Restriction map for
pF1KB4274
(Linear) (Six-base) MAPSORT of: KIBB4274.seq Check: 5852 from: 1 to: 1941
KIBB4274.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AccI GT'mk_AC
Cuts at: 0 687 1941
Size: 687 1254
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 557 1020 1941
Size: 260 297 463 921
Fragments arranged by size:
921 463 297 260
AleI CACnn'nnGTG
Cuts at: 0 1835 1941
Size: 1835 106
AlwNI CAG_nnn'CTG
Cuts at: 0 66 1941
Size: 66 1875
ApoI r'AATT_y
Cuts at: 0 256 375 818 1574 1941
Size: 256 119 443 756 367
Fragments arranged by size:
756 443 367 256 119
AseI AT'TA_AT
Cuts at: 0 439 1941
Size: 439 1502
BbsI GAAGACnn'nnnn_
Cuts at: 0 88 248 1135 1303 1387 1807 1941
Size: 88 160 887 168 84 420 134
Fragments arranged by size:
887 420 168 160 134 88 84
BclI T'GATC_A
Cuts at: 0 891 975 1479 1941
Size: 891 84 504 462
Fragments arranged by size:
891 504 462 84
BfrBI ATG'CAT
Cuts at: 0 865 1941
Size: 865 1076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1941
Size: 599 1342
Bpu10I CC'TnA_GC
Cuts at: 0 5 1941
Size: 5 1936
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 943 1941
Size: 151 792 998
Fragments arranged by size:
998 792 151
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 597 627 991 1021 1941
Size: 597 30 364 30 920
Fragments arranged by size:
920 597 364 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 194 1348 1941
Size: 67 127 1154 593
Fragments arranged by size:
1154 593 127 67
BsmI GAATG_Cn'
Cuts at: 0 1035 1941
Size: 1035 906
BspHI T'CATG_A
Cuts at: 0 1314 1941
Size: 1314 627
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 196 223 1941
Size: 196 27 1718
Fragments arranged by size:
1718 196 27
BsrDI GCAATG_nn'
Cuts at: 0 771 939 1027 1941
Size: 771 168 88 914
Fragments arranged by size:
914 771 168 88
BsrFI r'CCGG_y
Cuts at: 0 1666 1941
Size: 1666 275
BsrGI T'GTAC_A
Cuts at: 0 1227 1647 1815 1941
Size: 1227 420 168 126
Fragments arranged by size:
1227 420 168 126
BtgI C'CryG_G
Cuts at: 0 190 1941
Size: 190 1751
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 753 788 921 956 1941
Size: 753 35 133 35 985
Fragments arranged by size:
985 753 133 35 35
EaeI y'GGCC_r
Cuts at: 0 1668 1941
Size: 1668 273
EarI CTCTTCn'nnn_
Cuts at: 0 179 322 726 1316 1941
Size: 179 143 404 590 625
Fragments arranged by size:
625 590 404 179 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 943 1111 1363 1783 1941
Size: 943 168 252 420 158
Fragments arranged by size:
943 420 252 168 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 557 599 1020 1941
Size: 260 297 42 421 921
Fragments arranged by size:
921 421 297 260 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 695 1710 1941
Size: 695 1015 231
Fragments arranged by size:
1015 695 231
EcoRI G'AATT_C
Cuts at: 0 375 818 1574 1941
Size: 375 443 756 367
Fragments arranged by size:
756 443 375 367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 339 371 516 548 795 827 1941
Size: 339 32 145 32 247 32 1114
Fragments arranged by size:
1114 339 247 145 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 152 1941
Size: 86 66 1789
Fragments arranged by size:
1789 86 66
MslI CAynn'nnrTG
Cuts at: 0 130 854 1095 1163 1515 1767 1835 1933
Size: 130 724 241 68 352 252 68 98
Cuts at: 1933 1941
Size: 8
Fragments arranged by size:
724 352 252 241 130 98 68 68
8
NcoI C'CATG_G
Cuts at: 0 190 1941
Size: 190 1751
NdeI CA'TA_TG
Cuts at: 0 1092 1512 1941
Size: 1092 420 429
Fragments arranged by size:
1092 429 420
NsiI A_TGCA'T
Cuts at: 0 867 1941
Size: 867 1074
NspI r_CATG'y
Cuts at: 0 764 1941
Size: 764 1177
PsiI TTA'TAA
Cuts at: 0 1883 1941
Size: 1883 58
PstI C_TGCA'G
Cuts at: 0 790 1941
Size: 790 1151
SapI GCTCTTCn'nnn_
Cuts at: 0 179 726 1316 1941
Size: 179 547 590 625
Fragments arranged by size:
625 590 547 179
ScaI AGT'ACT
Cuts at: 0 513 1941
Size: 513 1428
SfcI C'TryA_G
Cuts at: 0 786 1941
Size: 786 1155
SmlI C'TyrA_G
Cuts at: 0 166 958 1941
Size: 166 792 983
Fragments arranged by size:
983 792 166
SspI AAT'ATT
Cuts at: 0 744 1941
Size: 744 1197
StuI AGG'CCT
Cuts at: 0 671 1453 1941
Size: 671 782 488
Fragments arranged by size:
782 671 488
StyI C'CwwG_G
Cuts at: 0 190 1941
Size: 190 1751
TatI w'GTAC_w
Cuts at: 0 511 1227 1647 1815 1941
Size: 511 716 420 168 126
Fragments arranged by size:
716 511 420 168 126
XbaI T'CTAG_A
Cuts at: 0 283 1941
Size: 283 1658
XmnI GAAnn'nnTTC
Cuts at: 0 652 781 1033 1117 1285 1941
Size: 652 129 252 84 168 656
Fragments arranged by size:
656 652 252 168 129 84
Enzymes that do cut:
AccI AcuI AleI AlwNI ApoI AseI BbsI BclI
BfrBI BpmI Bpu10I BpuEI BsaXI BseRI BsmI BspHI
BspMI BsrDI BsrFI BsrGI BtgI CspCI EaeI EarI
EciI Eco57MI EcoNI EcoRI Hin4I MmeI MslI NcoI
NdeI NsiI NspI PsiI PstI SapI ScaI SfcI
SmlI SspI StuI StyI TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI DraI DraIII DrdI EagI EcoICRI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TaqII TstI
Tth111I XcmI XhoI XmaI ZraI