(Linear) (Six-base) MAPSORT of: KIBB3876.seq  Check: 7721  from: 1  to: 495



KIBB3876.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

Acc65I G'GTAC_C

Cuts at:      0     206     495 
   Size:        206     289
 
AlwNI CAG_nnn'CTG

Cuts at:      0     301     495 
   Size:        301     194
 
ApoI r'AATT_y

Cuts at:      0     445     495 
   Size:        445      50
 
AvaI C'yCGr_G

Cuts at:      0     161     495 
   Size:        161     334
 
BanI G'GyrC_C

Cuts at:      0     206     403     495 
   Size:        206     197      92

  Fragments arranged by size: 

                 206     197      92
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     235     495 
   Size:        235     260
 
Bme1580I G_kGCm'C

Cuts at:      0     122     495 
   Size:        122     373
 
BmgBI CAC'GTC

Cuts at:      0     478     495 
   Size:        478      17
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     346     495 
   Size:        210     136     149

  Fragments arranged by size: 

                 210     149     136
 
Bpu10I CC'TnA_GC

Cuts at:      0      89     495 
   Size:         89     406
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     495 
   Size:        139     356
 
BsaAI yAC'GTr

Cuts at:      0     171     495 
   Size:        171     324
 
BsaWI w'CCGG_w

Cuts at:      0      20     495 
   Size:         20     475
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      47      77     495 
   Size:         47      30     418

  Fragments arranged by size: 

                 418      47      30
 
BseYI C'CCAG_C

Cuts at:      0     424     430     495 
   Size:        424       6      65

  Fragments arranged by size: 

                 424      65       6
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     495 
   Size:        122     373
 
BspHI T'CATG_A

Cuts at:      0     134     495 
   Size:        134     361
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     348     495 
   Size:        348     147
 
DraIII CAC_nnn'GTG

Cuts at:      0     403     495 
   Size:        403      92
 
EaeI y'GGCC_r

Cuts at:      0      65     495 
   Size:         65     430
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     476     495 
   Size:        476      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     346     495 
   Size:        210     136     149

  Fragments arranged by size: 

                 210     149     136
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      46     495 
   Size:         46     449
 
EcoRI G'AATT_C

Cuts at:      0     445     495 
   Size:        445      50
 
KpnI G_GTAC'C

Cuts at:      0     210     495 
   Size:        210     285
 
MscI TGG'CCA

Cuts at:      0      67     495 
   Size:         67     428
 
NspI r_CATG'y

Cuts at:      0      87     495 
   Size:         87     408
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     301     495 
   Size:        301     194
 
SexAI A'CCwGG_T

Cuts at:      0     399     495 
   Size:        399      96
 
SfcI C'TryA_G

Cuts at:      0     378     495 
   Size:        378     117
 
SmlI C'TyrA_G

Cuts at:      0     154     495 
   Size:        154     341
 
StuI AGG'CCT

Cuts at:      0     309     495 
   Size:        309     186
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      47      79     495 
   Size:         47      32     416

  Fragments arranged by size: 

                 416      47      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     479     495 
   Size:        479      16
 

 Enzymes that do cut:

   Acc65I    AlwNI     ApoI     AvaI     BanI    BciVI Bme1580I    BmgBI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI    BseYI Bsp1286I
    BspHI    BspMI   DraIII     EaeI     EciI  Eco57MI    EcoNI    EcoRI
     KpnI     MscI     NspI    PflMI    SexAI     SfcI     SmlI     StuI
     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI     BsaI    BsaBI    BsaHI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EagI     EarI  EcoICRI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI    TaqII     TatI     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI