Restriction map for
pF1KB3863
(Linear) (Six-base) MAPSORT of: KIBB3863.seq Check: 7225 from: 1 to: 2403
KIBB3863.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1881 2403
Size: 1881 522
AccI GT'mk_AC
Cuts at: 0 327 679 2403
Size: 327 352 1724
Fragments arranged by size:
1724 352 327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 1415 2360 2403
Size: 398 1017 945 43
Fragments arranged by size:
1017 945 398 43
AfeI AGC'GCT
Cuts at: 0 611 2101 2325 2403
Size: 611 1490 224 78
Fragments arranged by size:
1490 611 224 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1006 2403
Size: 1006 1397
ApaI G_GGCC'C
Cuts at: 0 70 2403
Size: 70 2333
AvrII C'CTAG_G
Cuts at: 0 1726 2403
Size: 1726 677
BanI G'GyrC_C
Cuts at: 0 150 642 1731 1834 2403
Size: 150 492 1089 103 569
Fragments arranged by size:
1089 569 492 150 103
BanII G_rGCy'C
Cuts at: 0 70 136 1021 1468 1551 2021 2403
Size: 70 66 885 447 83 470 382
Fragments arranged by size:
885 470 447 382 83 70 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 1421 2366 2403
Size: 1421 945 37
Fragments arranged by size:
1421 945 37
BbvCI CC'TCA_GC
Cuts at: 0 1186 2403
Size: 1186 1217
BglI GCCn_nnn'nGGC
Cuts at: 0 297 604 880 1808 2403
Size: 297 307 276 928 595
Fragments arranged by size:
928 595 307 297 276
BlpI GC'TnA_GC
Cuts at: 0 1557 1948 2403
Size: 1557 391 455
Fragments arranged by size:
1557 455 391
Bme1580I G_kGCm'C
Cuts at: 0 70 636 1734 2403
Size: 70 566 1098 669
Fragments arranged by size:
1098 669 566 70
BmrI ACTGGGnnnn_n'
Cuts at: 0 331 2403
Size: 331 2072
BmtI G_CTAG'C
Cuts at: 0 902 2403
Size: 902 1501
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1098 1485 2307 2403
Size: 1098 387 822 96
Fragments arranged by size:
1098 822 387 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 788 878 1425 1844 2319 2403
Size: 598 190 90 547 419 475 84
Fragments arranged by size:
598 547 475 419 190 90 84
Bpu10I CC'TnA_GC
Cuts at: 0 1186 2320 2403
Size: 1186 1134 83
Fragments arranged by size:
1186 1134 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 937 2056 2116 2403
Size: 736 201 1119 60 287
Fragments arranged by size:
1119 736 287 201 60
BsaAI yAC'GTr
Cuts at: 0 2029 2122 2403
Size: 2029 93 281
Fragments arranged by size:
2029 281 93
BsaWI w'CCGG_w
Cuts at: 0 330 2403
Size: 330 2073
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 764 783 794 813 2403
Size: 764 19 11 19 1590
Fragments arranged by size:
1590 764 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 396 674 791 1458 1495 1498 1510 1519
Size: 396 278 117 667 37 3 12 9
Cuts at: 1519 1915 1918 2343 2346 2403
Size: 396 3 425 3 57
Fragments arranged by size:
667 425 396 396 278 117 57 37
12 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 57 63 809 1699 1983 2403
Size: 57 6 746 890 284 420
Fragments arranged by size:
890 746 420 284 57 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1121 2279 2403
Size: 1121 1158 124
Fragments arranged by size:
1158 1121 124
BsiHKAI G_wGCw'C
Cuts at: 0 1021 1468 2403
Size: 1021 447 935
Fragments arranged by size:
1021 935 447
BsmI GAATG_Cn'
Cuts at: 0 51 2403
Size: 51 2352
BsmBI CGTCTCn'nnnn_
Cuts at: 0 657 2403
Size: 657 1746
Bsp1286I G_dGCh'C
Cuts at: 0 70 136 636 1021 1468 1551 1734 2021
Size: 70 66 500 385 447 83 183 287
Cuts at: 2021 2403
Size: 382
Fragments arranged by size:
500 447 385 382 287 183 83 70
66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1881 2403
Size: 1881 522
BsrBI CCG'CTC
Cuts at: 0 780 2403
Size: 780 1623
BsrDI GCAATG_nn'
Cuts at: 0 1053 1065 1316 2403
Size: 1053 12 251 1087
Fragments arranged by size:
1087 1053 251 12
BstBI TT'CG_AA
Cuts at: 0 99 2403
Size: 99 2304
BstZ17I GTA'TAC
Cuts at: 0 680 2403
Size: 680 1723
Bsu36I CC'TnA_GG
Cuts at: 0 923 2403
Size: 923 1480
BtgI C'CryG_G
Cuts at: 0 1178 2403
Size: 1178 1225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1537 2403
Size: 1537 866
BtsI GCAGTG_nn'
Cuts at: 0 627 1464 1782 1794 2315 2403
Size: 627 837 318 12 521 88
Fragments arranged by size:
837 627 521 318 88 12
EaeI y'GGCC_r
Cuts at: 0 289 1418 2403
Size: 289 1129 985
Fragments arranged by size:
1129 985 289
EarI CTCTTCn'nnn_
Cuts at: 0 85 660 1472 1508 1904 2039 2364 2403
Size: 85 575 812 36 396 135 325 39
Fragments arranged by size:
812 575 396 325 135 85 39 36
EcoICRI GAG'CTC
Cuts at: 0 1019 1466 2403
Size: 1019 447 937
Fragments arranged by size:
1019 937 447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 598 788 878 1415 1425 1844 2319
Size: 398 200 190 90 537 10 419 475
Cuts at: 2319 2360 2403
Size: 41 43
Fragments arranged by size:
537 475 419 398 200 190 90 43
41 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 38 247 523 1823 2403
Size: 38 209 276 1300 580
Fragments arranged by size:
1300 580 276 209 38
EcoO109I rG'GnC_Cy
Cuts at: 0 1381 1723 1801 2403
Size: 1381 342 78 602
Fragments arranged by size:
1381 602 342 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1051 2403
Size: 1051 1352
HaeII r_GCGC'y
Cuts at: 0 613 2103 2327 2403
Size: 613 1490 224 76
Fragments arranged by size:
1490 613 224 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 249 281 764 796 2335 2367 2380 2403
Size: 249 32 483 32 1539 32 13 23
Fragments arranged by size:
1539 483 249 32 32 32 23 13
HindIII A'AGCT_T
Cuts at: 0 1033 2403
Size: 1033 1370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1292 2403
Size: 1292 1111
MscI TGG'CCA
Cuts at: 0 291 1420 2403
Size: 291 1129 983
Fragments arranged by size:
1129 983 291
MslI CAynn'nnrTG
Cuts at: 0 1058 2403
Size: 1058 1345
MspA1I CmG'CkG
Cuts at: 0 234 1947 2231 2403
Size: 234 1713 284 172
Fragments arranged by size:
1713 284 234 172
NcoI C'CATG_G
Cuts at: 0 1178 2403
Size: 1178 1225
NheI G'CTAG_C
Cuts at: 0 898 2403
Size: 898 1505
NspI r_CATG'y
Cuts at: 0 1096 2403
Size: 1096 1307
PasI CC'CwG_GG
Cuts at: 0 34 1614 2403
Size: 34 1580 789
Fragments arranged by size:
1580 789 34
PsiI TTA'TAA
Cuts at: 0 349 2403
Size: 349 2054
PspOMI G'GGCC_C
Cuts at: 0 66 2403
Size: 66 2337
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1204 1236 2403
Size: 1204 32 1167
Fragments arranged by size:
1204 1167 32
PstI C_TGCA'G
Cuts at: 0 798 2403
Size: 798 1605
PvuII CAG'CTG
Cuts at: 0 234 1947 2403
Size: 234 1713 456
Fragments arranged by size:
1713 456 234
SacI G_AGCT'C
Cuts at: 0 1021 1468 2403
Size: 1021 447 935
Fragments arranged by size:
1021 935 447
SfcI C'TryA_G
Cuts at: 0 520 794 869 1324 1567 1883 2403
Size: 520 274 75 455 243 316 520
Fragments arranged by size:
520 520 455 316 274 243 75
SmlI C'TyrA_G
Cuts at: 0 751 916 2071 2095 2403
Size: 751 165 1155 24 308
Fragments arranged by size:
1155 751 308 165 24
SnaBI TAC'GTA
Cuts at: 0 2122 2403
Size: 2122 281
SphI G_CATG'C
Cuts at: 0 1096 2403
Size: 1096 1307
StyI C'CwwG_G
Cuts at: 0 1178 1726 2403
Size: 1178 548 677
Fragments arranged by size:
1178 677 548
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 740 2403
Size: 740 1663
TatI w'GTAC_w
Cuts at: 0 1280 1587 2403
Size: 1280 307 816
Fragments arranged by size:
1280 816 307
XmnI GAAnn'nnTTC
Cuts at: 0 1035 1577 2403
Size: 1035 542 826
Fragments arranged by size:
1035 826 542
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI ApaI AvrII BanI
BanII BbsI BbvCI BglI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BstBI BstZ17I Bsu36I BtgI BtgZI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HindIII MmeI MscI MslI MspA1I NcoI NheI NspI
PasI PsiI PspOMI PsrI PstI PvuII SacI SfcI
SmlI SnaBI SphI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII
BmgBI BsaI BsaBI BsaHI BsiEI BsiWI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspXI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SpeI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI