Restriction map for
pF1KB3946
(Linear) (Six-base) MAPSORT of: KIBB3946.seq Check: 6405 from: 1 to: 1527
KIBB3946.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AatII G_ACGT'C
Cuts at: 0 845 1527
Size: 845 682
AccI GT'mk_AC
Cuts at: 0 1505 1527
Size: 1505 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 775 1527
Size: 199 576 752
Fragments arranged by size:
752 576 199
AflIII A'CryG_T
Cuts at: 0 152 998 1527
Size: 152 846 529
Fragments arranged by size:
846 529 152
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 139 171 187 219 1330 1362 1527
Size: 139 32 16 32 1111 32 165
Fragments arranged by size:
1111 165 139 32 32 32 16
AlwNI CAG_nnn'CTG
Cuts at: 0 891 1527
Size: 891 636
ApaI G_GGCC'C
Cuts at: 0 700 1527
Size: 700 827
ApaLI G'TGCA_C
Cuts at: 0 661 1527
Size: 661 866
AvaI C'yCGr_G
Cuts at: 0 681 909 1527
Size: 681 228 618
Fragments arranged by size:
681 618 228
BanI G'GyrC_C
Cuts at: 0 58 264 767 915 1020 1071 1092 1113
Size: 58 206 503 148 105 51 21 21
Cuts at: 1113 1375 1527
Size: 262 152
Fragments arranged by size:
503 262 206 152 148 105 58 51
21 21
BanII G_rGCy'C
Cuts at: 0 97 115 173 404 700 1210 1527
Size: 97 18 58 231 296 510 317
Fragments arranged by size:
510 317 296 231 97 58 18
BbeI G_GCGC'C
Cuts at: 0 62 771 1024 1075 1096 1527
Size: 62 709 253 51 21 431
Fragments arranged by size:
709 431 253 62 51 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 348 382 1527
Size: 348 34 1145
Fragments arranged by size:
1145 348 34
BglI GCCn_nnn'nGGC
Cuts at: 0 56 110 982 1060 1069 1527
Size: 56 54 872 78 9 458
Fragments arranged by size:
872 458 78 56 54 9
BlpI GC'TnA_GC
Cuts at: 0 403 832 1153 1204 1415 1527
Size: 403 429 321 51 211 112
Fragments arranged by size:
429 403 321 211 112 51
Bme1580I G_kGCm'C
Cuts at: 0 269 665 700 1378 1527
Size: 269 396 35 678 149
Fragments arranged by size:
678 396 269 149 35
BmgBI CAC'GTC
Cuts at: 0 312 732 816 1527
Size: 312 420 84 711
Fragments arranged by size:
711 420 312 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 1500 1527
Size: 1500 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 681 1527
Size: 681 846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 447 1326 1527
Size: 276 171 879 201
Fragments arranged by size:
879 276 201 171
BsaI GGTCTCn'nnnn_
Cuts at: 0 853 1527
Size: 853 674
BsaAI yAC'GTr
Cuts at: 0 999 1527
Size: 999 528
BsaHI Gr'CG_yC
Cuts at: 0 59 768 842 1021 1072 1093 1527
Size: 59 709 74 179 51 21 434
Fragments arranged by size:
709 434 179 74 59 51 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 187 217 628 658 832 862
Size: 139 30 18 30 411 30 174 30
Cuts at: 862 1273 1303 1324 1330 1354 1360 1527
Size: 411 30 21 6 24 6 167
Fragments arranged by size:
411 411 174 167 139 30 30 30
30 30 24 21 18 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 481 583 630 633 1527
Size: 208 273 102 47 3 894
Fragments arranged by size:
894 273 208 102 47 3
BseYI C'CCAG_C
Cuts at: 0 64 414 1175 1210 1484 1527
Size: 64 350 761 35 274 43
Fragments arranged by size:
761 350 274 64 43 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 754 1527
Size: 508 246 773
Fragments arranged by size:
773 508 246
BsiHKAI G_wGCw'C
Cuts at: 0 242 404 665 680 1527
Size: 242 162 261 15 847
Fragments arranged by size:
847 261 242 162 15
Bsp1286I G_dGCh'C
Cuts at: 0 97 115 173 242 269 404 665 680
Size: 97 18 58 69 27 135 261 15
Cuts at: 680 700 1210 1378 1527
Size: 20 510 168 149
Fragments arranged by size:
510 261 168 149 135 97 69 58
27 20 18 15
BspHI T'CATG_A
Cuts at: 0 20 1527
Size: 20 1507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 899 1527
Size: 899 628
BsrBI CCG'CTC
Cuts at: 0 477 1310 1414 1527
Size: 477 833 104 113
Fragments arranged by size:
833 477 113 104
BsrFI r'CCGG_y
Cuts at: 0 273 918 1059 1107 1378 1527
Size: 273 645 141 48 271 149
Fragments arranged by size:
645 273 271 149 141 48
BsrGI T'GTAC_A
Cuts at: 0 1427 1527
Size: 1427 100
BssHII G'CGCG_C
Cuts at: 0 355 1527
Size: 355 1172
BstEII G'GTnAC_C
Cuts at: 0 936 1527
Size: 936 591
BtgI C'CryG_G
Cuts at: 0 89 526 575 971 1527
Size: 89 437 49 396 556
Fragments arranged by size:
556 437 396 89 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1422 1527
Size: 1422 105
EaeI y'GGCC_r
Cuts at: 0 903 920 930 974 1527
Size: 903 17 10 44 553
Fragments arranged by size:
903 553 44 17 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 487 546 574 1527
Size: 487 59 28 953
Fragments arranged by size:
953 487 59 28
EcoICRI GAG'CTC
Cuts at: 0 402 1527
Size: 402 1125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 276 447 775 1326 1527
Size: 199 77 171 328 551 201
Fragments arranged by size:
551 328 201 199 171 77
EcoO109I rG'GnC_Cy
Cuts at: 0 48 696 1053 1455 1527
Size: 48 648 357 402 72
Fragments arranged by size:
648 402 357 72 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 474 1527
Size: 474 1053
HaeII r_GCGC'y
Cuts at: 0 62 619 771 1024 1075 1096 1422 1527
Size: 62 557 152 253 51 21 326 105
Fragments arranged by size:
557 326 253 152 105 62 51 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 832 864 1527
Size: 832 32 663
Fragments arranged by size:
832 663 32
HincII GTy'rAC
Cuts at: 0 285 873 1527
Size: 285 588 654
Fragments arranged by size:
654 588 285
KasI G'GCGC_C
Cuts at: 0 58 767 1020 1071 1092 1527
Size: 58 709 253 51 21 435
Fragments arranged by size:
709 435 253 58 51 21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1527
Size: 875 652
MslI CAynn'nnrTG
Cuts at: 0 25 274 1402 1527
Size: 25 249 1128 125
Fragments arranged by size:
1128 249 125 25
MspA1I CmG'CkG
Cuts at: 0 91 528 973 1203 1527
Size: 91 437 445 230 324
Fragments arranged by size:
445 437 324 230 91
NaeI GCC'GGC
Cuts at: 0 920 1061 1109 1527
Size: 920 141 48 418
Fragments arranged by size:
920 418 141 48
NarI GG'CG_CC
Cuts at: 0 59 768 1021 1072 1093 1527
Size: 59 709 253 51 21 434
Fragments arranged by size:
709 434 253 59 51 21
NgoMIV G'CCGG_C
Cuts at: 0 918 1059 1107 1527
Size: 918 141 48 420
Fragments arranged by size:
918 420 141 48
PasI CC'CwG_GG
Cuts at: 0 693 1527
Size: 693 834
PfoI T'CCnGG_A
Cuts at: 0 8 1527
Size: 8 1519
PmlI CAC'GTG
Cuts at: 0 999 1527
Size: 999 528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 187 219 1330 1362 1527
Size: 139 32 16 32 1111 32 165
Fragments arranged by size:
1111 165 139 32 32 32 16
PpuMI rG'GwC_Cy
Cuts at: 0 1455 1527
Size: 1455 72
PspOMI G'GGCC_C
Cuts at: 0 696 1527
Size: 696 831
PstI C_TGCA'G
Cuts at: 0 623 1527
Size: 623 904
PvuII CAG'CTG
Cuts at: 0 1203 1527
Size: 1203 324
SacI G_AGCT'C
Cuts at: 0 404 1527
Size: 404 1123
SacII CC_GC'GG
Cuts at: 0 92 529 974 1527
Size: 92 437 445 553
Fragments arranged by size:
553 445 437 92
SanDI GG'GwC_CC
Cuts at: 0 1455 1527
Size: 1455 72
SfcI C'TryA_G
Cuts at: 0 619 1218 1257 1281 1353 1527
Size: 619 599 39 24 72 174
Fragments arranged by size:
619 599 174 72 39 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1060 1527
Size: 1060 467
SfoI GGC'GCC
Cuts at: 0 60 769 1022 1073 1094 1527
Size: 60 709 253 51 21 433
Fragments arranged by size:
709 433 253 60 51 21
SmaI CCC'GGG
Cuts at: 0 911 1527
Size: 911 616
StyI C'CwwG_G
Cuts at: 0 161 1527
Size: 161 1366
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 726 1527
Size: 46 680 801
Fragments arranged by size:
801 680 46
TatI w'GTAC_w
Cuts at: 0 1427 1527
Size: 1427 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 628 660 1324 1356 1527
Size: 628 32 664 32 171
Fragments arranged by size:
664 628 171 32 32
XmaI C'CCGG_G
Cuts at: 0 909 1527
Size: 909 618
XmnI GAAnn'nnTTC
Cuts at: 0 154 331 1527
Size: 154 177 1196
Fragments arranged by size:
1196 177 154
ZraI GAC'GTC
Cuts at: 0 843 1527
Size: 843 684
Enzymes that do cut:
AatII AccI AcuI AflIII AloI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BcgI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrGI BssHII BstEII BtgI BtgZI EaeI EciI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI
MmeI MslI MspA1I NaeI NarI NgoMIV PasI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII
SanDI SfcI SfiI SfoI SmaI StyI TaqII TatI
TstI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BciVI BclI BfrBI BglII BmtI Bpu10I
BpuEI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BsrDI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MscI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI
ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI