Restriction map for
pF1KB4035
(Linear) (Six-base) MAPSORT of: KIBB4035.seq Check: 2288 from: 1 to: 1419
KIBB4035.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 0 821 1419
Size: 821 598
Acc65I G'GTAC_C
Cuts at: 0 958 1419
Size: 958 461
AfeI AGC'GCT
Cuts at: 0 1020 1419
Size: 1020 399
AhdI GACnn_n'nnGTC
Cuts at: 0 612 1419
Size: 612 807
AlwNI CAG_nnn'CTG
Cuts at: 0 811 1042 1180 1419
Size: 811 231 138 239
Fragments arranged by size:
811 239 231 138
ApoI r'AATT_y
Cuts at: 0 73 732 1324 1419
Size: 73 659 592 95
Fragments arranged by size:
659 592 95 73
BanI G'GyrC_C
Cuts at: 0 625 958 1419
Size: 625 333 461
Fragments arranged by size:
625 461 333
BclI T'GATC_A
Cuts at: 0 134 380 1419
Size: 134 246 1039
Fragments arranged by size:
1039 246 134
BglI GCCn_nnn'nGGC
Cuts at: 0 281 1419
Size: 281 1138
BglII A'GATC_T
Cuts at: 0 759 1252 1419
Size: 759 493 167
Fragments arranged by size:
759 493 167
BlpI GC'TnA_GC
Cuts at: 0 103 1419
Size: 103 1316
BmgBI CAC'GTC
Cuts at: 0 614 1419
Size: 614 805
BmrI ACTGGGnnnn_n'
Cuts at: 0 436 499 849 874 1419
Size: 436 63 350 25 545
Fragments arranged by size:
545 436 350 63 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1290 1419
Size: 184 1106 129
Fragments arranged by size:
1106 184 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 599 684 1190 1419
Size: 599 85 506 229
Fragments arranged by size:
599 506 229 85
BsaBI GATnn'nnATC
Cuts at: 0 371 933 1419
Size: 371 562 486
Fragments arranged by size:
562 486 371
BsaWI w'CCGG_w
Cuts at: 0 1316 1419
Size: 1316 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 746 776 1419
Size: 746 30 643
Fragments arranged by size:
746 643 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 710 1178 1419
Size: 710 468 241
Fragments arranged by size:
710 468 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 641 653 1001 1419
Size: 596 45 12 348 418
Fragments arranged by size:
596 418 348 45 12
BsiEI CG_ry'CG
Cuts at: 0 1305 1419
Size: 1305 114
BsiWI C'GTAC_G
Cuts at: 0 1289 1419
Size: 1289 130
BsmBI CGTCTCn'nnnn_
Cuts at: 0 250 1419
Size: 250 1169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 568 833 1419
Size: 568 265 586
Fragments arranged by size:
586 568 265
BsrGI T'GTAC_A
Cuts at: 0 467 1419
Size: 467 952
BssSI C'ACGA_G
Cuts at: 0 1240 1419
Size: 1240 179
BstYI r'GATC_y
Cuts at: 0 759 1252 1419
Size: 759 493 167
Fragments arranged by size:
759 493 167
EarI CTCTTCn'nnn_
Cuts at: 0 1028 1419
Size: 1028 391
EcoO109I rG'GnC_Cy
Cuts at: 0 273 1419
Size: 273 1146
EcoRI G'AATT_C
Cuts at: 0 1324 1419
Size: 1324 95
EcoRV GAT'ATC
Cuts at: 0 931 1419
Size: 931 488
HaeII r_GCGC'y
Cuts at: 0 1022 1419
Size: 1022 397
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 598 630 976 1008 1119 1151 1419
Size: 598 32 346 32 111 32 268
Fragments arranged by size:
598 346 268 111 32 32 32
HincII GTy'rAC
Cuts at: 0 822 1263 1419
Size: 822 441 156
Fragments arranged by size:
822 441 156
KpnI G_GTAC'C
Cuts at: 0 962 1419
Size: 962 457
MslI CAynn'nnrTG
Cuts at: 0 181 442 1419
Size: 181 261 977
Fragments arranged by size:
977 261 181
MspA1I CmG'CkG
Cuts at: 0 1277 1419
Size: 1277 142
NdeI CA'TA_TG
Cuts at: 0 954 1419
Size: 954 465
NspI r_CATG'y
Cuts at: 0 1074 1395 1419
Size: 1074 321 24
Fragments arranged by size:
1074 321 24
PstI C_TGCA'G
Cuts at: 0 533 1419
Size: 533 886
PvuII CAG'CTG
Cuts at: 0 1277 1419
Size: 1277 142
SalI G'TCGA_C
Cuts at: 0 820 1419
Size: 820 599
SapI GCTCTTCn'nnn_
Cuts at: 0 1028 1419
Size: 1028 391
ScaI AGT'ACT
Cuts at: 0 967 1419
Size: 967 452
SexAI A'CCwGG_T
Cuts at: 0 666 684 1419
Size: 666 18 735
Fragments arranged by size:
735 666 18
SfcI C'TryA_G
Cuts at: 0 529 1278 1419
Size: 529 749 141
Fragments arranged by size:
749 529 141
SmlI C'TyrA_G
Cuts at: 0 199 1269 1419
Size: 199 1070 150
Fragments arranged by size:
1070 199 150
SphI G_CATG'C
Cuts at: 0 1395 1419
Size: 1395 24
StyI C'CwwG_G
Cuts at: 0 1029 1419
Size: 1029 390
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 589 1319 1419
Size: 589 730 100
Fragments arranged by size:
730 589 100
TatI w'GTAC_w
Cuts at: 0 203 212 347 467 965 1419
Size: 203 9 135 120 498 454
Fragments arranged by size:
498 454 203 135 120 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 1336 1419
Size: 180 1156 83
Fragments arranged by size:
1156 180 83
XmnI GAAnn'nnTTC
Cuts at: 0 1324 1419
Size: 1324 95
Enzymes that do cut:
AccI Acc65I AfeI AhdI AlwNI ApoI BanI BclI
BglI BglII BlpI BmgBI BmrI BpuEI BsaI BsaBI
BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspMI
BsrGI BssSI BstYI EarI EcoO109I EcoRI EcoRV HaeII
Hin4I HincII KpnI MslI MspA1I NdeI NspI PstI
PvuII SalI SapI ScaI SexAI SfcI SmlI SphI
StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AflIII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BfrBI Bme1580I BmtI BplI BpmI Bpu10I BsaAI
BsaHI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI
BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI FalI
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
MmeI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SanDI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI