Restriction map for
pF1KB3929
(Linear) (Six-base) MAPSORT of: KIBB3929.seq Check: 5512 from: 1 to: 774
KIBB3929.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 774
Size: 667 107
AlwNI CAG_nnn'CTG
Cuts at: 0 523 774
Size: 523 251
ApaI G_GGCC'C
Cuts at: 0 104 774
Size: 104 670
AvaI C'yCGr_G
Cuts at: 0 83 774
Size: 83 691
BanI G'GyrC_C
Cuts at: 0 55 406 774
Size: 55 351 368
Fragments arranged by size:
368 351 55
BanII G_rGCy'C
Cuts at: 0 104 242 774
Size: 104 138 532
Fragments arranged by size:
532 138 104
BbeI G_GCGC'C
Cuts at: 0 59 774
Size: 59 715
BbvCI CC'TCA_GC
Cuts at: 0 158 774
Size: 158 616
BglI GCCn_nnn'nGGC
Cuts at: 0 64 774
Size: 64 710
Bme1580I G_kGCm'C
Cuts at: 0 104 774
Size: 104 670
BmrI ACTGGGnnnn_n'
Cuts at: 0 646 774
Size: 646 128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 774
Size: 283 491
Bpu10I CC'TnA_GC
Cuts at: 0 158 774
Size: 158 616
BsaI GGTCTCn'nnnn_
Cuts at: 0 80 379 774
Size: 80 299 395
Fragments arranged by size:
395 299 80
BsaHI Gr'CG_yC
Cuts at: 0 56 80 774
Size: 56 24 694
Fragments arranged by size:
694 56 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 269 697 774
Size: 250 19 428 77
Fragments arranged by size:
428 250 77 19
BseYI C'CCAG_C
Cuts at: 0 419 774
Size: 419 355
BsiEI CG_ry'CG
Cuts at: 0 64 170 774
Size: 64 106 604
Fragments arranged by size:
604 106 64
BsiHKAI G_wGCw'C
Cuts at: 0 242 774
Size: 242 532
Bsp1286I G_dGCh'C
Cuts at: 0 104 242 774
Size: 104 138 532
Fragments arranged by size:
532 138 104
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 774
Size: 173 601
BsrFI r'CCGG_y
Cuts at: 0 63 704 774
Size: 63 641 70
Fragments arranged by size:
641 70 63
BstAPI GCAn_nnn'nTGC
Cuts at: 0 132 774
Size: 132 642
BstXI CCAn_nnnn'nTGG
Cuts at: 0 636 774
Size: 636 138
BtgI C'CryG_G
Cuts at: 0 113 164 629 774
Size: 113 51 465 145
Fragments arranged by size:
465 145 113 51
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 701 736 774
Size: 701 35 38
Fragments arranged by size:
701 38 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 641 774
Size: 641 133
EaeI y'GGCC_r
Cuts at: 0 61 167 774
Size: 61 106 607
Fragments arranged by size:
607 106 61
EagI C'GGCC_G
Cuts at: 0 61 167 774
Size: 61 106 607
Fragments arranged by size:
607 106 61
EarI CTCTTCn'nnn_
Cuts at: 0 234 774
Size: 234 540
EcoICRI GAG'CTC
Cuts at: 0 240 774
Size: 240 534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 667 774
Size: 283 384 107
Fragments arranged by size:
384 283 107
EcoO109I rG'GnC_Cy
Cuts at: 0 144 185 774
Size: 144 41 589
Fragments arranged by size:
589 144 41
FseI GG_CCGG'CC
Cuts at: 0 67 774
Size: 67 707
HaeII r_GCGC'y
Cuts at: 0 59 774
Size: 59 715
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 610 642 774
Size: 610 32 132
Fragments arranged by size:
610 132 32
HincII GTy'rAC
Cuts at: 0 738 774
Size: 738 36
KasI G'GCGC_C
Cuts at: 0 55 774
Size: 55 719
MspA1I CmG'CkG
Cuts at: 0 115 774
Size: 115 659
NaeI GCC'GGC
Cuts at: 0 65 774
Size: 65 709
NarI GG'CG_CC
Cuts at: 0 56 774
Size: 56 718
NcoI C'CATG_G
Cuts at: 0 629 774
Size: 629 145
NgoMIV G'CCGG_C
Cuts at: 0 63 774
Size: 63 711
PasI CC'CwG_GG
Cuts at: 0 660 774
Size: 660 114
PfoI T'CCnGG_A
Cuts at: 0 260 774
Size: 260 514
PpuMI rG'GwC_Cy
Cuts at: 0 144 185 774
Size: 144 41 589
Fragments arranged by size:
589 144 41
PspOMI G'GGCC_C
Cuts at: 0 100 774
Size: 100 674
SacI G_AGCT'C
Cuts at: 0 242 774
Size: 242 532
SacII CC_GC'GG
Cuts at: 0 116 774
Size: 116 658
ScaI AGT'ACT
Cuts at: 0 130 555 774
Size: 130 425 219
Fragments arranged by size:
425 219 130
SfoI GGC'GCC
Cuts at: 0 57 774
Size: 57 717
StyI C'CwwG_G
Cuts at: 0 70 629 774
Size: 70 559 145
Fragments arranged by size:
559 145 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 506 774
Size: 506 268
TatI w'GTAC_w
Cuts at: 0 128 451 553 774
Size: 128 323 102 221
Fragments arranged by size:
323 221 128 102
Tth111I GACn'n_nGTC
Cuts at: 0 523 638 774
Size: 523 115 136
Fragments arranged by size:
523 136 115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 186 774
Size: 186 588
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BbeI BbvCI
BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstXI
BtgI CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I FseI HaeII Hin4I HincII KasI MspA1I NaeI
NarI NcoI NgoMIV PasI PfoI PpuMI PspOMI SacI
SacII ScaI SfoI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI DraI
DraIII EciI EcoNI EcoRI EcoRV FalI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SalI SanDI SapI SbfI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XhoI XmaI XmnI ZraI