(Linear) (Six-base) MAPSORT of: KIBB4286.seq  Check: 2026  from: 1  to: 705



KIBB4286.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     279     480     705 
   Size:        139     140     201     225

  Fragments arranged by size: 

                 225     201     140     139
 
ApoI r'AATT_y

Cuts at:      0     450     705 
   Size:        450     255
 
BamHI G'GATC_C

Cuts at:      0     686     705 
   Size:        686      19
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     160     705 
   Size:        160     545
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     705 
   Size:        325     380
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     370     373     705 
   Size:        370       3     332

  Fragments arranged by size: 

                 370     332       3
 
BseYI C'CCAG_C

Cuts at:      0     192     622     705 
   Size:        192     430      83

  Fragments arranged by size: 

                 430     192      83
 
BsiHKAI G_wGCw'C

Cuts at:      0     172     427     705 
   Size:        172     255     278

  Fragments arranged by size: 

                 278     255     172
 
BsmI GAATG_Cn'

Cuts at:      0     267     705 
   Size:        267     438
 
Bsp1286I G_dGCh'C

Cuts at:      0     172     427     705 
   Size:        172     255     278

  Fragments arranged by size: 

                 278     255     172
 
BspHI T'CATG_A

Cuts at:      0     176     705 
   Size:        176     529
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     640     705 
   Size:        640      65
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     314     471     705 
   Size:        314     157     234

  Fragments arranged by size: 

                 314     234     157
 
BstYI r'GATC_y

Cuts at:      0     686     705 
   Size:        686      19
 
BtgI C'CryG_G

Cuts at:      0     644     705 
   Size:        644      61
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     240     705 
   Size:        240     465
 
EaeI y'GGCC_r

Cuts at:      0      18     604     705 
   Size:         18     586     101

  Fragments arranged by size: 

                 586     101      18
 
EarI CTCTTCn'nnn_

Cuts at:      0      33     233     392     541     604     705 
   Size:         33     200     159     149      63     101

  Fragments arranged by size: 

                 200     159     149     101      63      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     279     325     480     705 
   Size:        139     140      46     155     225

  Fragments arranged by size: 

                 225     155     140     139      46
 
HincII GTy'rAC

Cuts at:      0     354     705 
   Size:        354     351
 
HpaI GTT'AAC

Cuts at:      0     354     705 
   Size:        354     351
 
MfeI C'AATT_G

Cuts at:      0     635     705 
   Size:        635      70
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     602     705 
   Size:        602     103
 
MscI TGG'CCA

Cuts at:      0     606     705 
   Size:        606      99
 
MslI CAynn'nnrTG

Cuts at:      0     178     705 
   Size:        178     527
 
MspA1I CmG'CkG

Cuts at:      0     299     705 
   Size:        299     406
 
NcoI C'CATG_G

Cuts at:      0     644     705 
   Size:        644      61
 
NspI r_CATG'y

Cuts at:      0     222     705 
   Size:        222     483
 
PfoI T'CCnGG_A

Cuts at:      0      89     705 
   Size:         89     616
 
PvuII CAG'CTG

Cuts at:      0     299     705 
   Size:        299     406
 
SfcI C'TryA_G

Cuts at:      0     570     705 
   Size:        570     135
 
SspI AAT'ATT

Cuts at:      0      60     705 
   Size:         60     645
 
StyI C'CwwG_G

Cuts at:      0     644     705 
   Size:        644      61
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     149     250     705 
   Size:        149     101     455

  Fragments arranged by size: 

                 455     149     101
 

 Enzymes that do cut:

     AcuI     ApoI    BamHI     BplI     BpmI    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BspHI   BstAPI    BstXI    BstYI     BtgI     DrdI
     EaeI     EarI  Eco57MI   HincII     HpaI     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NspI     PfoI    PvuII     SfcI     SspI
     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     KasI     KpnI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI