(Linear) (Six-base) MAPSORT of: KIBB4156.seq  Check: 4363  from: 1  to: 426



KIBB4156.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     238     426 
   Size:        238     188
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     380     426 
   Size:        380      46
 
BclI T'GATC_A

Cuts at:      0     264     426 
   Size:        264     162
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     426 
   Size:        328      98
 
BsaAI yAC'GTr

Cuts at:      0     104     426 
   Size:        104     322
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      93     123     426 
   Size:         93      30     303

  Fragments arranged by size: 

                 303      93      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     101     426 
   Size:        101     325
 
BstYI r'GATC_y

Cuts at:      0     234     426 
   Size:        234     192
 
EaeI y'GGCC_r

Cuts at:      0     229     426 
   Size:        229     197
 
EcoO109I rG'GnC_Cy

Cuts at:      0     316     426 
   Size:        316     110
 
MslI CAynn'nnrTG

Cuts at:      0     248     426 
   Size:        248     178
 
NdeI CA'TA_TG

Cuts at:      0     204     426 
   Size:        204     222
 
PpuMI rG'GwC_Cy

Cuts at:      0     316     426 
   Size:        316     110
 
PstI C_TGCA'G

Cuts at:      0      15     426 
   Size:         15     411
 
SfcI C'TryA_G

Cuts at:      0       5      11     426 
   Size:          5       6     415

  Fragments arranged by size: 

                 415       6       5
 
SmlI C'TyrA_G

Cuts at:      0     307     426 
   Size:        307     119
 
SnaBI TAC'GTA

Cuts at:      0     104     426 
   Size:        104     322
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      12     426 
   Size:         12     414
 

 Enzymes that do cut:

    AlwNI    BciVI     BclI    BpuEI    BsaAI    BsaXI    BseRI    BstYI
     EaeI EcoO109I     MslI     NdeI    PpuMI     PstI     SfcI     SmlI
    SnaBI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI