Restriction map for
pF1KB3860
(Linear) (Six-base) MAPSORT of: KIBB3860.seq Check: 1689 from: 1 to: 1044
KIBB3860.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 544 728 1044
Size: 544 184 316
Fragments arranged by size:
544 316 184
AccI GT'mk_AC
Cuts at: 0 149 1044
Size: 149 895
Acc65I G'GTAC_C
Cuts at: 0 116 1044
Size: 116 928
AfeI AGC'GCT
Cuts at: 0 922 1044
Size: 922 122
AflIII A'CryG_T
Cuts at: 0 38 572 692 1044
Size: 38 534 120 352
Fragments arranged by size:
534 352 120 38
AlwNI CAG_nnn'CTG
Cuts at: 0 214 250 559 1044
Size: 214 36 309 485
Fragments arranged by size:
485 309 214 36
ApaI G_GGCC'C
Cuts at: 0 140 1044
Size: 140 904
AscI GG'CGCG_CC
Cuts at: 0 95 1044
Size: 95 949
BamHI G'GATC_C
Cuts at: 0 481 1044
Size: 481 563
BanI G'GyrC_C
Cuts at: 0 116 523 991 1044
Size: 116 407 468 53
Fragments arranged by size:
468 407 116 53
BanII G_rGCy'C
Cuts at: 0 140 200 1044
Size: 140 60 844
Fragments arranged by size:
844 140 60
BbeI G_GCGC'C
Cuts at: 0 995 1044
Size: 995 49
BbvCI CC'TCA_GC
Cuts at: 0 121 1044
Size: 121 923
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 72 106 834 868 1044
Size: 72 34 728 34 176
Fragments arranged by size:
728 176 72 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 424 503 1044
Size: 424 79 541
Fragments arranged by size:
541 424 79
BlpI GC'TnA_GC
Cuts at: 0 1027 1044
Size: 1027 17
Bme1580I G_kGCm'C
Cuts at: 0 140 1044
Size: 140 904
BmgBI CAC'GTC
Cuts at: 0 605 677 1044
Size: 605 72 367
Fragments arranged by size:
605 367 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1044
Size: 79 965
Bpu10I CC'TnA_GC
Cuts at: 0 121 616 1044
Size: 121 495 428
Fragments arranged by size:
495 428 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1044
Size: 928 116
BsaAI yAC'GTr
Cuts at: 0 132 693 1044
Size: 132 561 351
Fragments arranged by size:
561 351 132
BsaHI Gr'CG_yC
Cuts at: 0 161 344 443 992 1044
Size: 161 183 99 549 52
Fragments arranged by size:
549 183 161 99 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 1044
Size: 187 30 827
Fragments arranged by size:
827 187 30
BseYI C'CCAG_C
Cuts at: 0 228 1044
Size: 228 816
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1044
Size: 88 956
BsiEI CG_ry'CG
Cuts at: 0 53 320 567 861 1044
Size: 53 267 247 294 183
Fragments arranged by size:
294 267 247 183 53
BsmI GAATG_Cn'
Cuts at: 0 891 1044
Size: 891 153
Bsp1286I G_dGCh'C
Cuts at: 0 140 200 1044
Size: 140 60 844
Fragments arranged by size:
844 140 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 544 728 743 878 1044
Size: 544 184 15 135 166
Fragments arranged by size:
544 184 166 135 15
BsrBI CCG'CTC
Cuts at: 0 406 863 1044
Size: 406 457 181
Fragments arranged by size:
457 406 181
BsrFI r'CCGG_y
Cuts at: 0 391 566 702 811 1044
Size: 391 175 136 109 233
Fragments arranged by size:
391 233 175 136 109
BssHII G'CGCG_C
Cuts at: 0 95 510 532 534 833 873 875 1044
Size: 95 415 22 2 299 40 2 169
Fragments arranged by size:
415 299 169 95 40 22 2 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 250 735 923 1044
Size: 250 485 188 121
Fragments arranged by size:
485 250 188 121
BstYI r'GATC_y
Cuts at: 0 481 1044
Size: 481 563
BtgI C'CryG_G
Cuts at: 0 707 1004 1044
Size: 707 297 40
Fragments arranged by size:
707 297 40
BtsI GCAGTG_nn'
Cuts at: 0 100 837 1044
Size: 100 737 207
Fragments arranged by size:
737 207 100
DrdI GACnn_nn'nnGTC
Cuts at: 0 319 1044
Size: 319 725
EaeI y'GGCC_r
Cuts at: 0 50 281 317 564 704 710 1044
Size: 50 231 36 247 140 6 334
Fragments arranged by size:
334 247 231 140 50 36 6
EagI C'GGCC_G
Cuts at: 0 50 317 564 1044
Size: 50 267 247 480
Fragments arranged by size:
480 267 247 50
EarI CTCTTCn'nnn_
Cuts at: 0 51 925 1044
Size: 51 874 119
Fragments arranged by size:
874 119 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 355 1044
Size: 355 689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1044
Size: 79 965
EcoO109I rG'GnC_Cy
Cuts at: 0 8 136 137 1000 1044
Size: 8 128 1 863 44
Fragments arranged by size:
863 128 44 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 951 1044
Size: 951 93
HaeII r_GCGC'y
Cuts at: 0 68 369 669 924 995 1044
Size: 68 301 300 255 71 49
Fragments arranged by size:
301 300 255 71 68 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 352 384 1044
Size: 28 32 292 32 660
Fragments arranged by size:
660 292 32 32 28
KasI G'GCGC_C
Cuts at: 0 991 1044
Size: 991 53
KpnI G_GTAC'C
Cuts at: 0 120 1044
Size: 120 924
MluI A'CGCG_T
Cuts at: 0 38 572 1044
Size: 38 534 472
Fragments arranged by size:
534 472 38
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 1044
Size: 890 154
MscI TGG'CCA
Cuts at: 0 283 712 1044
Size: 283 429 332
Fragments arranged by size:
429 332 283
MslI CAynn'nnrTG
Cuts at: 0 712 1044
Size: 712 332
MspA1I CmG'CkG
Cuts at: 0 214 289 657 1006 1044
Size: 214 75 368 349 38
Fragments arranged by size:
368 349 214 75 38
NaeI GCC'GGC
Cuts at: 0 393 568 704 1044
Size: 393 175 136 340
Fragments arranged by size:
393 340 175 136
NarI GG'CG_CC
Cuts at: 0 992 1044
Size: 992 52
NcoI C'CATG_G
Cuts at: 0 707 1044
Size: 707 337
NgoMIV G'CCGG_C
Cuts at: 0 391 566 702 1044
Size: 391 175 136 342
Fragments arranged by size:
391 342 175 136
NspI r_CATG'y
Cuts at: 0 801 885 1044
Size: 801 84 159
Fragments arranged by size:
801 159 84
PpuMI rG'GwC_Cy
Cuts at: 0 8 1044
Size: 8 1036
PspOMI G'GGCC_C
Cuts at: 0 136 1044
Size: 136 908
PstI C_TGCA'G
Cuts at: 0 246 791 1044
Size: 246 545 253
Fragments arranged by size:
545 253 246
PvuII CAG'CTG
Cuts at: 0 214 657 1044
Size: 214 443 387
Fragments arranged by size:
443 387 214
SacII CC_GC'GG
Cuts at: 0 1007 1044
Size: 1007 37
SapI GCTCTTCn'nnn_
Cuts at: 0 925 1044
Size: 925 119
SfcI C'TryA_G
Cuts at: 0 242 787 1044
Size: 242 545 257
Fragments arranged by size:
545 257 242
SfoI GGC'GCC
Cuts at: 0 993 1044
Size: 993 51
SgrAI Cr'CCGG_yG
Cuts at: 0 811 1044
Size: 811 233
SmlI C'TyrA_G
Cuts at: 0 943 1044
Size: 943 101
StuI AGG'CCT
Cuts at: 0 850 1044
Size: 850 194
StyI C'CwwG_G
Cuts at: 0 707 1044
Size: 707 337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 705 1044
Size: 12 693 339
Fragments arranged by size:
693 339 12
XmnI GAAnn'nnTTC
Cuts at: 0 823 934 1044
Size: 823 111 110
Fragments arranged by size:
823 111 110
Enzymes that do cut:
AarI AccI Acc65I AfeI AflIII AlwNI ApaI AscI
BamHI BanI BanII BbeI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseYI BsgI BsiEI BsmI Bsp1286I BspMI BsrBI BsrFI
BssHII BstAPI BstYI BtgI BtsI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PpuMI PspOMI PstI PvuII SacII
SapI SfcI SfoI SgrAI SmlI StuI StyI XcmI
XmnI
Enzymes that do not cut:
AatII AclI AcuI AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BbsI BciVI BclI BfrBI BglII BmrI BmtI BplI
BsaI BsaBI BsaWI BseRI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI
ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI ZraI