Restriction map for
pF1KB4190
(Linear) (Six-base) MAPSORT of: KIBB4190.seq Check: 3576 from: 1 to: 1092
KIBB4190.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 526 1092
Size: 526 566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 739 1092
Size: 37 702 353
Fragments arranged by size:
702 353 37
AfeI AGC'GCT
Cuts at: 0 276 1092
Size: 276 816
AleI CACnn'nnGTG
Cuts at: 0 652 1092
Size: 652 440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 367 1092
Size: 367 725
AlwNI CAG_nnn'CTG
Cuts at: 0 243 680 762 1025 1092
Size: 243 437 82 263 67
Fragments arranged by size:
437 263 243 82 67
ApaI G_GGCC'C
Cuts at: 0 12 1092
Size: 12 1080
AvaI C'yCGr_G
Cuts at: 0 745 1092
Size: 745 347
BamHI G'GATC_C
Cuts at: 0 29 1092
Size: 29 1063
BanI G'GyrC_C
Cuts at: 0 298 574 592 683 1092
Size: 298 276 18 91 409
Fragments arranged by size:
409 298 276 91 18
BanII G_rGCy'C
Cuts at: 0 12 1055 1092
Size: 12 1043 37
Fragments arranged by size:
1043 37 12
BbeI G_GCGC'C
Cuts at: 0 687 1092
Size: 687 405
BbsI GAAGACnn'nnnn_
Cuts at: 0 360 447 591 1045 1092
Size: 360 87 144 454 47
Fragments arranged by size:
454 360 144 87 47
BbvCI CC'TCA_GC
Cuts at: 0 271 1092
Size: 271 821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 582 616 1092
Size: 582 34 476
Fragments arranged by size:
582 476 34
BclI T'GATC_A
Cuts at: 0 380 1092
Size: 380 712
BglI GCCn_nnn'nGGC
Cuts at: 0 827 1092
Size: 827 265
Bme1580I G_kGCm'C
Cuts at: 0 12 595 792 901 918 1092
Size: 12 583 197 109 17 174
Fragments arranged by size:
583 197 174 109 17 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 1092
Size: 153 939
BmtI G_CTAG'C
Cuts at: 0 617 1092
Size: 617 475
Bpu10I CC'TnA_GC
Cuts at: 0 271 1092
Size: 271 821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 469 1092
Size: 388 81 623
Fragments arranged by size:
623 388 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 138 943 1092
Size: 138 805 149
Fragments arranged by size:
805 149 138
BsaAI yAC'GTr
Cuts at: 0 846 1092
Size: 846 246
BsaHI Gr'CG_yC
Cuts at: 0 684 1092
Size: 684 408
BsaWI w'CCGG_w
Cuts at: 0 1044 1092
Size: 1044 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 181 211 1092
Size: 181 30 881
Fragments arranged by size:
881 181 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 961 990 1063 1092
Size: 961 29 73 29
Fragments arranged by size:
961 73 29 29
BseYI C'CCAG_C
Cuts at: 0 313 588 776 780 813 990 1025 1054
Size: 313 275 188 4 33 177 35 29
Cuts at: 1054 1092
Size: 38
Fragments arranged by size:
313 275 188 177 38 35 33 29
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 1092
Size: 19 1073
BsiHKAI G_wGCw'C
Cuts at: 0 659 938 1092
Size: 659 279 154
Fragments arranged by size:
659 279 154
Bsp1286I G_dGCh'C
Cuts at: 0 12 595 659 792 901 918 938 1055
Size: 12 583 64 133 109 17 20 117
Cuts at: 1055 1092
Size: 37
Fragments arranged by size:
583 133 117 109 64 37 20 17
12
BspEI T'CCGG_A
Cuts at: 0 1044 1092
Size: 1044 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 191 485 526 1092
Size: 191 294 41 566
Fragments arranged by size:
566 294 191 41
BsrBI CCG'CTC
Cuts at: 0 440 1092
Size: 440 652
BsrDI GCAATG_nn'
Cuts at: 0 112 1092
Size: 112 980
BstEII G'GTnAC_C
Cuts at: 0 147 246 1092
Size: 147 99 846
Fragments arranged by size:
846 147 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 162 1092
Size: 162 930
BstYI r'GATC_y
Cuts at: 0 29 1092
Size: 29 1063
Bsu36I CC'TnA_GG
Cuts at: 0 331 1092
Size: 331 761
BtgI C'CryG_G
Cuts at: 0 161 302 1082 1092
Size: 161 141 780 10
Fragments arranged by size:
780 161 141 10
BtsI GCAGTG_nn'
Cuts at: 0 792 1092
Size: 792 300
DraIII CAC_nnn'GTG
Cuts at: 0 891 1092
Size: 891 201
EaeI y'GGCC_r
Cuts at: 0 281 902 929 1092
Size: 281 621 27 163
Fragments arranged by size:
621 281 163 27
EarI CTCTTCn'nnn_
Cuts at: 0 727 1069 1092
Size: 727 342 23
Fragments arranged by size:
727 342 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 993 1092
Size: 993 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 739 1092
Size: 37 702 353
Fragments arranged by size:
702 353 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1017 1092
Size: 1017 75
EcoO109I rG'GnC_Cy
Cuts at: 0 8 9 53 759 1092
Size: 8 1 44 706 333
Fragments arranged by size:
706 333 44 8 1
HaeII r_GCGC'y
Cuts at: 0 46 278 687 1082 1092
Size: 46 232 409 395 10
Fragments arranged by size:
409 395 232 46 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 845 877 1092
Size: 181 32 632 32 215
Fragments arranged by size:
632 215 181 32 32
HincII GTy'rAC
Cuts at: 0 708 1092
Size: 708 384
KasI G'GCGC_C
Cuts at: 0 683 1092
Size: 683 409
MslI CAynn'nnrTG
Cuts at: 0 160 376 652 985 1087 1092
Size: 160 216 276 333 102 5
Fragments arranged by size:
333 276 216 160 102 5
MspA1I CmG'CkG
Cuts at: 0 780 879 1092
Size: 780 99 213
Fragments arranged by size:
780 213 99
NarI GG'CG_CC
Cuts at: 0 684 1092
Size: 684 408
NcoI C'CATG_G
Cuts at: 0 161 1082 1092
Size: 161 921 10
Fragments arranged by size:
921 161 10
NheI G'CTAG_C
Cuts at: 0 613 1092
Size: 613 479
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 1092
Size: 243 849
PfoI T'CCnGG_A
Cuts at: 0 25 1092
Size: 25 1067
PspOMI G'GGCC_C
Cuts at: 0 8 1092
Size: 8 1084
PstI C_TGCA'G
Cuts at: 0 72 860 1092
Size: 72 788 232
Fragments arranged by size:
788 232 72
PvuII CAG'CTG
Cuts at: 0 780 1092
Size: 780 312
SapI GCTCTTCn'nnn_
Cuts at: 0 1069 1092
Size: 1069 23
SfcI C'TryA_G
Cuts at: 0 68 856 1092
Size: 68 788 236
Fragments arranged by size:
788 236 68
SfoI GGC'GCC
Cuts at: 0 685 1092
Size: 685 407
SmlI C'TyrA_G
Cuts at: 0 403 484 1092
Size: 403 81 608
Fragments arranged by size:
608 403 81
StuI AGG'CCT
Cuts at: 0 269 910 1092
Size: 269 641 182
Fragments arranged by size:
641 269 182
StyI C'CwwG_G
Cuts at: 0 161 905 1082 1092
Size: 161 744 177 10
Fragments arranged by size:
744 177 161 10
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 1092
Size: 321 771
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BglI Bme1580I BmrI BmtI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrDI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI MslI MspA1I NarI
NcoI NheI PflMI PfoI PspOMI PstI PvuII SapI
SfcI SfoI SmlI StuI StyI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BciVI BfrBI BglII BlpI BmgBI BplI BpmI BsaBI
BsiEI BsiWI BsmI BsmBI BspHI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI
NaeI NdeI NgoMIV NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI