Restriction map for
pF1KB3870
(Linear) (Six-base) MAPSORT of: KIBB3870.seq Check: 4615 from: 1 to: 1101
KIBB3870.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AccI GT'mk_AC
Cuts at: 0 266 1101
Size: 266 835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 838 886 1101
Size: 305 533 48 215
Fragments arranged by size:
533 305 215 48
AflIII A'CryG_T
Cuts at: 0 653 1101
Size: 653 448
AlwNI CAG_nnn'CTG
Cuts at: 0 816 1101
Size: 816 285
AvaI C'yCGr_G
Cuts at: 0 61 1101
Size: 61 1040
BanI G'GyrC_C
Cuts at: 0 715 848 929 1101
Size: 715 133 81 172
Fragments arranged by size:
715 172 133 81
BanII G_rGCy'C
Cuts at: 0 25 58 574 1101
Size: 25 33 516 527
Fragments arranged by size:
527 516 33 25
BbeI G_GCGC'C
Cuts at: 0 719 852 1101
Size: 719 133 249
Fragments arranged by size:
719 249 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 1025 1101
Size: 1025 76
BciVI GTATCCnnnnn_n'
Cuts at: 0 14 1101
Size: 14 1087
BglI GCCn_nnn'nGGC
Cuts at: 0 280 1101
Size: 280 821
BglII A'GATC_T
Cuts at: 0 353 1101
Size: 353 748
Bme1580I G_kGCm'C
Cuts at: 0 559 1101
Size: 559 542
BmgBI CAC'GTC
Cuts at: 0 525 560 1101
Size: 525 35 541
Fragments arranged by size:
541 525 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 304 561 1101
Size: 304 257 540
Fragments arranged by size:
540 304 257
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 612 1101
Size: 612 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 451 466 588 1101
Size: 91 360 15 122 513
Fragments arranged by size:
513 360 122 91 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 771 1101
Size: 771 330
BsaI GGTCTCn'nnnn_
Cuts at: 0 150 496 1101
Size: 150 346 605
Fragments arranged by size:
605 346 150
BsaAI yAC'GTr
Cuts at: 0 453 1101
Size: 453 648
BsaHI Gr'CG_yC
Cuts at: 0 716 849 1101
Size: 716 133 252
Fragments arranged by size:
716 252 133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 853 1101
Size: 853 248
BseYI C'CCAG_C
Cuts at: 0 911 1101
Size: 911 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 885 1101
Size: 700 185 216
Fragments arranged by size:
700 216 185
BsiHKAI G_wGCw'C
Cuts at: 0 574 962 1101
Size: 574 388 139
Fragments arranged by size:
574 388 139
BsmBI CGTCTCn'nnnn_
Cuts at: 0 370 1101
Size: 370 731
Bsp1286I G_dGCh'C
Cuts at: 0 25 58 559 574 962 1101
Size: 25 33 501 15 388 139
Fragments arranged by size:
501 388 139 33 25 15
BsrBI CCG'CTC
Cuts at: 0 384 1101
Size: 384 717
BsrFI r'CCGG_y
Cuts at: 0 274 845 1101
Size: 274 571 256
Fragments arranged by size:
571 274 256
BssSI C'ACGA_G
Cuts at: 0 1045 1101
Size: 1045 56
BstEII G'GTnAC_C
Cuts at: 0 171 1101
Size: 171 930
BstYI r'GATC_y
Cuts at: 0 353 827 1096 1101
Size: 353 474 269 5
Fragments arranged by size:
474 353 269 5
Bsu36I CC'TnA_GG
Cuts at: 0 934 1101
Size: 934 167
BtgI C'CryG_G
Cuts at: 0 575 599 1101
Size: 575 24 502
Fragments arranged by size:
575 502 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 867 1101
Size: 867 234
EaeI y'GGCC_r
Cuts at: 0 542 917 1101
Size: 542 375 184
Fragments arranged by size:
542 375 184
EarI CTCTTCn'nnn_
Cuts at: 0 253 494 1101
Size: 253 241 607
Fragments arranged by size:
607 253 241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 380 388 1101
Size: 380 8 713
Fragments arranged by size:
713 380 8
EcoICRI GAG'CTC
Cuts at: 0 572 1101
Size: 572 529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 305 451 466 588 838 886 1101
Size: 91 214 146 15 122 250 48 215
Fragments arranged by size:
250 215 214 146 122 91 48 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 835 1101
Size: 835 266
EcoO109I rG'GnC_Cy
Cuts at: 0 387 968 1014 1101
Size: 387 581 46 87
Fragments arranged by size:
581 387 87 46
HaeII r_GCGC'y
Cuts at: 0 719 852 894 1101
Size: 719 133 42 207
Fragments arranged by size:
719 207 133 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 173 205 1081 1101
Size: 173 32 876 20
Fragments arranged by size:
876 173 32 20
HindIII A'AGCT_T
Cuts at: 0 422 1101
Size: 422 679
KasI G'GCGC_C
Cuts at: 0 715 848 1101
Size: 715 133 253
Fragments arranged by size:
715 253 133
MscI TGG'CCA
Cuts at: 0 544 919 1101
Size: 544 375 182
Fragments arranged by size:
544 375 182
MslI CAynn'nnrTG
Cuts at: 0 493 1101
Size: 493 608
MspA1I CmG'CkG
Cuts at: 0 444 684 915 1101
Size: 444 240 231 186
Fragments arranged by size:
444 240 231 186
NaeI GCC'GGC
Cuts at: 0 276 847 1101
Size: 276 571 254
Fragments arranged by size:
571 276 254
NarI GG'CG_CC
Cuts at: 0 716 849 1101
Size: 716 133 252
Fragments arranged by size:
716 252 133
NcoI C'CATG_G
Cuts at: 0 575 1101
Size: 575 526
NgoMIV G'CCGG_C
Cuts at: 0 274 845 1101
Size: 274 571 256
Fragments arranged by size:
571 274 256
NspI r_CATG'y
Cuts at: 0 657 1101
Size: 657 444
PciI A'CATG_T
Cuts at: 0 653 1101
Size: 653 448
PflMI CCAn_nnn'nTGG
Cuts at: 0 540 551 725 926 990 1101
Size: 540 11 174 201 64 111
Fragments arranged by size:
540 201 174 111 64 11
PfoI T'CCnGG_A
Cuts at: 0 394 1101
Size: 394 707
PpuMI rG'GwC_Cy
Cuts at: 0 1014 1101
Size: 1014 87
PshAI GACnn'nnGTC
Cuts at: 0 523 1101
Size: 523 578
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 640 672 1101
Size: 640 32 429
Fragments arranged by size:
640 429 32
PstI C_TGCA'G
Cuts at: 0 443 878 1101
Size: 443 435 223
Fragments arranged by size:
443 435 223
PvuII CAG'CTG
Cuts at: 0 444 684 915 1101
Size: 444 240 231 186
Fragments arranged by size:
444 240 231 186
SacI G_AGCT'C
Cuts at: 0 574 1101
Size: 574 527
ScaI AGT'ACT
Cuts at: 0 78 1101
Size: 78 1023
SexAI A'CCwGG_T
Cuts at: 0 724 1101
Size: 724 377
SfcI C'TryA_G
Cuts at: 0 72 439 874 1077 1092 1101
Size: 72 367 435 203 15 9
Fragments arranged by size:
435 367 203 72 15 9
SfoI GGC'GCC
Cuts at: 0 717 850 1101
Size: 717 133 251
Fragments arranged by size:
717 251 133
SmaI CCC'GGG
Cuts at: 0 63 1101
Size: 63 1038
SmlI C'TyrA_G
Cuts at: 0 786 1101
Size: 786 315
StuI AGG'CCT
Cuts at: 0 592 1101
Size: 592 509
StyI C'CwwG_G
Cuts at: 0 221 575 1101
Size: 221 354 526
Fragments arranged by size:
526 354 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 159 1101
Size: 159 942
TatI w'GTAC_w
Cuts at: 0 76 548 1101
Size: 76 472 553
Fragments arranged by size:
553 472 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 155 187 1101
Size: 155 32 914
Fragments arranged by size:
914 155 32
Tth111I GACn'n_nGTC
Cuts at: 0 726 1101
Size: 726 375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 582 1101
Size: 492 90 519
Fragments arranged by size:
519 492 90
XmaI C'CCGG_G
Cuts at: 0 61 1101
Size: 61 1040
Enzymes that do cut:
AccI AcuI AflIII AlwNI AvaI BanI BanII BbeI
BbsI BciVI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BssSI BstEII BstYI
Bsu36I BtgI BtgZI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HindIII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PpuMI PshAI PsrI PstI PvuII SacI ScaI
SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BclI BfrBI
BlpI BmtI Bpu10I BsaBI BsaWI BsaXI BsiEI BsiWI
BsmI BspEI BspHI BspMI BsrDI BsrGI BssHII BstAPI
BstBI BstXI BstZ17I BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI
SapI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI ZraI