Restriction map for
pF1KB4124
(Linear) (Six-base) MAPSORT of: KIBB4124.seq Check: 1187 from: 1 to: 2979
KIBB4124.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 0 1489 2979
Size: 1489 1490
Acc65I G'GTAC_C
Cuts at: 0 17 2979
Size: 17 2962
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1478 2979
Size: 856 622 1501
Fragments arranged by size:
1501 856 622
AflII C'TTAA_G
Cuts at: 0 1269 2979
Size: 1269 1710
AflIII A'CryG_T
Cuts at: 0 697 2501 2979
Size: 697 1804 478
Fragments arranged by size:
1804 697 478
AleI CACnn'nnGTG
Cuts at: 0 508 749 2979
Size: 508 241 2230
Fragments arranged by size:
2230 508 241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 767 2979
Size: 767 2212
AlwNI CAG_nnn'CTG
Cuts at: 0 658 2350 2979
Size: 658 1692 629
Fragments arranged by size:
1692 658 629
ApoI r'AATT_y
Cuts at: 0 307 667 1786 1912 2375 2419 2629 2648
Size: 307 360 1119 126 463 44 210 19
Cuts at: 2648 2979
Size: 331
Fragments arranged by size:
1119 463 360 331 307 210 126 44
19
AseI AT'TA_AT
Cuts at: 0 1505 2979
Size: 1505 1474
AvaI C'yCGr_G
Cuts at: 0 2324 2979
Size: 2324 655
BanI G'GyrC_C
Cuts at: 0 17 1795 2979
Size: 17 1778 1184
Fragments arranged by size:
1778 1184 17
BanII G_rGCy'C
Cuts at: 0 236 957 2139 2979
Size: 236 721 1182 840
Fragments arranged by size:
1182 840 721 236
BbsI GAAGACnn'nnnn_
Cuts at: 0 325 848 2979
Size: 325 523 2131
Fragments arranged by size:
2131 523 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1310 1344 2979
Size: 1310 34 1635
Fragments arranged by size:
1635 1310 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1153 2979
Size: 1153 1826
BfrBI ATG'CAT
Cuts at: 0 2146 2979
Size: 2146 833
BglI GCCn_nnn'nGGC
Cuts at: 0 2573 2979
Size: 2573 406
BglII A'GATC_T
Cuts at: 0 867 2979
Size: 867 2112
Bme1580I G_kGCm'C
Cuts at: 0 776 2979
Size: 776 2203
BmrI ACTGGGnnnn_n'
Cuts at: 0 355 2979
Size: 355 2624
BmtI G_CTAG'C
Cuts at: 0 1219 2979
Size: 1219 1760
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 439 2979
Size: 439 2540
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 906 1614 2979
Size: 359 547 708 1365
Fragments arranged by size:
1365 708 547 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 2979
Size: 848 2131
BsaWI w'CCGG_w
Cuts at: 0 2414 2979
Size: 2414 565
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 975 1005 2979
Size: 975 30 1974
Fragments arranged by size:
1974 975 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 909 2979
Size: 909 2070
BseYI C'CCAG_C
Cuts at: 0 1169 1675 2568 2979
Size: 1169 506 893 411
Fragments arranged by size:
1169 893 506 411
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 773 983 1134 1925 2979
Size: 731 42 210 151 791 1054
Fragments arranged by size:
1054 791 731 210 151 42
BsiHKAI G_wGCw'C
Cuts at: 0 880 2943 2979
Size: 880 2063 36
Fragments arranged by size:
2063 880 36
BsmI GAATG_Cn'
Cuts at: 0 2201 2924 2969 2979
Size: 2201 723 45 10
Fragments arranged by size:
2201 723 45 10
Bsp1286I G_dGCh'C
Cuts at: 0 236 776 880 957 2139 2943 2979
Size: 236 540 104 77 1182 804 36
Fragments arranged by size:
1182 804 540 236 104 77 36
BspHI T'CATG_A
Cuts at: 0 2105 2915 2979
Size: 2105 810 64
Fragments arranged by size:
2105 810 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1220 2979
Size: 1220 1759
BsrDI GCAATG_nn'
Cuts at: 0 285 443 1655 2979
Size: 285 158 1212 1324
Fragments arranged by size:
1324 1212 285 158
BstBI TT'CG_AA
Cuts at: 0 2667 2979
Size: 2667 312
BstEII G'GTnAC_C
Cuts at: 0 472 2979
Size: 472 2507
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1177 1689 2387 2979
Size: 1177 512 698 592
Fragments arranged by size:
1177 698 592 512
BstYI r'GATC_y
Cuts at: 0 867 2979
Size: 867 2112
Bsu36I CC'TnA_GG
Cuts at: 0 316 2979
Size: 316 2663
BtgI C'CryG_G
Cuts at: 0 840 2558 2979
Size: 840 1718 421
Fragments arranged by size:
1718 840 421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1775 2979
Size: 1775 1204
BtsI GCAGTG_nn'
Cuts at: 0 714 744 756 1935 2979
Size: 714 30 12 1179 1044
Fragments arranged by size:
1179 1044 714 30 12
DraI TTT'AAA
Cuts at: 0 1785 2979
Size: 1785 1194
EaeI y'GGCC_r
Cuts at: 0 837 1932 2979
Size: 837 1095 1047
Fragments arranged by size:
1095 1047 837
EarI CTCTTCn'nnn_
Cuts at: 0 1760 2729 2979
Size: 1760 969 250
Fragments arranged by size:
1760 969 250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 856 906 1478 1614 2979
Size: 359 497 50 572 136 1365
Fragments arranged by size:
1365 572 497 359 136 50
EcoO109I rG'GnC_Cy
Cuts at: 0 1830 2256 2979
Size: 1830 426 723
Fragments arranged by size:
1830 723 426
EcoRI G'AATT_C
Cuts at: 0 1912 2648 2979
Size: 1912 736 331
Fragments arranged by size:
1912 736 331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 493 2979
Size: 493 2486
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 132 164 2979
Size: 132 32 2815
Fragments arranged by size:
2815 132 32
HincII GTy'rAC
Cuts at: 0 2283 2979
Size: 2283 696
HindIII A'AGCT_T
Cuts at: 0 496 2979
Size: 496 2483
KpnI G_GTAC'C
Cuts at: 0 21 2979
Size: 21 2958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 913 2573 2625 2748 2979
Size: 113 800 1660 52 123 231
Fragments arranged by size:
1660 800 231 123 113 52
MslI CAynn'nnrTG
Cuts at: 0 508 749 1204 1406 1660 1690 2060 2957
Size: 508 241 455 202 254 30 370 897
Cuts at: 2957 2974 2979
Size: 17 5
Fragments arranged by size:
897 508 455 370 254 241 202 30
17 5
MspA1I CmG'CkG
Cuts at: 0 1368 1624 2572 2824 2979
Size: 1368 256 948 252 155
Fragments arranged by size:
1368 948 256 252 155
NcoI C'CATG_G
Cuts at: 0 2558 2979
Size: 2558 421
NheI G'CTAG_C
Cuts at: 0 1215 2979
Size: 1215 1764
NsiI A_TGCA'T
Cuts at: 0 2148 2979
Size: 2148 831
NspI r_CATG'y
Cuts at: 0 701 983 1167 2505 2979
Size: 701 282 184 1338 474
Fragments arranged by size:
1338 701 474 282 184
PasI CC'CwG_GG
Cuts at: 0 2694 2979
Size: 2694 285
PciI A'CATG_T
Cuts at: 0 697 2501 2979
Size: 697 1804 478
Fragments arranged by size:
1804 697 478
PflMI CCAn_nnn'nTGG
Cuts at: 0 157 161 228 2979
Size: 157 4 67 2751
Fragments arranged by size:
2751 157 67 4
PfoI T'CCnGG_A
Cuts at: 0 336 2979
Size: 336 2643
PpuMI rG'GwC_Cy
Cuts at: 0 1830 2256 2979
Size: 1830 426 723
Fragments arranged by size:
1830 723 426
PshAI GACnn'nnGTC
Cuts at: 0 528 700 2979
Size: 528 172 2279
Fragments arranged by size:
2279 528 172
PsiI TTA'TAA
Cuts at: 0 2521 2979
Size: 2521 458
PstI C_TGCA'G
Cuts at: 0 453 751 1065 1367 1930 2979
Size: 453 298 314 302 563 1049
Fragments arranged by size:
1049 563 453 314 302 298
PvuII CAG'CTG
Cuts at: 0 1624 2572 2824 2979
Size: 1624 948 252 155
Fragments arranged by size:
1624 948 252 155
SapI GCTCTTCn'nnn_
Cuts at: 0 1760 2979
Size: 1760 1219
ScaI AGT'ACT
Cuts at: 0 98 344 1361 2979
Size: 98 246 1017 1618
Fragments arranged by size:
1618 1017 246 98
SexAI A'CCwGG_T
Cuts at: 0 212 2979
Size: 212 2767
SfcI C'TryA_G
Cuts at: 0 449 747 1061 1092 1363 1613 1619 1670
Size: 449 298 314 31 271 250 6 51
Cuts at: 1670 1926 2979
Size: 256 1053
Fragments arranged by size:
1053 449 314 298 271 256 250 51
31 6
SmlI C'TyrA_G
Cuts at: 0 863 1269 2979
Size: 863 406 1710
Fragments arranged by size:
1710 863 406
SphI G_CATG'C
Cuts at: 0 983 2979
Size: 983 1996
SspI AAT'ATT
Cuts at: 0 1100 2274 2979
Size: 1100 1174 705
Fragments arranged by size:
1174 1100 705
StuI AGG'CCT
Cuts at: 0 990 2979
Size: 990 1989
StyI C'CwwG_G
Cuts at: 0 468 477 1859 2558 2979
Size: 468 9 1382 699 421
Fragments arranged by size:
1382 699 468 421 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 149 168 2571 2979
Size: 149 19 2403 408
Fragments arranged by size:
2403 408 149 19
TatI w'GTAC_w
Cuts at: 0 96 342 374 599 1359 2979
Size: 96 246 32 225 760 1620
Fragments arranged by size:
1620 760 246 225 96 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 975 1007 2979
Size: 975 32 1972
Fragments arranged by size:
1972 975 32
XbaI T'CTAG_A
Cuts at: 0 2652 2979
Size: 2652 327
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 158 475 1041 2979
Size: 158 317 566 1938
Fragments arranged by size:
1938 566 317 158
XmnI GAAnn'nnTTC
Cuts at: 0 1136 2979
Size: 1136 1843
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AleI AlfI AlwNI
ApoI AseI AvaI BanI BanII BbsI BcgI BciVI
BfrBI BglI BglII Bme1580I BmrI BmtI BplI BpmI
BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI EaeI EarI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII HindIII KpnI MmeI
MslI MspA1I NcoI NheI NsiI NspI PasI PciI
PflMI PfoI PpuMI PshAI PsiI PstI PvuII SapI
ScaI SexAI SfcI SmlI SphI SspI StuI StyI
TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AloI ApaI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI
BclI BlpI BmgBI Bpu10I BsaI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstZ17I ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
HpaI KasI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NotI NruI PacI PmeI PmlI PpiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI Tth111I XhoI XmaI ZraI