Restriction map for
pF1KB4178
(Linear) (Six-base) MAPSORT of: KIBB4178.seq Check: 2386 from: 1 to: 1035
KIBB4178.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1035
Size: 485 550
AflII C'TTAA_G
Cuts at: 0 706 1035
Size: 706 329
AflIII A'CryG_T
Cuts at: 0 104 1035
Size: 104 931
ApaI G_GGCC'C
Cuts at: 0 185 1035
Size: 185 850
ApoI r'AATT_y
Cuts at: 0 920 1035
Size: 920 115
BanI G'GyrC_C
Cuts at: 0 166 1035
Size: 166 869
BanII G_rGCy'C
Cuts at: 0 185 218 344 581 1035
Size: 185 33 126 237 454
Fragments arranged by size:
454 237 185 126 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 23 569 1035
Size: 23 546 466
Fragments arranged by size:
546 466 23
BglII A'GATC_T
Cuts at: 0 354 469 1035
Size: 354 115 566
Fragments arranged by size:
566 354 115
Bme1580I G_kGCm'C
Cuts at: 0 185 1035
Size: 185 850
BmgBI CAC'GTC
Cuts at: 0 105 1035
Size: 105 930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 608 1035
Size: 180 428 427
Fragments arranged by size:
428 427 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 607 619 1035
Size: 565 42 12 416
Fragments arranged by size:
565 416 42 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 470 835 1035
Size: 470 365 200
Fragments arranged by size:
470 365 200
BsiEI CG_ry'CG
Cuts at: 0 86 151 1035
Size: 86 65 884
Fragments arranged by size:
884 86 65
BsiHKAI G_wGCw'C
Cuts at: 0 344 581 656 1035
Size: 344 237 75 379
Fragments arranged by size:
379 344 237 75
Bsp1286I G_dGCh'C
Cuts at: 0 185 218 344 581 656 1035
Size: 185 33 126 237 75 379
Fragments arranged by size:
379 237 185 126 75 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 1035
Size: 131 904
BsrBI CCG'CTC
Cuts at: 0 156 1035
Size: 156 879
BsrFI r'CCGG_y
Cuts at: 0 81 146 163 788 1035
Size: 81 65 17 625 247
Fragments arranged by size:
625 247 81 65 17
BstYI r'GATC_y
Cuts at: 0 354 469 1035
Size: 354 115 566
Fragments arranged by size:
566 354 115
BtgI C'CryG_G
Cuts at: 0 248 1035
Size: 248 787
DrdI GACnn_nn'nnGTC
Cuts at: 0 25 109 1035
Size: 25 84 926
Fragments arranged by size:
926 84 25
EaeI y'GGCC_r
Cuts at: 0 83 148 790 1035
Size: 83 65 642 245
Fragments arranged by size:
642 245 83 65
EagI C'GGCC_G
Cuts at: 0 83 148 1035
Size: 83 65 887
Fragments arranged by size:
887 83 65
EarI CTCTTCn'nnn_
Cuts at: 0 43 200 586 639 681 1035
Size: 43 157 386 53 42 354
Fragments arranged by size:
386 354 157 53 43 42
EcoICRI GAG'CTC
Cuts at: 0 342 579 1035
Size: 342 237 456
Fragments arranged by size:
456 342 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 485 608 1035
Size: 180 305 123 427
Fragments arranged by size:
427 305 180 123
EcoO109I rG'GnC_Cy
Cuts at: 0 181 182 1035
Size: 181 1 853
Fragments arranged by size:
853 181 1
FseI GG_CCGG'CC
Cuts at: 0 792 1035
Size: 792 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 254 286 859 891 1035
Size: 254 32 573 32 144
Fragments arranged by size:
573 254 144 32 32
HincII GTy'rAC
Cuts at: 0 1017 1035
Size: 1017 18
HindIII A'AGCT_T
Cuts at: 0 433 583 1035
Size: 433 150 452
Fragments arranged by size:
452 433 150
MspA1I CmG'CkG
Cuts at: 0 250 1035
Size: 250 785
NaeI GCC'GGC
Cuts at: 0 83 165 790 1035
Size: 83 82 625 245
Fragments arranged by size:
625 245 83 82
NgoMIV G'CCGG_C
Cuts at: 0 81 163 788 1035
Size: 81 82 625 247
Fragments arranged by size:
625 247 82 81
PfoI T'CCnGG_A
Cuts at: 0 11 1035
Size: 11 1024
PspOMI G'GGCC_C
Cuts at: 0 181 1035
Size: 181 854
PstI C_TGCA'G
Cuts at: 0 128 786 1035
Size: 128 658 249
Fragments arranged by size:
658 249 128
SacI G_AGCT'C
Cuts at: 0 344 581 1035
Size: 344 237 454
Fragments arranged by size:
454 344 237
SacII CC_GC'GG
Cuts at: 0 251 1035
Size: 251 784
SapI GCTCTTCn'nnn_
Cuts at: 0 200 1035
Size: 200 835
SfcI C'TryA_G
Cuts at: 0 124 782 1035
Size: 124 658 253
Fragments arranged by size:
658 253 124
SmlI C'TyrA_G
Cuts at: 0 580 586 634 706 1035
Size: 580 6 48 72 329
Fragments arranged by size:
580 329 72 48 6
StyI C'CwwG_G
Cuts at: 0 860 1035
Size: 860 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 15 1035
Size: 15 1020
XbaI T'CTAG_A
Cuts at: 0 327 1035
Size: 327 708
Enzymes that do cut:
AcuI AflII AflIII ApaI ApoI BanI BanII BbsI
BglII Bme1580I BmgBI BpmI BpuEI BseRI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BstYI BtgI DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoO109I FseI Hin4I HincII
HindIII MspA1I NaeI NgoMIV PfoI PspOMI PstI SacI
SacII SapI SfcI SmlI StyI TaqII XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII EciI EcoNI EcoRI
EcoRV FalI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI