Restriction map for
pF1KB4055
(Linear) (Six-base) MAPSORT of: KIBB4055.seq Check: 3868 from: 1 to: 999
KIBB4055.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 0 546 999
Size: 546 453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 769 999
Size: 680 89 230
Fragments arranged by size:
680 230 89
AhdI GACnn_n'nnGTC
Cuts at: 0 587 999
Size: 587 412
AleI CACnn'nnGTG
Cuts at: 0 733 999
Size: 733 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 279 999
Size: 279 720
ApoI r'AATT_y
Cuts at: 0 105 121 325 346 373 999
Size: 105 16 204 21 27 626
Fragments arranged by size:
626 204 105 27 21 16
BanI G'GyrC_C
Cuts at: 0 935 999
Size: 935 64
BanII G_rGCy'C
Cuts at: 0 857 976 999
Size: 857 119 23
Fragments arranged by size:
857 119 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 888 999
Size: 888 111
BfrBI ATG'CAT
Cuts at: 0 704 999
Size: 704 295
BlpI GC'TnA_GC
Cuts at: 0 805 999
Size: 805 194
Bme1580I G_kGCm'C
Cuts at: 0 938 999
Size: 938 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 851 999
Size: 851 148
Bpu10I CC'TnA_GC
Cuts at: 0 877 999
Size: 877 122
BsaHI Gr'CG_yC
Cuts at: 0 543 999
Size: 543 456
BsaWI w'CCGG_w
Cuts at: 0 755 999
Size: 755 244
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 774 999
Size: 283 491 225
Fragments arranged by size:
491 283 225
BseYI C'CCAG_C
Cuts at: 0 35 999
Size: 35 964
Bsp1286I G_dGCh'C
Cuts at: 0 857 938 976 999
Size: 857 81 38 23
Fragments arranged by size:
857 81 38 23
BspHI T'CATG_A
Cuts at: 0 482 999
Size: 482 517
BsrGI T'GTAC_A
Cuts at: 0 944 999
Size: 944 55
BstAPI GCAn_nnn'nTGC
Cuts at: 0 508 691 999
Size: 508 183 308
Fragments arranged by size:
508 308 183
BstYI r'GATC_y
Cuts at: 0 476 531 999
Size: 476 55 468
Fragments arranged by size:
476 468 55
ClaI AT'CG_AT
Cuts at: 0 79 999
Size: 79 920
DraIII CAC_nnn'GTG
Cuts at: 0 942 999
Size: 942 57
DrdI GACnn_nn'nnGTC
Cuts at: 0 798 999
Size: 798 201
EaeI y'GGCC_r
Cuts at: 0 210 815 999
Size: 210 605 184
Fragments arranged by size:
605 210 184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 769 999
Size: 680 89 230
Fragments arranged by size:
680 230 89
EcoNI CCTnn'n_nnAGG
Cuts at: 0 134 999
Size: 134 865
EcoO109I rG'GnC_Cy
Cuts at: 0 429 999
Size: 429 570
EcoRI G'AATT_C
Cuts at: 0 105 346 999
Size: 105 241 653
Fragments arranged by size:
653 241 105
EcoRV GAT'ATC
Cuts at: 0 321 999
Size: 321 678
HaeII r_GCGC'y
Cuts at: 0 638 999
Size: 638 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 573 605 692 724 999
Size: 573 32 87 32 275
Fragments arranged by size:
573 275 87 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 559 999
Size: 471 88 440
Fragments arranged by size:
471 440 88
MscI TGG'CCA
Cuts at: 0 212 817 999
Size: 212 605 182
Fragments arranged by size:
605 212 182
MslI CAynn'nnrTG
Cuts at: 0 733 999
Size: 733 266
MspA1I CmG'CkG
Cuts at: 0 833 923 999
Size: 833 90 76
Fragments arranged by size:
833 90 76
NsiI A_TGCA'T
Cuts at: 0 706 999
Size: 706 293
PfoI T'CCnGG_A
Cuts at: 0 953 999
Size: 953 46
PsiI TTA'TAA
Cuts at: 0 227 999
Size: 227 772
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 973 999
Size: 973 26
ScaI AGT'ACT
Cuts at: 0 31 999
Size: 31 968
SspI AAT'ATT
Cuts at: 0 223 999
Size: 223 776
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 837 999
Size: 837 162
TatI w'GTAC_w
Cuts at: 0 29 944 999
Size: 29 915 55
Fragments arranged by size:
915 55 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 999
Size: 7 32 960
Fragments arranged by size:
960 32 7
Tth111I GACn'n_nGTC
Cuts at: 0 892 999
Size: 892 107
XbaI T'CTAG_A
Cuts at: 0 369 999
Size: 369 630
XmnI GAAnn'nnTTC
Cuts at: 0 576 999
Size: 576 423
ZraI GAC'GTC
Cuts at: 0 544 999
Size: 544 455
Enzymes that do cut:
AatII AcuI AhdI AleI AlfI ApoI BanI BanII
BbsI BfrBI BlpI Bme1580I BmrI Bpu10I BsaHI BsaWI
BseRI BseYI Bsp1286I BspHI BsrGI BstAPI BstYI ClaI
DraIII DrdI EaeI Eco57MI EcoNI EcoO109I EcoRI EcoRV
HaeII Hin4I MmeI MscI MslI MspA1I NsiI PfoI
PsiI PsrI ScaI SspI TaqII TatI TstI Tth111I
XbaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BglI BglII BmgBI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraI EagI EarI EciI EcoICRI FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI SwaI XcmI XhoI
XmaI