Restriction map for
pF1KB4085
(Linear) (Six-base) MAPSORT of: KIBB4085.seq Check: 3155 from: 1 to: 2919
KIBB4085.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1740 2919
Size: 1740 1179
AatII G_ACGT'C
Cuts at: 0 2077 2131 2919
Size: 2077 54 788
Fragments arranged by size:
2077 788 54
AccI GT'mk_AC
Cuts at: 0 200 2919
Size: 200 2719
Acc65I G'GTAC_C
Cuts at: 0 513 1192 1836 2919
Size: 513 679 644 1083
Fragments arranged by size:
1083 679 644 513
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1160 2394 2407 2919
Size: 1160 1234 13 512
Fragments arranged by size:
1234 1160 512 13
AflIII A'CryG_T
Cuts at: 0 1616 2186 2919
Size: 1616 570 733
Fragments arranged by size:
1616 733 570
AleI CACnn'nnGTG
Cuts at: 0 226 874 2311 2919
Size: 226 648 1437 608
Fragments arranged by size:
1437 648 608 226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1196 2919
Size: 1196 1723
AlwNI CAG_nnn'CTG
Cuts at: 0 135 249 769 2919
Size: 135 114 520 2150
Fragments arranged by size:
2150 520 135 114
ApaLI G'TGCA_C
Cuts at: 0 1624 2527 2919
Size: 1624 903 392
Fragments arranged by size:
1624 903 392
ApoI r'AATT_y
Cuts at: 0 354 624 923 1004 1046 2728 2919
Size: 354 270 299 81 42 1682 191
Fragments arranged by size:
1682 354 299 270 191 81 42
AvaI C'yCGr_G
Cuts at: 0 120 1468 2432 2919
Size: 120 1348 964 487
Fragments arranged by size:
1348 964 487 120
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1828 1861 2919
Size: 1828 33 1058
Fragments arranged by size:
1828 1058 33
BamHI G'GATC_C
Cuts at: 0 124 2157 2919
Size: 124 2033 762
Fragments arranged by size:
2033 762 124
BanI G'GyrC_C
Cuts at: 0 220 273 513 1192 1836 2919
Size: 220 53 240 679 644 1083
Fragments arranged by size:
1083 679 644 240 220 53
BanII G_rGCy'C
Cuts at: 0 1390 2066 2386 2919
Size: 1390 676 320 533
Fragments arranged by size:
1390 676 533 320
BciVI GTATCCnnnnn_n'
Cuts at: 0 415 2604 2919
Size: 415 2189 315
Fragments arranged by size:
2189 415 315
BclI T'GATC_A
Cuts at: 0 2574 2919
Size: 2574 345
BlpI GC'TnA_GC
Cuts at: 0 2265 2919
Size: 2265 654
Bme1580I G_kGCm'C
Cuts at: 0 1628 2531 2919
Size: 1628 903 388
Fragments arranged by size:
1628 903 388
BmgBI CAC'GTC
Cuts at: 0 456 1122 1437 2919
Size: 456 666 315 1482
Fragments arranged by size:
1482 666 456 315
BmrI ACTGGGnnnn_n'
Cuts at: 0 2450 2919
Size: 2450 469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 340 433 1253 1699 2304 2621 2919
Size: 277 63 93 820 446 605 317 298
Fragments arranged by size:
820 605 446 317 298 277 93 63
Bpu10I CC'TnA_GC
Cuts at: 0 242 1184 2919
Size: 242 942 1735
Fragments arranged by size:
1735 942 242
BsaI GGTCTCn'nnnn_
Cuts at: 0 2824 2919
Size: 2824 95
BsaAI yAC'GTr
Cuts at: 0 1617 1863 1965 2919
Size: 1617 246 102 954
Fragments arranged by size:
1617 954 246 102
BsaBI GATnn'nnATC
Cuts at: 0 736 2919
Size: 736 2183
BsaHI Gr'CG_yC
Cuts at: 0 295 1712 2074 2128 2919
Size: 295 1417 362 54 791
Fragments arranged by size:
1417 791 362 295 54
BsaWI w'CCGG_w
Cuts at: 0 2704 2919
Size: 2704 215
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1148 1178 1808 1838 1879 1909 2482 2512
Size: 1148 30 630 30 41 30 573 30
Cuts at: 2512 2919
Size: 407
Fragments arranged by size:
1148 630 573 407 41 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 1687 2370 2551 2842 2919
Size: 220 1467 683 181 291 77
Fragments arranged by size:
1467 683 291 220 181 77
BseYI C'CCAG_C
Cuts at: 0 128 829 2919
Size: 128 701 2090
Fragments arranged by size:
2090 701 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 2919
Size: 420 2499
BsiEI CG_ry'CG
Cuts at: 0 369 1846 1943 2919
Size: 369 1477 97 976
Fragments arranged by size:
1477 976 369 97
BsiHKAI G_wGCw'C
Cuts at: 0 1601 1628 2234 2320 2374 2531 2919
Size: 1601 27 606 86 54 157 388
Fragments arranged by size:
1601 606 388 157 86 54 27
BsmI GAATG_Cn'
Cuts at: 0 2546 2919
Size: 2546 373
Bsp1286I G_dGCh'C
Cuts at: 0 1390 1601 1628 2066 2234 2320 2374 2386
Size: 1390 211 27 438 168 86 54 12
Cuts at: 2386 2531 2919
Size: 145 388
Fragments arranged by size:
1390 438 388 211 168 145 86 54
27 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 429 1078 1740 2919
Size: 429 649 662 1179
Fragments arranged by size:
1179 662 649 429
BsrBI CCG'CTC
Cuts at: 0 179 1584 1637 2919
Size: 179 1405 53 1282
Fragments arranged by size:
1405 1282 179 53
BsrDI GCAATG_nn'
Cuts at: 0 101 1199 2919
Size: 101 1098 1720
Fragments arranged by size:
1720 1098 101
BsrFI r'CCGG_y
Cuts at: 0 486 1714 1782 1932 2285 2919
Size: 486 1228 68 150 353 634
Fragments arranged by size:
1228 634 486 353 150 68
BsrGI T'GTAC_A
Cuts at: 0 1973 2246 2919
Size: 1973 273 673
Fragments arranged by size:
1973 673 273
BssHII G'CGCG_C
Cuts at: 0 1703 1705 1878 1946 2291 2919
Size: 1703 2 173 68 345 628
Fragments arranged by size:
1703 628 345 173 68 2
BssSI C'ACGA_G
Cuts at: 0 1669 2919
Size: 1669 1250
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2091 2226 2328 2919
Size: 2091 135 102 591
Fragments arranged by size:
2091 591 135 102
BstYI r'GATC_y
Cuts at: 0 124 2157 2743 2919
Size: 124 2033 586 176
Fragments arranged by size:
2033 586 176 124
Bsu36I CC'TnA_GG
Cuts at: 0 38 2919
Size: 38 2881
BtgI C'CryG_G
Cuts at: 0 1082 1406 1421 1840 2055 2078 2084 2327
Size: 1082 324 15 419 215 23 6 243
Cuts at: 2327 2919
Size: 592
Fragments arranged by size:
1082 592 419 324 243 215 23 15
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 222 2184 2919
Size: 222 1962 735
Fragments arranged by size:
1962 735 222
BtsI GCAGTG_nn'
Cuts at: 0 1786 2249 2587 2919
Size: 1786 463 338 332
Fragments arranged by size:
1786 463 338 332
ClaI AT'CG_AT
Cuts at: 0 740 2919
Size: 740 2179
DraI TTT'AAA
Cuts at: 0 2447 2676 2919
Size: 2447 229 243
Fragments arranged by size:
2447 243 229
DraIII CAC_nnn'GTG
Cuts at: 0 1866 2919
Size: 1866 1053
EaeI y'GGCC_r
Cuts at: 0 180 1430 2081 2112 2584 2919
Size: 180 1250 651 31 472 335
Fragments arranged by size:
1250 651 472 335 180 31
EarI CTCTTCn'nnn_
Cuts at: 0 1022 2919
Size: 1022 1897
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 280 402 2029 2919
Size: 280 122 1627 890
Fragments arranged by size:
1627 890 280 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 340 433 1160 1253 1699 2304 2394
Size: 277 63 93 727 93 446 605 90
Cuts at: 2394 2407 2621 2919
Size: 13 214 298
Fragments arranged by size:
727 605 446 298 277 214 93 93
90 63 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 2008 2536 2919
Size: 319 1689 528 383
Fragments arranged by size:
1689 528 383 319
EcoO109I rG'GnC_Cy
Cuts at: 0 80 246 312 2118 2569 2919
Size: 80 166 66 1806 451 350
Fragments arranged by size:
1806 451 350 166 80 66
EcoRI G'AATT_C
Cuts at: 0 354 2919
Size: 354 2565
EcoRV GAT'ATC
Cuts at: 0 579 2919
Size: 579 2340
HaeII r_GCGC'y
Cuts at: 0 566 1331 2153 2272 2327 2919
Size: 566 765 822 119 55 592
Fragments arranged by size:
822 765 592 566 119 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1213 1235 1245 1267 1333 1365 1372 1404
Size: 1213 22 10 22 66 32 7 32
Cuts at: 1404 1879 1911 2919
Size: 475 32 1008
Fragments arranged by size:
1213 1008 475 66 32 32 32 22
22 10 7
HincII GTy'rAC
Cuts at: 0 1056 2655 2919
Size: 1056 1599 264
Fragments arranged by size:
1599 1056 264
