Restriction map for
pF1KB4076
(Linear) (Six-base) MAPSORT of: KIBB4076.seq Check: 4885 from: 1 to: 1719
KIBB4076.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 972 1560 1719
Size: 804 168 588 159
Fragments arranged by size:
804 588 168 159
AfeI AGC'GCT
Cuts at: 0 1658 1719
Size: 1658 61
AflIII A'CryG_T
Cuts at: 0 1048 1719
Size: 1048 671
AleI CACnn'nnGTG
Cuts at: 0 698 1719
Size: 698 1021
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 1719
Size: 136 32 1551
Fragments arranged by size:
1551 136 32
AlwNI CAG_nnn'CTG
Cuts at: 0 265 1719
Size: 265 1454
ApoI r'AATT_y
Cuts at: 0 233 814 1274 1526 1719
Size: 233 581 460 252 193
Fragments arranged by size:
581 460 252 233 193
BanII G_rGCy'C
Cuts at: 0 215 1719
Size: 215 1504
BbsI GAAGACnn'nnnn_
Cuts at: 0 511 1719
Size: 511 1208
BciVI GTATCCnnnnn_n'
Cuts at: 0 1436 1678 1719
Size: 1436 242 41
Fragments arranged by size:
1436 242 41
BglII A'GATC_T
Cuts at: 0 1249 1719
Size: 1249 470
BlpI GC'TnA_GC
Cuts at: 0 101 530 1719
Size: 101 429 1189
Fragments arranged by size:
1189 429 101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 504 1719
Size: 504 1215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 884 1136 1388 1472 1640 1719
Size: 151 733 252 252 84 168 79
Fragments arranged by size:
733 252 252 168 151 84 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 238 392 1537 1719
Size: 238 154 1145 182
Fragments arranged by size:
1145 238 182 154
BsaWI w'CCGG_w
Cuts at: 0 191 1496 1719
Size: 191 1305 223
Fragments arranged by size:
1305 223 191
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1719
Size: 91 1628
BsiHKAI G_wGCw'C
Cuts at: 0 1168 1719
Size: 1168 551
BsmI GAATG_Cn'
Cuts at: 0 451 1222 1474 1719
Size: 451 771 252 245
Fragments arranged by size:
771 451 252 245
Bsp1286I G_dGCh'C
Cuts at: 0 215 1168 1719
Size: 215 953 551
Fragments arranged by size:
953 551 215
BspEI T'CCGG_A
Cuts at: 0 1496 1719
Size: 1496 223
BspHI T'CATG_A
Cuts at: 0 1389 1719
Size: 1389 330
BsrDI GCAATG_nn'
Cuts at: 0 1227 1719
Size: 1227 492
BsrGI T'GTAC_A
Cuts at: 0 113 1043 1719
Size: 113 930 676
Fragments arranged by size:
930 676 113
BstEII G'GTnAC_C
Cuts at: 0 39 1719
Size: 39 1680
BstYI r'GATC_y
Cuts at: 0 1249 1719
Size: 1249 470
BtsI GCAGTG_nn'
Cuts at: 0 95 1719
Size: 95 1624
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1209 1244 1629 1664 1719
Size: 1209 35 385 35 55
Fragments arranged by size:
1209 385 55 35 35
DraI TTT'AAA
Cuts at: 0 309 584 1719
Size: 309 275 1135
Fragments arranged by size:
1135 309 275
EarI CTCTTCn'nnn_
Cuts at: 0 203 1535 1719
Size: 203 1332 184
Fragments arranged by size:
1332 203 184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 804 884 972 1136 1388 1472 1560
Size: 151 653 80 88 164 252 84 88
Cuts at: 1560 1640 1719
Size: 80 79
Fragments arranged by size:
653 252 164 151 88 88 84 80
80 79
EcoO109I rG'GnC_Cy
Cuts at: 0 1208 1719
Size: 1208 511
EcoRI G'AATT_C
Cuts at: 0 1274 1526 1719
Size: 1274 252 193
Fragments arranged by size:
1274 252 193
HaeII r_GCGC'y
Cuts at: 0 831 1660 1719
Size: 831 829 59
Fragments arranged by size:
831 829 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 304 336 1155 1187 1719
Size: 27 277 32 819 32 532
Fragments arranged by size:
819 532 277 32 32 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1515 1719
Size: 176 1339 204
Fragments arranged by size:
1339 204 176
MslI CAynn'nnrTG
Cuts at: 0 334 560 698 947 1383 1394 1467 1719
Size: 334 226 138 249 436 11 73 252
Fragments arranged by size:
436 334 252 249 226 138 73 11
MspA1I CmG'CkG
Cuts at: 0 824 992 1719
Size: 824 168 727
Fragments arranged by size:
824 727 168
NdeI CA'TA_TG
Cuts at: 0 1380 1464 1719
Size: 1380 84 255
Fragments arranged by size:
1380 255 84
NspI r_CATG'y
Cuts at: 0 1052 1640 1719
Size: 1052 588 79
Fragments arranged by size:
1052 588 79
PciI A'CATG_T
Cuts at: 0 1048 1719
Size: 1048 671
PfoI T'CCnGG_A
Cuts at: 0 28 1719
Size: 28 1691
PvuII CAG'CTG
Cuts at: 0 824 992 1719
Size: 824 168 727
Fragments arranged by size:
824 727 168
SapI GCTCTTCn'nnn_
Cuts at: 0 203 1719
Size: 203 1516
ScaI AGT'ACT
Cuts at: 0 470 539 1719
Size: 470 69 1180
Fragments arranged by size:
1180 470 69
SexAI A'CCwGG_T
Cuts at: 0 146 1719
Size: 146 1573
StyI C'CwwG_G
Cuts at: 0 155 1719
Size: 155 1564
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 472 1719
Size: 472 1247
TatI w'GTAC_w
Cuts at: 0 113 468 537 1043 1719
Size: 113 355 69 506 676
Fragments arranged by size:
676 506 355 113 69
XmnI GAAnn'nnTTC
Cuts at: 0 1153 1489 1719
Size: 1153 336 230
Fragments arranged by size:
1153 336 230
Enzymes that do cut:
AcuI AfeI AflIII AleI AloI AlwNI ApoI BanII
BbsI BciVI BglII BlpI BplI BpmI BsaI BsaWI
BseRI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrGI
BstEII BstYI BtsI CspCI DraI EarI Eco57MI EcoO109I
EcoRI HaeII Hin4I MmeI MslI MspA1I NdeI NspI
PciI PfoI PvuII SapI ScaI SexAI StyI TaqII
TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BclI BfrBI
BglI Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiWI BsmBI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI