Restriction map for
pF1KB4185
(Linear) (Six-base) MAPSORT of: KIBB4185.seq Check: 7863 from: 1 to: 1155
KIBB4185.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 0 289 1155
Size: 289 866
AccI GT'mk_AC
Cuts at: 0 89 1155
Size: 89 1066
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1155
Size: 244 911
AlwNI CAG_nnn'CTG
Cuts at: 0 771 1155
Size: 771 384
ApoI r'AATT_y
Cuts at: 0 850 1155
Size: 850 305
AvaI C'yCGr_G
Cuts at: 0 69 1155
Size: 69 1086
BfrBI ATG'CAT
Cuts at: 0 869 1155
Size: 869 286
BlpI GC'TnA_GC
Cuts at: 0 479 962 1155
Size: 479 483 193
Fragments arranged by size:
483 479 193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 494 1155
Size: 494 661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 434 445 1155
Size: 430 4 11 710
Fragments arranged by size:
710 430 11 4
Bpu10I CC'TnA_GC
Cuts at: 0 108 1155
Size: 108 1047
BsaHI Gr'CG_yC
Cuts at: 0 257 286 1155
Size: 257 29 869
Fragments arranged by size:
869 257 29
BsaWI w'CCGG_w
Cuts at: 0 180 705 833 923 1155
Size: 180 525 128 90 232
Fragments arranged by size:
525 232 180 128 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 1155
Size: 538 30 587
Fragments arranged by size:
587 538 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 1155
Size: 349 806
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 283 1155
Size: 228 55 872
Fragments arranged by size:
872 228 55
BsiEI CG_ry'CG
Cuts at: 0 63 1155
Size: 63 1092
BsmI GAATG_Cn'
Cuts at: 0 933 1155
Size: 933 222
BspEI T'CCGG_A
Cuts at: 0 923 1155
Size: 923 232
BspHI T'CATG_A
Cuts at: 0 581 1155
Size: 581 574
BsrBI CCG'CTC
Cuts at: 0 273 456 1155
Size: 273 183 699
Fragments arranged by size:
699 273 183
BsrDI GCAATG_nn'
Cuts at: 0 938 1110 1155
Size: 938 172 45
Fragments arranged by size:
938 172 45
BsrFI r'CCGG_y
Cuts at: 0 381 1155
Size: 381 774
BsrGI T'GTAC_A
Cuts at: 0 1136 1155
Size: 1136 19
BssSI C'ACGA_G
Cuts at: 0 268 466 1155
Size: 268 198 689
Fragments arranged by size:
689 268 198
BtgI C'CryG_G
Cuts at: 0 321 395 434 542 1155
Size: 321 74 39 108 613
Fragments arranged by size:
613 321 108 74 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 36 71 1155
Size: 36 35 1084
Fragments arranged by size:
1084 36 35
DraIII CAC_nnn'GTG
Cuts at: 0 901 1155
Size: 901 254
EaeI y'GGCC_r
Cuts at: 0 431 539 1155
Size: 431 108 616
Fragments arranged by size:
616 431 108
EarI CTCTTCn'nnn_
Cuts at: 0 583 1155
Size: 583 572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 430 434 445 1155
Size: 244 186 4 11 710
Fragments arranged by size:
710 244 186 11 4
EcoO109I rG'GnC_Cy
Cuts at: 0 143 277 515 1155
Size: 143 134 238 640
Fragments arranged by size:
640 238 143 134
EcoRV GAT'ATC
Cuts at: 0 39 997 1152 1155
Size: 39 958 155 3
Fragments arranged by size:
958 155 39 3
HaeII r_GCGC'y
Cuts at: 0 373 1155
Size: 373 782
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 91 123 1155
Size: 52 32 7 32 1032
Fragments arranged by size:
1032 52 32 32 7
HincII GTy'rAC
Cuts at: 0 243 1155
Size: 243 912
MscI TGG'CCA
Cuts at: 0 541 1155
Size: 541 614
MspA1I CmG'CkG
Cuts at: 0 397 408 436 565 642 1155
Size: 397 11 28 129 77 513
Fragments arranged by size:
513 397 129 77 28 11
NaeI GCC'GGC
Cuts at: 0 383 1155
Size: 383 772
NgoMIV G'CCGG_C
Cuts at: 0 381 1155
Size: 381 774
NsiI A_TGCA'T
Cuts at: 0 871 1155
Size: 871 284
PpuMI rG'GwC_Cy
Cuts at: 0 143 515 1155
Size: 143 372 640
Fragments arranged by size:
640 372 143
PshAI GACnn'nnGTC
Cuts at: 0 148 1155
Size: 148 1007
PvuII CAG'CTG
Cuts at: 0 408 565 642 1155
Size: 408 157 77 513
Fragments arranged by size:
513 408 157 77
SacII CC_GC'GG
Cuts at: 0 398 437 1155
Size: 398 39 718
Fragments arranged by size:
718 398 39
SanDI GG'GwC_CC
Cuts at: 0 515 1155
Size: 515 640
SfcI C'TryA_G
Cuts at: 0 1070 1155
Size: 1070 85
TatI w'GTAC_w
Cuts at: 0 1136 1155
Size: 1136 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 1155
Size: 538 32 585
Fragments arranged by size:
585 538 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 508 1155
Size: 508 647
ZraI GAC'GTC
Cuts at: 0 287 1155
Size: 287 868
Enzymes that do cut:
AatII AccI AcuI AlwNI ApoI AvaI BfrBI BlpI
BplI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssSI BtgI CspCI DraIII EaeI EarI Eco57MI EcoO109I
EcoRV HaeII Hin4I HincII MscI MspA1I NaeI NgoMIV
NsiI PpuMI PshAI PvuII SacII SanDI SfcI TatI
TstI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII Bme1580I BmgBI BmrI
BmtI BpuEI BsaI BsaAI BsaBI BseYI BsiHKAI BsiWI
BsmBI Bsp1286I BspMI BssHII BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI DraI DrdI
EagI EciI EcoICRI EcoNI EcoRI FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NarI NcoI NdeI NheI NotI NruI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII Tth111I XbaI XhoI XmaI
XmnI