(Linear) (Six-base) MAPSORT of: KIBB3940.seq  Check: 5022  from: 1  to: 513



KIBB3940.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:      0     428     513 
   Size:        428      85
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     513 
   Size:        102     411
 
AleI CACnn'nnGTG

Cuts at:      0     415     513 
   Size:        415      98
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     433     513 
   Size:        433      80
 
AlwNI CAG_nnn'CTG

Cuts at:      0     129     138     279     513 
   Size:        129       9     141     234

  Fragments arranged by size: 

                 234     141     129       9
 
AvaI C'yCGr_G

Cuts at:      0      17     513 
   Size:         17     496
 
BamHI G'GATC_C

Cuts at:      0     228     513 
   Size:        228     285
 
BanII G_rGCy'C

Cuts at:      0      92     478     513 
   Size:         92     386      35

  Fragments arranged by size: 

                 386      92      35
 
BfrBI ATG'CAT

Cuts at:      0       3     513 
   Size:          3     510
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     372     513 
   Size:        372     141
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     478     513 
   Size:         92     386      35

  Fragments arranged by size: 

                 386      92      35
 
BssSI C'ACGA_G

Cuts at:      0      93     513 
   Size:         93     420
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     138     513 
   Size:        138     375
 
BstEII G'GTnAC_C

Cuts at:      0     196     513 
   Size:        196     317
 
BstYI r'GATC_y

Cuts at:      0     228     513 
   Size:        228     285
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     269     513 
   Size:        269     244
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     513 
   Size:        102     411
 
HincII GTy'rAC

Cuts at:      0     318     513 
   Size:        318     195
 
MslI CAynn'nnrTG

Cuts at:      0     364     415     513 
   Size:        364      51      98

  Fragments arranged by size: 

                 364      98      51
 
MspA1I CmG'CkG

Cuts at:      0     108     255     443     513 
   Size:        108     147     188      70

  Fragments arranged by size: 

                 188     147     108      70
 
NsiI A_TGCA'T

Cuts at:      0       5     513 
   Size:          5     508
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     225     513 
   Size:        225     288
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     293     325     513 
   Size:        293      32     188

  Fragments arranged by size: 

                 293     188      32
 
PsiI TTA'TAA

Cuts at:      0     155     513 
   Size:        155     358
 
PstI C_TGCA'G

Cuts at:      0     113     134     513 
   Size:        113      21     379

  Fragments arranged by size: 

                 379     113      21
 
PvuII CAG'CTG

Cuts at:      0     108     443     513 
   Size:        108     335      70

  Fragments arranged by size: 

                 335     108      70
 
SbfI CC_TGCA'GG

Cuts at:      0     134     513 
   Size:        134     379
 
SfcI C'TryA_G

Cuts at:      0     109     130     336     513 
   Size:        109      21     206     177

  Fragments arranged by size: 

                 206     177     109      21
 
SmaI CCC'GGG

Cuts at:      0      19     513 
   Size:         19     494
 
StyI C'CwwG_G

Cuts at:      0      83     513 
   Size:         83     430
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     420     513 
   Size:        420      93
 
XmaI C'CCGG_G

Cuts at:      0      17     513 
   Size:         17     496
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AlfI    AlwNI     AvaI    BamHI    BanII
    BfrBI     BplI Bsp1286I    BssSI   BstAPI   BstEII    BstYI     DrdI
  Eco57MI   HincII     MslI   MspA1I     NsiI    PflMI     PpiI     PsiI
     PstI    PvuII     SbfI     SfcI     SmaI     StyI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmnI     ZraI