Restriction map for
pF1KB4011
(Linear) (Six-base) MAPSORT of: KIBB4011.seq Check: 82 from: 1 to: 1653
KIBB4011.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 140 1653
Size: 140 1513
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 322 427 649 743 823 1210 1653
Size: 262 60 105 222 94 80 387 443
Fragments arranged by size:
443 387 262 222 105 94 80 60
AfeI AGC'GCT
Cuts at: 0 1105 1653
Size: 1105 548
AflIII A'CryG_T
Cuts at: 0 606 1653
Size: 606 1047
AleI CACnn'nnGTG
Cuts at: 0 1235 1653
Size: 1235 418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 385 1653
Size: 385 1268
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 952 984 1653
Size: 952 32 669
Fragments arranged by size:
952 669 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1089 1248 1653
Size: 1089 159 405
Fragments arranged by size:
1089 405 159
ApaI G_GGCC'C
Cuts at: 0 35 1653
Size: 35 1618
ApaLI G'TGCA_C
Cuts at: 0 153 1534 1653
Size: 153 1381 119
Fragments arranged by size:
1381 153 119
AvaI C'yCGr_G
Cuts at: 0 412 1653
Size: 412 1241
BamHI G'GATC_C
Cuts at: 0 128 840 1653
Size: 128 712 813
Fragments arranged by size:
813 712 128
BanI G'GyrC_C
Cuts at: 0 775 1229 1335 1653
Size: 775 454 106 318
Fragments arranged by size:
775 454 318 106
BanII G_rGCy'C
Cuts at: 0 35 122 314 523 1653
Size: 35 87 192 209 1130
Fragments arranged by size:
1130 209 192 87 35
BbeI G_GCGC'C
Cuts at: 0 1339 1653
Size: 1339 314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 996 1030 1653
Size: 996 34 623
Fragments arranged by size:
996 623 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1075 1653
Size: 1075 578
BlpI GC'TnA_GC
Cuts at: 0 389 1653
Size: 389 1264
Bme1580I G_kGCm'C
Cuts at: 0 35 157 549 769 1298 1538 1653
Size: 35 122 392 220 529 240 115
Fragments arranged by size:
529 392 240 220 122 115 35
BmgBI CAC'GTC
Cuts at: 0 537 1653
Size: 537 1116
BmrI ACTGGGnnnn_n'
Cuts at: 0 559 1653
Size: 559 1094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 1528 1653
Size: 103 1425 125
Fragments arranged by size:
1425 125 103
Bpu10I CC'TnA_GC
Cuts at: 0 54 1156 1653
Size: 54 1102 497
Fragments arranged by size:
1102 497 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 4 1653
Size: 4 1649
BsaAI yAC'GTr
Cuts at: 0 609 1653
Size: 609 1044
BsaHI Gr'CG_yC
Cuts at: 0 571 1336 1653
Size: 571 765 317
Fragments arranged by size:
765 571 317
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 109 139 1653
Size: 76 30 3 30 1514
Fragments arranged by size:
1514 76 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 886 1000 1210 1653
Size: 886 114 210 443
Fragments arranged by size:
886 443 210 114
BseYI C'CCAG_C
Cuts at: 0 165 1248 1485 1653
Size: 165 1083 237 168
Fragments arranged by size:
1083 237 168 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1438 1653
Size: 1438 215
BsiHKAI G_wGCw'C
Cuts at: 0 157 314 523 1277 1538 1653
Size: 157 157 209 754 261 115
Fragments arranged by size:
754 261 209 157 157 115
Bsp1286I G_dGCh'C
Cuts at: 0 35 122 157 314 523 549 769 1277
Size: 35 87 35 157 209 26 220 508
Cuts at: 1277 1298 1538 1653
Size: 21 240 115
Fragments arranged by size:
508 240 220 209 157 115 87 35
35 26 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 140 1653
Size: 140 1513
BsrBI CCG'CTC
Cuts at: 0 53 1641 1653
Size: 53 1588 12
Fragments arranged by size:
1588 53 12
BsrDI GCAATG_nn'
Cuts at: 0 788 1653
Size: 788 865
BsrFI r'CCGG_y
Cuts at: 0 170 1653
Size: 170 1483
BssSI C'ACGA_G
Cuts at: 0 1537 1653
Size: 1537 116
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1089 1653
Size: 1089 564
BstEII G'GTnAC_C
Cuts at: 0 1599 1653
Size: 1599 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 942 1653
Size: 942 711
BstYI r'GATC_y
Cuts at: 0 128 716 840 1307 1466 1544 1653
Size: 128 588 124 467 159 78 109
Fragments arranged by size:
588 467 159 128 124 109 78
BtgI C'CryG_G
Cuts at: 0 935 1340 1653
