI PspOMI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BplI
Bpu10I BpuEI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI MspA1I NarI NcoI
NdeI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI