(Linear) (Six-base) MAPSORT of: KIBB4172.seq  Check: 7079  from: 1  to: 1077



KIBB4172.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AccI GT'mk_AC

Cuts at:      0     473    1077 
   Size:        473     604
 
AleI CACnn'nnGTG

Cuts at:      0     910    1077 
   Size:        910     167
 
ApoI r'AATT_y

Cuts at:      0     375    1077 
   Size:        375     702
 
AseI AT'TA_AT

Cuts at:      0     797    1077 
   Size:        797     280
 
BanI G'GyrC_C

Cuts at:      0     577     809    1077 
   Size:        577     232     268

  Fragments arranged by size: 

                 577     268     232
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     110    1077 
   Size:        110     967
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     686     880    1009    1077 
   Size:        686     194     129      68

  Fragments arranged by size: 

                 686     194     129      68
 
BglI GCCn_nnn'nGGC

Cuts at:      0     560    1077 
   Size:        560     517
 
BlpI GC'TnA_GC

Cuts at:      0     692    1077 
   Size:        692     385
 
Bme1580I G_kGCm'C

Cuts at:      0     812    1077 
   Size:        812     265
 
BmtI G_CTAG'C

Cuts at:      0     691    1007    1077 
   Size:        691     316      70

  Fragments arranged by size: 

                 691     316      70
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     773    1077 
   Size:        773     304
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1050    1077 
   Size:       1050      27
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     171     201    1019    1049    1077 
   Size:        171      30     818      30      28

  Fragments arranged by size: 

                 818     171      30      30      28
 
BseYI C'CCAG_C

Cuts at:      0     647    1077 
   Size:        647     430
 
Bsp1286I G_dGCh'C

Cuts at:      0     812    1077 
   Size:        812     265
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1032    1077 
   Size:       1032      45
 
BsrGI T'GTAC_A

Cuts at:      0     839    1077 
   Size:        839     238
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     484    1077 
   Size:        484     593
 
BtgI C'CryG_G

Cuts at:      0     581     992    1077 
   Size:        581     411      85

  Fragments arranged by size: 

                 581     411      85
 
EaeI y'GGCC_r

Cuts at:      0     561    1077 
   Size:        561     516
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     818    1077 
   Size:        818     259
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     135     189     374    1077 
   Size:        135      54     185     703

  Fragments arranged by size: 

                 703     185     135      54
 
HincII GTy'rAC

Cuts at:      0      87     129     474    1077 
   Size:         87      42     345     603

  Fragments arranged by size: 

                 603     345      87      42
 
HindIII A'AGCT_T

Cuts at:      0     205    1077 
   Size:        205     872
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     401     695    1077 
   Size:         76     325     294     382

  Fragments arranged by size: 

                 382     325     294      76
 
MscI TGG'CCA

Cuts at:      0     563    1077 
   Size:        563     514
 
MslI CAynn'nnrTG

Cuts at:      0     910    1077 
   Size:        910     167
 
MspA1I CmG'CkG

Cuts at:      0     389     651    1077 
   Size:        389     262     426

  Fragments arranged by size: 

                 426     389     262
 
NcoI C'CATG_G

Cuts at:      0     992    1077 
   Size:        992      85
 
NheI G'CTAG_C

Cuts at:      0     687    1003    1077 
   Size:        687     316      74

  Fragments arranged by size: 

                 687     316      74
 
PasI CC'CwG_GG

Cuts at:      0     805    1077 
   Size:        805     272
 
PfoI T'CCnGG_A

Cuts at:      0     678    1077 
   Size:        678     399
 
PstI C_TGCA'G

Cuts at:      0    1043    1077 
   Size:       1043      34
 
PvuII CAG'CTG

Cuts at:      0     651    1077 
   Size:        651     426
 
SalI G'TCGA_C

Cuts at:      0     472    1077 
   Size:        472     605
 
ScaI AGT'ACT

Cuts at:      0    1037    1077 
   Size:       1037      40
 
SexAI A'CCwGG_T

Cuts at:      0     476    1077 
   Size:        476     601
 
SfcI C'TryA_G

Cuts at:      0    1039    1077 
   Size:       1039      38
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     560    1077 
   Size:        560     517
 
StuI AGG'CCT

Cuts at:      0     554    1077 
   Size:        554     523
 
StyI C'CwwG_G

Cuts at:      0     269     992    1077 
   Size:        269     723      85

  Fragments arranged by size: 

                 723     269      85
 
TatI w'GTAC_w

Cuts at:      0     839    1035    1077 
   Size:        839     196      42

  Fragments arranged by size: 

                 839     196      42
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     171     203    1077 
   Size:        171      32     874

  Fragments arranged by size: 

                 874     171      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     478    1077 
   Size:        478     599
 

 Enzymes that do cut:

     AccI     AleI     ApoI     AseI     BanI     BbsI    BciVI     BglI
     BlpI Bme1580I     BmtI     BplI     BsaI    BsaXI    BseYI Bsp1286I
    BspMI    BsrGI    BstXI     BtgI     EaeI    EcoNI     FalI   HincII
  HindIII     MmeI     MscI     MslI   MspA1I     NcoI     NheI     PasI
     PfoI     PstI    PvuII     SalI     ScaI    SexAI     SfcI     SfiI
     StuI     StyI     TatI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI     BclI    BfrBI    BglII    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII    SanDI     SapI     SbfI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI