Restriction map for
pF1KB4098
(Linear) (Six-base) MAPSORT of: KIBB4098.seq Check: 3968 from: 1 to: 1233
KIBB4098.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 233 1233
Size: 226 7 1000
Fragments arranged by size:
1000 226 7
AfeI AGC'GCT
Cuts at: 0 30 1233
Size: 30 1203
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 875 907 1233
Size: 875 32 326
Fragments arranged by size:
875 326 32
AlwNI CAG_nnn'CTG
Cuts at: 0 120 285 1233
Size: 120 165 948
Fragments arranged by size:
948 165 120
ApoI r'AATT_y
Cuts at: 0 301 697 1233
Size: 301 396 536
Fragments arranged by size:
536 396 301
AvaI C'yCGr_G
Cuts at: 0 62 1233
Size: 62 1171
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1170 1203 1233
Size: 1170 33 30
Fragments arranged by size:
1170 33 30
BanI G'GyrC_C
Cuts at: 0 46 57 1233
Size: 46 11 1176
Fragments arranged by size:
1176 46 11
BanII G_rGCy'C
Cuts at: 0 1168 1233
Size: 1168 65
BbeI G_GCGC'C
Cuts at: 0 50 1233
Size: 50 1183
BbsI GAAGACnn'nnnn_
Cuts at: 0 860 1094 1233
Size: 860 234 139
Fragments arranged by size:
860 234 139
BciVI GTATCCnnnnn_n'
Cuts at: 0 1198 1233
Size: 1198 35
BclI T'GATC_A
Cuts at: 0 645 1233
Size: 645 588
BfrBI ATG'CAT
Cuts at: 0 142 1233
Size: 142 1091
BglII A'GATC_T
Cuts at: 0 1038 1233
Size: 1038 195
Bme1580I G_kGCm'C
Cuts at: 0 62 1233
Size: 62 1171
BmrI ACTGGGnnnn_n'
Cuts at: 0 439 1233
Size: 439 794
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 96 1233
Size: 96 1137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1233
Size: 650 583
BsaBI GATnn'nnATC
Cuts at: 0 1043 1233
Size: 1043 190
BsaHI Gr'CG_yC
Cuts at: 0 47 1233
Size: 47 1186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 875 905 1233
Size: 875 30 328
Fragments arranged by size:
875 328 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 896 1233
Size: 896 337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 1233
Size: 923 310
BsiHKAI G_wGCw'C
Cuts at: 0 1168 1233
Size: 1168 65
BsmI GAATG_Cn'
Cuts at: 0 777 975 1233
Size: 777 198 258
Fragments arranged by size:
777 258 198
Bsp1286I G_dGCh'C
Cuts at: 0 62 1168 1233
Size: 62 1106 65
Fragments arranged by size:
1106 65 62
BsrDI GCAATG_nn'
Cuts at: 0 782 1233
Size: 782 451
BsrFI r'CCGG_y
Cuts at: 0 49 1233
Size: 49 1184
BsrGI T'GTAC_A
Cuts at: 0 433 1233
Size: 433 800
BstBI TT'CG_AA
Cuts at: 0 369 1233
Size: 369 864
BstYI r'GATC_y
Cuts at: 0 1038 1233
Size: 1038 195
BtsI GCAGTG_nn'
Cuts at: 0 611 935 1233
Size: 611 324 298
Fragments arranged by size:
611 324 298
ClaI AT'CG_AT
Cuts at: 0 480 1233
Size: 480 753
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1205 1233
Size: 1205 28
DraIII CAC_nnn'GTG
Cuts at: 0 723 1233
Size: 723 510
EaeI y'GGCC_r
Cuts at: 0 1226 1233
Size: 1226 7
EarI CTCTTCn'nnn_
Cuts at: 0 252 1233
Size: 252 981
EcoICRI GAG'CTC
Cuts at: 0 1166 1233
Size: 1166 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 233 650 1233
Size: 226 7 417 583
Fragments arranged by size:
583 417 226 7
EcoRI G'AATT_C
Cuts at: 0 301 1233
Size: 301 932
EcoRV GAT'ATC
Cuts at: 0 219 1233
Size: 219 1014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 173 874 1161 1233
Size: 173 701 287 72
Fragments arranged by size:
701 287 173 72
FspI TGC'GCA
Cuts at: 0 25 1233
Size: 25 1208
HaeII r_GCGC'y
Cuts at: 0 32 50 1233
Size: 32 18 1183
Fragments arranged by size:
1183 32 18
HincII GTy'rAC
Cuts at: 0 441 1233
Size: 441 792
HindIII A'AGCT_T
Cuts at: 0 676 1233
Size: 676 557
KasI G'GCGC_C
Cuts at: 0 46 1233
Size: 46 1187
MfeI C'AATT_G
Cuts at: 0 256 1233
Size: 256 977
MscI TGG'CCA
Cuts at: 0 1228 1233
Size: 1228 5
MslI CAynn'nnrTG
Cuts at: 0 383 1233
Size: 383 850
MspA1I CmG'CkG
Cuts at: 0 102 1233
Size: 102 1131
NaeI GCC'GGC
Cuts at: 0 51 1233
Size: 51 1182
NarI GG'CG_CC
Cuts at: 0 47 1233
Size: 47 1186
NdeI CA'TA_TG
Cuts at: 0 749 1233
Size: 749 484
NgoMIV G'CCGG_C
Cuts at: 0 49 1233
Size: 49 1184
NsiI A_TGCA'T
Cuts at: 0 144 1233
Size: 144 1089
NspI r_CATG'y
Cuts at: 0 146 1233
Size: 146 1087
PfoI T'CCnGG_A
Cuts at: 0 964 1233
Size: 964 269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 875 907 1233
Size: 875 32 326
Fragments arranged by size:
875 326 32
SacI G_AGCT'C
Cuts at: 0 1168 1233
Size: 1168 65
ScaI AGT'ACT
Cuts at: 0 1027 1233
Size: 1027 206
SfcI C'TryA_G
Cuts at: 0 87 1112 1233
Size: 87 1025 121
Fragments arranged by size:
1025 121 87
SfoI GGC'GCC
Cuts at: 0 48 1233
Size: 48 1185
SspI AAT'ATT
Cuts at: 0 469 1233
Size: 469 764
TatI w'GTAC_w
Cuts at: 0 433 1025 1233
Size: 433 592 208
Fragments arranged by size:
592 433 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 627 659 1233
Size: 627 32 574
Fragments arranged by size:
627 574 32
Tth111I GACn'n_nGTC
Cuts at: 0 445 1233
Size: 445 788
XmnI GAAnn'nnTTC
Cuts at: 0 1121 1233
Size: 1121 112
Enzymes that do cut:
AcuI AfeI AloI AlwNI ApoI AvaI BaeI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII Bme1580I
BmrI BplI BpmI BsaBI BsaHI BsaXI BseRI BsgI
BsiHKAI BsmI Bsp1286I BsrDI BsrFI BsrGI BstBI BstYI
BtsI ClaI CspCI DraIII EaeI EarI EcoICRI Eco57MI
EcoRI EcoRV FalI FspI HaeII HincII HindIII KasI
MfeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PfoI PpiI SacI ScaI SfcI SfoI
SspI TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI
AvrII BamHI BbvCI BcgI BglI BlpI BmgBI BmtI
Bpu10I BpuEI BsaI BsaAI BsaWI BseYI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BssHII BssSI BstAPI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI DraI DrdI
EagI EciI EcoNI EcoO109I FseI FspAI Hin4I HpaI
KpnI MluI MmeI NcoI NheI NotI NruI PacI
PasI PciI PflMI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
SwaI TaqII XbaI XcmI XhoI XmaI ZraI