Restriction map for
pF1KB4086
(Linear) (Six-base) MAPSORT of: KIBB4086.seq Check: 6359 from: 1 to: 1560
KIBB4086.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 139 704 1560
Size: 139 565 856
Fragments arranged by size:
856 565 139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 963 1025 1550 1560
Size: 963 62 525 10
Fragments arranged by size:
963 525 62 10
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 48 1560
Size: 48 1512
AlwNI CAG_nnn'CTG
Cuts at: 0 403 1528 1560
Size: 403 1125 32
Fragments arranged by size:
1125 403 32
BanI G'GyrC_C
Cuts at: 0 1254 1560
Size: 1254 306
BbsI GAAGACnn'nnnn_
Cuts at: 0 319 399 926 1257 1560
Size: 319 80 527 331 303
Fragments arranged by size:
527 331 319 303 80
BbvCI CC'TCA_GC
Cuts at: 0 1085 1560
Size: 1085 475
BclI T'GATC_A
Cuts at: 0 93 1560
Size: 93 1467
BfrBI ATG'CAT
Cuts at: 0 382 1560
Size: 382 1178
BglII A'GATC_T
Cuts at: 0 68 1560
Size: 68 1492
BmrI ACTGGGnnnn_n'
Cuts at: 0 720 1560
Size: 720 840
BmtI G_CTAG'C
Cuts at: 0 968 1560
Size: 968 592
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 453 1560
Size: 453 1107
Bpu10I CC'TnA_GC
Cuts at: 0 1085 1560
Size: 1085 475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 1560
Size: 85 1475
BsaHI Gr'CG_yC
Cuts at: 0 1404 1560
Size: 1404 156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1490 1520 1560
Size: 1490 30 40
Fragments arranged by size:
1490 40 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 1560
Size: 159 1401
BsmI GAATG_Cn'
Cuts at: 0 27 1452 1560
Size: 27 1425 108
Fragments arranged by size:
1425 108 27
BspHI T'CATG_A
Cuts at: 0 779 1560
Size: 779 781
BsrGI T'GTAC_A
Cuts at: 0 1031 1560
Size: 1031 529
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 1560
Size: 150 1410
BstYI r'GATC_y
Cuts at: 0 68 716 1560
Size: 68 648 844
Fragments arranged by size:
844 648 68
BstZ17I GTA'TAC
Cuts at: 0 140 1560
Size: 140 1420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 29 64 1560
Size: 29 35 1496
Fragments arranged by size:
1496 35 29
EaeI y'GGCC_r
Cuts at: 0 37 1560
Size: 37 1523
EarI CTCTTCn'nnn_
Cuts at: 0 332 982 1096 1560
Size: 332 650 114 464
Fragments arranged by size:
650 464 332 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 963 1025 1550 1560
Size: 963 62 525 10
Fragments arranged by size:
963 525 62 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 186 1560
Size: 186 1374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 613 645 1210 1242 1560
Size: 181 32 400 32 565 32 318
Fragments arranged by size:
565 400 318 181 32 32 32
HincII GTy'rAC
Cuts at: 0 420 1311 1560
Size: 420 891 249
Fragments arranged by size:
891 420 249
HindIII A'AGCT_T
Cuts at: 0 29 1560
Size: 29 1531
HpaI GTT'AAC
Cuts at: 0 420 1560
Size: 420 1140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 255 501 1560
Size: 86 169 246 1059
Fragments arranged by size:
1059 246 169 86
MscI TGG'CCA
Cuts at: 0 39 1560
Size: 39 1521
MslI CAynn'nnrTG
Cuts at: 0 211 602 1560
Size: 211 391 958
Fragments arranged by size:
958 391 211
MspA1I CmG'CkG
Cuts at: 0 330 400 692 1537 1560
Size: 330 70 292 845 23
Fragments arranged by size:
845 330 292 70 23
NdeI CA'TA_TG
Cuts at: 0 590 1560
Size: 590 970
NheI G'CTAG_C
Cuts at: 0 964 1560
Size: 964 596
NsiI A_TGCA'T
Cuts at: 0 384 1560
Size: 384 1176
NspI r_CATG'y
Cuts at: 0 386 601 1560
Size: 386 215 959
Fragments arranged by size:
959 386 215
PasI CC'CwG_GG
Cuts at: 0 1323 1560
Size: 1323 237
PflMI CCAn_nnn'nTGG
Cuts at: 0 440 1305 1560
Size: 440 865 255
Fragments arranged by size:
865 440 255
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1383 1415 1560
Size: 1383 32 145
Fragments arranged by size:
1383 145 32
PvuII CAG'CTG
Cuts at: 0 330 400 1537 1560
Size: 330 70 1137 23
Fragments arranged by size:
1137 330 70 23
ScaI AGT'ACT
Cuts at: 0 1078 1560
Size: 1078 482
SfcI C'TryA_G
Cuts at: 0 509 641 882 1560
Size: 509 132 241 678
Fragments arranged by size:
678 509 241 132
SmlI C'TyrA_G
Cuts at: 0 64 1560
Size: 64 1496
SphI G_CATG'C
Cuts at: 0 386 1560
Size: 386 1174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1312 1560
Size: 1312 248
TatI w'GTAC_w
Cuts at: 0 1031 1076 1148 1560
Size: 1031 45 72 412
Fragments arranged by size:
1031 412 72 45
XmnI GAAnn'nnTTC
Cuts at: 0 844 1560
Size: 844 716
Enzymes that do cut:
AccI AcuI AlfI AlwNI BanI BbsI BbvCI BclI
BfrBI BglII BmrI BmtI BplI Bpu10I BpuEI BsaHI
BsaXI BsgI BsmI BspHI BsrGI BstXI BstYI BstZ17I
CspCI EaeI EarI Eco57MI FalI Hin4I HincII HindIII
HpaI MmeI MscI MslI MspA1I NdeI NheI NsiI
NspI PasI PflMI PpiI PvuII ScaI SfcI SmlI
SphI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BcgI
BciVI BglI BlpI Bme1580I BmgBI BpmI BsaI BsaAI
BsaBI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII KasI KpnI
MfeI MluI NaeI NarI NcoI NgoMIV NotI NruI
PacI PciI PfoI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI