Restriction map for
pF1KB4270
(Linear) (Six-base) MAPSORT of: KIBB4270.seq Check: 5736 from: 1 to: 1257
KIBB4270.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 860 1257
Size: 223 637 397
Fragments arranged by size:
637 397 223
AflIII A'CryG_T
Cuts at: 0 380 928 1257
Size: 380 548 329
Fragments arranged by size:
548 380 329
ApaI G_GGCC'C
Cuts at: 0 984 1257
Size: 984 273
ApoI r'AATT_y
Cuts at: 0 451 1129 1257
Size: 451 678 128
Fragments arranged by size:
678 451 128
AvaI C'yCGr_G
Cuts at: 0 671 1257
Size: 671 586
BamHI G'GATC_C
Cuts at: 0 824 1257
Size: 824 433
BanII G_rGCy'C
Cuts at: 0 678 984 1257
Size: 678 306 273
Fragments arranged by size:
678 306 273
BbsI GAAGACnn'nnnn_
Cuts at: 0 957 1257
Size: 957 300
BbvCI CC'TCA_GC
Cuts at: 0 853 1257
Size: 853 404
BciVI GTATCCnnnnn_n'
Cuts at: 0 1202 1257
Size: 1202 55
Bme1580I G_kGCm'C
Cuts at: 0 200 984 1257
Size: 200 784 273
Fragments arranged by size:
784 273 200
BmrI ACTGGGnnnn_n'
Cuts at: 0 675 880 1257
Size: 675 205 377
Fragments arranged by size:
675 377 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1137 1257
Size: 1137 120
Bpu10I CC'TnA_GC
Cuts at: 0 584 853 1257
Size: 584 269 404
Fragments arranged by size:
584 404 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 1069 1257
Size: 840 229 188
Fragments arranged by size:
840 229 188
BsaI GGTCTCn'nnnn_
Cuts at: 0 880 885 1257
Size: 880 5 372
Fragments arranged by size:
880 372 5
BsaAI yAC'GTr
Cuts at: 0 931 1257
Size: 931 326
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 78 108 138 168 1257
Size: 78 30 30 30 1089
Fragments arranged by size:
1089 78 30 30 30
BseYI C'CCAG_C
Cuts at: 0 977 1257
Size: 977 280
BsiHKAI G_wGCw'C
Cuts at: 0 678 1257
Size: 678 579
Bsp1286I G_dGCh'C
Cuts at: 0 200 678 984 1257
Size: 200 478 306 273
Fragments arranged by size:
478 306 273 200
BspHI T'CATG_A
Cuts at: 0 878 1257
Size: 878 379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 665 1257
Size: 665 592
BsrDI GCAATG_nn'
Cuts at: 0 124 1257
Size: 124 1133
BstXI CCAn_nnnn'nTGG
Cuts at: 0 870 1257
Size: 870 387
BstYI r'GATC_y
Cuts at: 0 824 1257
Size: 824 433
BtgI C'CryG_G
Cuts at: 0 149 1257
Size: 149 1108
BtsI GCAGTG_nn'
Cuts at: 0 20 398 1257
Size: 20 378 859
Fragments arranged by size:
859 378 20
DraI TTT'AAA
Cuts at: 0 924 1257
Size: 924 333
DrdI GACnn_nn'nnGTC
Cuts at: 0 1211 1257
Size: 1211 46
EarI CTCTTCn'nnn_
Cuts at: 0 242 1257
Size: 242 1015
EcoICRI GAG'CTC
Cuts at: 0 676 1257
Size: 676 581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 860 1137 1257
Size: 223 637 277 120
Fragments arranged by size:
637 277 223 120
EcoNI CCTnn'n_nnAGG
Cuts at: 0 856 1257
Size: 856 401
EcoO109I rG'GnC_Cy
Cuts at: 0 981 1257
Size: 981 276
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 494 526 1257
Size: 494 32 731
Fragments arranged by size:
731 494 32
HincII GTy'rAC
Cuts at: 0 20 1257
Size: 20 1237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 1257
Size: 68 1189
MslI CAynn'nnrTG
Cuts at: 0 76 913 1257
Size: 76 837 344
Fragments arranged by size:
837 344 76
MspA1I CmG'CkG
Cuts at: 0 193 1104 1257
Size: 193 911 153
Fragments arranged by size:
911 193 153
NcoI C'CATG_G
Cuts at: 0 149 1257
Size: 149 1108
NspI r_CATG'y
Cuts at: 0 384 405 1257
Size: 384 21 852
Fragments arranged by size:
852 384 21
PciI A'CATG_T
Cuts at: 0 380 1257
Size: 380 877
PmlI CAC'GTG
Cuts at: 0 931 1257
Size: 931 326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 572 604 1257
Size: 572 32 653
Fragments arranged by size:
653 572 32
PshAI GACnn'nnGTC
Cuts at: 0 119 1257
Size: 119 1138
PspOMI G'GGCC_C
Cuts at: 0 980 1257
Size: 980 277
PstI C_TGCA'G
Cuts at: 0 662 890 1257
Size: 662 228 367
Fragments arranged by size:
662 367 228
PvuII CAG'CTG
Cuts at: 0 193 1257
Size: 193 1064
SacI G_AGCT'C
Cuts at: 0 678 1257
Size: 678 579
SfcI C'TryA_G
Cuts at: 0 459 658 886 1257
Size: 459 199 228 371
Fragments arranged by size:
459 371 228 199
SmlI C'TyrA_G
Cuts at: 0 819 1084 1257
Size: 819 265 173
Fragments arranged by size:
819 265 173
SspI AAT'ATT
Cuts at: 0 84 1257
Size: 84 1173
StyI C'CwwG_G
Cuts at: 0 149 1257
Size: 149 1108
TatI w'GTAC_w
Cuts at: 0 187 776 1257
Size: 187 589 481
Fragments arranged by size:
589 481 187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 690 722 1257
Size: 690 32 535
Fragments arranged by size:
690 535 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 688 1257
Size: 688 569
XmnI GAAnn'nnTTC
Cuts at: 0 454 1257
Size: 454 803
Enzymes that do cut:
AcuI AflIII ApaI ApoI AvaI BamHI BanII BbsI
BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaXI BseYI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BstXI BstYI BtgI BtsI DraI DrdI EarI EcoICRI
Eco57MI EcoNI EcoO109I Hin4I HincII MmeI MslI MspA1I
NcoI NspI PciI PmlI PpiI PshAI PspOMI PstI
PvuII SacI SfcI SmlI SspI StyI TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BbeI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BsaBI BsaHI
BsaWI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EaeI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PpuMI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI ZraI