HpaI GTT'AAC
Cuts at: 0 1056 2919
Size: 1056 1863
KpnI G_GTAC'C
Cuts at: 0 517 1196 1840 2919
Size: 517 679 644 1079
Fragments arranged by size:
1079 679 644 517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 1199 2919
Size: 906 293 1720
Fragments arranged by size:
1720 906 293
MscI TGG'CCA
Cuts at: 0 2586 2919
Size: 2586 333
MslI CAynn'nnrTG
Cuts at: 0 226 874 2311 2919
Size: 226 648 1437 608
Fragments arranged by size:
1437 648 608 226
MspA1I CmG'CkG
Cuts at: 0 435 1842 2427 2666 2919
Size: 435 1407 585 239 253
Fragments arranged by size:
1407 585 435 253 239
NaeI GCC'GGC
Cuts at: 0 1716 1784 2919
Size: 1716 68 1135
Fragments arranged by size:
1716 1135 68
NcoI C'CATG_G
Cuts at: 0 1082 2919
Size: 1082 1837
NgoMIV G'CCGG_C
Cuts at: 0 1714 1782 2919
Size: 1714 68 1137
Fragments arranged by size:
1714 1137 68
NspI r_CATG'y
Cuts at: 0 2517 2919
Size: 2517 402
PflMI CCAn_nnn'nTGG
Cuts at: 0 1866 2919
Size: 1866 1053
PfoI T'CCnGG_A
Cuts at: 0 791 2003 2562 2919
Size: 791 1212 559 357
Fragments arranged by size:
1212 791 559 357
PmlI CAC'GTG
Cuts at: 0 1863 1965 2919
Size: 1863 102 954
Fragments arranged by size:
1863 954 102
PpuMI rG'GwC_Cy
Cuts at: 0 246 312 2919
Size: 246 66 2607
Fragments arranged by size:
2607 246 66
PshAI GACnn'nnGTC
Cuts at: 0 809 2919
Size: 809 2110
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 497 529 1465 1497 2919
Size: 497 32 936 32 1422
Fragments arranged by size:
1422 936 497 32 32
PstI C_TGCA'G
Cuts at: 0 335 440 1658 1691 1751 2379 2919
Size: 335 105 1218 33 60 628 540
Fragments arranged by size:
1218 628 540 335 105 60 33
PvuII CAG'CTG
Cuts at: 0 2427 2666 2919
Size: 2427 239 253
Fragments arranged by size:
2427 253 239
SacII CC_GC'GG
Cuts at: 0 1843 2919
Size: 1843 1076
SfcI C'TryA_G
Cuts at: 0 331 436 1654 1687 1747 2375 2919
Size: 331 105 1218 33 60 628 544
Fragments arranged by size:
1218 628 544 331 105 60 33
SgrAI Cr'CCGG_yG
Cuts at: 0 486 1714 2919
Size: 486 1228 1205
Fragments arranged by size:
1228 1205 486
SmaI CCC'GGG
Cuts at: 0 122 2919
Size: 122 2797
SmlI C'TyrA_G
Cuts at: 0 2432 2919
Size: 2432 487
SphI G_CATG'C
Cuts at: 0 2517 2919
Size: 2517 402
StyI C'CwwG_G
Cuts at: 0 1082 2919
Size: 1082 1837
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1840 2919
Size: 1840 1079
TatI w'GTAC_w
Cuts at: 0 2 1472 1973 2246 2919
Size: 2 1470 501 273 673
Fragments arranged by size:
1470 673 501 273 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1148 1180 2919
Size: 1148 32 1739
Fragments arranged by size:
1739 1148 32
Tth111I GACn'n_nGTC
Cuts at: 0 1132 2130 2919
Size: 1132 998 789
Fragments arranged by size:
1132 998 789
XbaI T'CTAG_A
Cuts at: 0 555 2919
Size: 555 2364
XhoI C'TCGA_G
Cuts at: 0 2432 2919
Size: 2432 487
XmaI C'CCGG_G
Cuts at: 0 120 2919
Size: 120 2799
XmnI GAAnn'nnTTC
Cuts at: 0 677 2919
Size: 677 2242
ZraI GAC'GTC
Cuts at: 0 2075 2129 2919
Size: 2075 54 790
Fragments arranged by size:
2075 790 54
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BciVI BclI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII
HpaI KpnI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PflMI PfoI PmlI PpuMI PshAI PsrI
PstI PvuII SacII SfcI SgrAI SmaI SmlI SphI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AloI ApaI AscI
AseI AsiSI AvrII BbeI BbsI BbvCI BcgI BfrBI
BglI BglII BmtI BplI BpuEI BsiWI BsmBI BspEI
BspHI BstAPI BstBI BstEII BstZ17I CspCI DrdI EagI
EcoICRI FalI FseI FspI FspAI HindIII KasI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PmeI PpiI PsiI PspOMI PspXI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SnaBI SpeI SrfI SspI StuI
SwaI XcmI