Size: 935 405 313
Fragments arranged by size:
935 405 313
BtsI GCAGTG_nn'
Cuts at: 0 200 482 733 1050 1653
Size: 200 282 251 317 603
Fragments arranged by size:
603 317 282 251 200
EaeI y'GGCC_r
Cuts at: 0 317 1067 1628 1653
Size: 317 750 561 25
Fragments arranged by size:
750 561 317 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1459 1653
Size: 1459 194
EcoICRI GAG'CTC
Cuts at: 0 312 521 1653
Size: 312 209 1132
Fragments arranged by size:
1132 312 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 262 322 427 649 743 823 1210
Size: 103 159 60 105 222 94 80 387
Cuts at: 1210 1528 1653
Size: 318 125
Fragments arranged by size:
387 318 222 159 125 105 103 94
80 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 85 1653
Size: 85 1568
EcoO109I rG'GnC_Cy
Cuts at: 0 31 92 1200 1653
Size: 31 61 1108 453
Fragments arranged by size:
1108 453 61 31
FseI GG_CCGG'CC
Cuts at: 0 174 1653
Size: 174 1479
FspI TGC'GCA
Cuts at: 0 1082 1653
Size: 1082 571
HaeII r_GCGC'y
Cuts at: 0 1107 1339 1653
Size: 1107 232 314
Fragments arranged by size:
1107 314 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 421 453 471 503 812 844
Size: 76 32 313 32 18 32 309 32
Cuts at: 844 1653
Size: 809
Fragments arranged by size:
809 313 309 76 32 32 32 32
18
KasI G'GCGC_C
Cuts at: 0 1335 1653
Size: 1335 318
MscI TGG'CCA
Cuts at: 0 319 1653
Size: 319 1334
MslI CAynn'nnrTG
Cuts at: 0 262 499 502 751 835 1235 1653
Size: 262 237 3 249 84 400 418
Fragments arranged by size:
418 400 262 249 237 84 3
MspA1I CmG'CkG
Cuts at: 0 165 709 1266 1578 1653
Size: 165 544 557 312 75
Fragments arranged by size:
557 544 312 165 75
NaeI GCC'GGC
Cuts at: 0 172 1653
Size: 172 1481
NarI GG'CG_CC
Cuts at: 0 1336 1653
Size: 1336 317
NgoMIV G'CCGG_C
Cuts at: 0 170 1653
Size: 170 1483
NspI r_CATG'y
Cuts at: 0 750 1011 1392 1653
Size: 750 261 381 261
Fragments arranged by size:
750 381 261 261
PfoI T'CCnGG_A
Cuts at: 0 439 816 1313 1505 1653
Size: 439 377 497 192 148
Fragments arranged by size:
497 439 377 192 148
PmlI CAC'GTG
Cuts at: 0 609 1653
Size: 609 1044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 976 1008 1378 1410 1653
Size: 976 32 370 32 243
Fragments arranged by size:
976 370 243 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 92 1200 1653
Size: 92 1108 453
Fragments arranged by size:
1108 453 92
PspOMI G'GGCC_C
Cuts at: 0 31 1653
Size: 31 1622
PstI C_TGCA'G
Cuts at: 0 725 1094 1178 1653
Size: 725 369 84 475
Fragments arranged by size:
725 475 369 84
PvuII CAG'CTG
Cuts at: 0 165 1653
Size: 165 1488
SacI G_AGCT'C
Cuts at: 0 314 523 1653
Size: 314 209 1130
Fragments arranged by size:
1130 314 209
SanDI GG'GwC_CC
Cuts at: 0 92 1653
Size: 92 1561
SfcI C'TryA_G
Cuts at: 0 721 1090 1174 1653
Size: 721 369 84 479
Fragments arranged by size:
721 479 369 84
SfoI GGC'GCC
Cuts at: 0 1337 1653
Size: 1337 316
SmaI CCC'GGG
Cuts at: 0 414 1653
Size: 414 1239
SphI G_CATG'C
Cuts at: 0 1011 1653
Size: 1011 642
StyI C'CwwG_G
Cuts at: 0 113 1653
Size: 113 1540
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 109 141 1653
Size: 109 32 1512
Fragments arranged by size:
1512 109 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 684 1653
Size: 684 969
XmaI C'CCGG_G
Cuts at: 0 412 1653
Size: 412 1241
XmnI GAAnn'nnTTC
Cuts at: 0 292 1324 1653
Size: 292 1032 329
Fragments arranged by size:
1032 329 292
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI BtgI BtsI EaeI EciI EcoICRI Eco57MI EcoNI
EcoO109I FseI FspI HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PfoI PmlI PpiI
PpuMI PspOMI PstI PvuII SacI SanDI SfcI SfoI
SmaI SphI StyI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI ApoI
AscI AseI AsiSI AvrII BaeI BbsI BbvCI BciVI
BclI BfrBI BglII BmtI BplI BpuEI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EcoRI EcoRV FalI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TatI Tth111I XbaI XhoI ZraI