Restriction map for
pF1KB4251
(Linear) (Six-base) MAPSORT of: KIBB4251.seq Check: 6834 from: 1 to: 2919
KIBB4251.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 138 469 2919
Size: 138 331 2450
Fragments arranged by size:
2450 331 138
AccI GT'mk_AC
Cuts at: 0 2415 2919
Size: 2415 504
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1515 2140 2383 2485 2919
Size: 504 1011 625 243 102 434
Fragments arranged by size:
1011 625 504 434 243 102
AflIII A'CryG_T
Cuts at: 0 1695 1868 2786 2919
Size: 1695 173 918 133
Fragments arranged by size:
1695 918 173 133
AleI CACnn'nnGTG
Cuts at: 0 1586 2919
Size: 1586 1333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1631 2919
Size: 1631 1288
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2873 2905 2919
Size: 2873 32 14
Fragments arranged by size:
2873 32 14
AlwNI CAG_nnn'CTG
Cuts at: 0 2766 2919
Size: 2766 153
ApaI G_GGCC'C
Cuts at: 0 2026 2919
Size: 2026 893
AvaI C'yCGr_G
Cuts at: 0 533 1853 2318 2919
Size: 533 1320 465 601
Fragments arranged by size:
1320 601 533 465
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1257 1290 2919
Size: 1257 33 1629
Fragments arranged by size:
1629 1257 33
BamHI G'GATC_C
Cuts at: 0 3 363 2919
Size: 3 360 2556
Fragments arranged by size:
2556 360 3
BanI G'GyrC_C
Cuts at: 0 2806 2919
Size: 2806 113
BanII G_rGCy'C
Cuts at: 0 941 1121 1573 1661 1912 2026 2186 2383
Size: 941 180 452 88 251 114 160 197
Cuts at: 2383 2749 2919
Size: 366 170
Fragments arranged by size:
941 452 366 251 197 180 170 160
114 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 2473 2919
Size: 2473 446
BbvCI CC'TCA_GC
Cuts at: 0 1985 2919
Size: 1985 934
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 753 787 2919
Size: 753 34 2132
Fragments arranged by size:
2132 753 34
BclI T'GATC_A
Cuts at: 0 2751 2919
Size: 2751 168
BfrBI ATG'CAT
Cuts at: 0 1182 1809 2919
Size: 1182 627 1110
Fragments arranged by size:
1182 1110 627
BglI GCCn_nnn'nGGC
Cuts at: 0 1895 2124 2378 2919
Size: 1895 229 254 541
Fragments arranged by size:
1895 541 254 229
BglII A'GATC_T
Cuts at: 0 1073 2919
Size: 1073 1846
BlpI GC'TnA_GC
Cuts at: 0 1553 2919
Size: 1553 1366
Bme1580I G_kGCm'C
Cuts at: 0 1821 2026 2084 2919
Size: 1821 205 58 835
Fragments arranged by size:
1821 835 205 58
BmgBI CAC'GTC
Cuts at: 0 1696 2919
Size: 1696 1223
BmrI ACTGGGnnnn_n'
Cuts at: 0 45 475 567 1421 1770 2071 2243 2919
Size: 45 430 92 854 349 301 172 676
Fragments arranged by size:
854 676 430 349 301 172 92 45
BmtI G_CTAG'C
Cuts at: 0 1097 1132 2851 2919
Size: 1097 35 1719 68
Fragments arranged by size:
1719 1097 68 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 363 960 2919
Size: 363 597 1959
Fragments arranged by size:
1959 597 363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 955 2919
Size: 575 380 1964
Fragments arranged by size:
1964 575 380
Bpu10I CC'TnA_GC
Cuts at: 0 1484 1985 2743 2919
Size: 1484 501 758 176
Fragments arranged by size:
1484 758 501 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 2919
Size: 886 2033
BsaI GGTCTCn'nnnn_
Cuts at: 0 219 397 1775 2919
Size: 219 178 1378 1144
Fragments arranged by size:
1378 1144 219 178
BsaAI yAC'GTr
Cuts at: 0 2029 2919
Size: 2029 890
BsaBI GATnn'nnATC
Cuts at: 0 2666 2919
Size: 2666 253
BsaWI w'CCGG_w
Cuts at: 0 1501 2919
Size: 1501 1418
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2598 2628 2919
Size: 2598 30 291
Fragments arranged by size:
2598 291 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2582 2919
Size: 2582 337
BseYI C'CCAG_C
Cuts at: 0 108 382 2919
Size: 108 274 2537
Fragments arranged by size:
2537 274 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 2089 2919
Size: 568 1521 830
Fragments arranged by size:
1521 830 568
BsiHKAI G_wGCw'C
Cuts at: 0 129 2186 2919
Size: 129 2057 733
Fragments arranged by size:
2057 733 129
BsmI GAATG_Cn'
Cuts at: 0 956 1603 2702 2919
Size: 956 647 1099 217
Fragments arranged by size:
1099 956 647 217
Bsp1286I G_dGCh'C
Cuts at: 0 129 941 1121 1573 1661 1821 1912 2026
Size: 129 812 180 452 88 160 91 114
Cuts at: 2026 2084 2186 2383 2749 2919
Size: 58 102 197 366 170
Fragments arranged by size:
812 452 366 197 180 170 160 129
114 102 91 88 58
BspHI T'CATG_A
Cuts at: 0 2877 2919
Size: 2877 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 138 469 2919
Size: 138 331 2450
Fragments arranged by size:
2450 331 138
BsrDI GCAATG_nn'
Cuts at: 0 1408 1847 2919
Size: 1408 439 1072
Fragments arranged by size:
1408 1072 439
BsrFI r'CCGG_y
Cuts at: 0 802 1885 1914 2919
Size: 802 1083 29 1005
Fragments arranged by size:
1083 1005 802 29
BsrGI T'GTAC_A
Cuts at: 0 1677 2919
Size: 1677 1242
BssSI C'ACGA_G
Cuts at: 0 2374 2919
Size: 2374 545
BstBI TT'CG_AA
Cuts at: 0 2103 2919
Size: 2103 816
BstXI CCAn_nnnn'nTGG
Cuts at: 0 80 151 1919 2541 2919
Size: 80 71 1768 622 378
Fragments arranged by size:
1768 622 378 80 71
BstYI r'GATC_y
Cuts at: 0 3 363 826 929 1073 2919
Size: 3 360 463 103 144 1846
Fragments arranged by size:
1846 463 360 144 103 3
BtgI C'CryG_G
Cuts at: 0 2267 2919
Size: 2267 652
BtsI GCAGTG_nn'
Cuts at: 0 812 2919
Size: 812 2107
EaeI y'GGCC_r
Cuts at: 0 82 1078 2457 2919
Size: 82 996 1379 462
Fragments arranged by size:
1379 996 462 82
EarI CTCTTCn'nnn_
Cuts at: 0 429 465 1126 1391 2919
Size: 429 36 661 265 1528
Fragments arranged by size:
1528 661 429 265 36
EcoICRI GAG'CTC
Cuts at: 0 2184 2919
Size: 2184 735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 575 955 1515 2140 2383 2485 2919
Size: 504 71 380 560 625 243 102 434
Fragments arranged by size:
625 560 504 434 380 243 102 71
EcoO109I rG'GnC_Cy
Cuts at: 0 538 2919
Size: 538 2381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1338 1545 2919
Size: 1338 207 1374
Fragments arranged by size:
1374 1338 207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 671 703 818 850 1058 1090 1847 1879
Size: 671 32 115 32 208 32 757 32
Cuts at: 1879 2325 2357 2434 2466 2736 2768 2919
Size: 446 32 77 32 270 32 151
Fragments arranged by size:
757 671 446 270 208 151 115 77
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1866 2919
Size: 1866 1053
HindIII A'AGCT_T
Cuts at: 0 89 850 1009 1231 2513 2919
Size: 89 761 159 222 1282 406
Fragments arranged by size:
1282 761 406 222 159 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 397 1853 2919
Size: 36 361 1456 1066
Fragments arranged by size:
1456 1066 361 36
MscI TGG'CCA
Cuts at: 0 84 1080 2459 2919
Size: 84 996 1379 460
Fragments arranged by size:
1379 996 460 84
MslI CAynn'nnrTG
Cuts at: 0 51 1586 1897 1903 2017 2919
Size: 51 1535 311 6 114 902
Fragments arranged by size:
1535 902 311 114 51 6
MspA1I CmG'CkG
Cuts at: 0 549 2895 2919
Size: 549 2346 24
Fragments arranged by size:
2346 549 24
NaeI GCC'GGC
Cuts at: 0 1887 2919
Size: 1887 1032
NdeI CA'TA_TG
Cuts at: 0 2756 2919
Size: 2756 163
NgoMIV G'CCGG_C
Cuts at: 0 1885 2919
Size: 1885 1034
NheI G'CTAG_C
Cuts at: 0 1093 1128 2847 2919
Size: 1093 35 1719 72
Fragments arranged by size:
1719 1093 72 35
NsiI A_TGCA'T
Cuts at: 0 1184 1811 2919
Size: 1184 627 1108
Fragments arranged by size:
1184 1108 627
NspI r_CATG'y
Cuts at: 0 780 1186 1872 1902 2262 2790 2919
Size: 780 406 686 30 360 528 129
Fragments arranged by size:
780 686 528 406 360 129 30
PciI A'CATG_T
Cuts at: 0 1868 2786 2919
Size: 1868 918 133
Fragments arranged by size:
1868 918 133
PflMI CCAn_nnn'nTGG
Cuts at: 0 359 2919
Size: 359 2560
PfoI T'CCnGG_A
Cuts at: 0 932 1214 2616 2919
Size: 932 282 1402 303
Fragments arranged by size:
1402 932 303 282
PmlI CAC'GTG
Cuts at: 0 2029 2919
Size: 2029 890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2893 2919
Size: 2893 26
PpuMI rG'GwC_Cy
Cuts at: 0 538 2919
Size: 538 2381
PshAI GACnn'nnGTC
Cuts at: 0 1954 2919
Size: 1954 965
PspOMI G'GGCC_C
Cuts at: 0 2022 2919
Size: 2022 897
PstI C_TGCA'G
Cuts at: 0 135 2919
Size: 135 2784
PvuII CAG'CTG
Cuts at: 0 549 2895 2919
Size: 549 2346 24
Fragments arranged by size:
2346 549 24
SacI G_AGCT'C
Cuts at: 0 2186 2919
Size: 2186 733
SanDI GG'GwC_CC
Cuts at: 0 538 2919
Size: 538 2381
SapI GCTCTTCn'nnn_
Cuts at: 0 465 1126 2919
Size: 465 661 1793
Fragments arranged by size:
1793 661 465
SbfI CC_TGCA'GG
Cuts at: 0 135 2919
Size: 135 2784
ScaI AGT'ACT
Cuts at: 0 2571 2919
Size: 2571 348
SfcI C'TryA_G
Cuts at: 0 131 1054 1365 2497 2919
Size: 131 923 311 1132 422
Fragments arranged by size:
1132 923 422 311 131
SmaI CCC'GGG
Cuts at: 0 535 2320 2919
Size: 535 1785 599
Fragments arranged by size:
1785 599 535
SmlI C'TyrA_G
Cuts at: 0 865 1853 2919
Size: 865 988 1066
Fragments arranged by size:
1066 988 865
SphI G_CATG'C
Cuts at: 0 1186 1902 2262 2919
Size: 1186 716 360 657
Fragments arranged by size:
1186 716 657 360
SspI AAT'ATT
Cuts at: 0 1248 2919
Size: 1248 1671
StuI AGG'CCT
Cuts at: 0 230 2919
Size: 230 2689
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 59 1955 2164 2646 2919
Size: 59 1896 209 482 273
Fragments arranged by size:
1896 482 273 209 59
TatI w'GTAC_w
Cuts at: 0 1225 1677 2569 2789 2919
Size: 1225 452 892 220 130
Fragments arranged by size:
1225 892 452 220 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1149 1181 2919
Size: 1149 32 1738
Fragments arranged by size:
1738 1149 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1545 2919
Size: 1545 1374
XhoI C'TCGA_G
Cuts at: 0 1853 2919
Size: 1853 1066
XmaI C'CCGG_G
Cuts at: 0 533 2318 2919
Size: 533 1785 601
Fragments arranged by size:
1785 601 533
XmnI GAAnn'nnTTC
Cuts at: 0 275 1528 2908 2919
Size: 275 1253 1380 11
Fragments arranged by size:
1380 1253 275 11
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AloI AlwNI
ApaI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstBI
BstXI BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI
EcoO109I FalI Hin4I HincII HindIII MmeI MscI MslI
MspA1I NaeI NdeI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuII SacI SanDI SapI SbfI ScaI SfcI SmaI
SmlI SphI SspI StuI TaqII TatI TstI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI ApaLI
ApoI AscI AseI AsiSI AvrII BbeI BciVI BsaHI
BsiEI BsiWI BsmBI BspEI BsrBI BssHII BstAPI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI NarI NcoI
NotI NruI PacI PasI PmeI PsiI PspXI PsrI
PvuI RsrII SacII SalI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI StyI SwaI Tth111I XbaI
ZraI