Restriction map for
pF1KB3849
(Linear) (Six-base) MAPSORT of: KIBB3849.seq Check: 5010 from: 1 to: 2769
KIBB3849.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2713 2769
Size: 2713 56
AatII G_ACGT'C
Cuts at: 0 1322 2769
Size: 1322 1447
AccI GT'mk_AC
Cuts at: 0 2377 2657 2769
Size: 2377 280 112
Fragments arranged by size:
2377 280 112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1821 2769
Size: 1821 948
AflIII A'CryG_T
Cuts at: 0 734 1499 2769
Size: 734 765 1270
Fragments arranged by size:
1270 765 734
AleI CACnn'nnGTG
Cuts at: 0 778 2769
Size: 778 1991
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 148 180 2186 2218 2769
Size: 148 32 2006 32 551
Fragments arranged by size:
2006 551 148 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 799 1911 2769
Size: 799 1112 858
Fragments arranged by size:
1112 858 799
ApoI r'AATT_y
Cuts at: 0 313 394 405 870 1185 2769
Size: 313 81 11 465 315 1584
Fragments arranged by size:
1584 465 315 313 81 11
AseI AT'TA_AT
Cuts at: 0 377 538 992 2769
Size: 377 161 454 1777
Fragments arranged by size:
1777 454 377 161
AvaI C'yCGr_G
Cuts at: 0 112 1828 2769
Size: 112 1716 941
Fragments arranged by size:
1716 941 112
BamHI G'GATC_C
Cuts at: 0 686 2769
Size: 686 2083
BanI G'GyrC_C
Cuts at: 0 10 670 772 1068 1719 2446 2479 2578
Size: 10 660 102 296 651 727 33 99
Cuts at: 2578 2584 2769
Size: 6 185
Fragments arranged by size:
727 660 651 296 185 102 99 33
10 6
BanII G_rGCy'C
Cuts at: 0 133 683 1603 1739 1829 1835 2563 2769
Size: 133 550 920 136 90 6 728 206
Fragments arranged by size:
920 728 550 206 136 133 90 6
BbeI G_GCGC'C
Cuts at: 0 14 2769
Size: 14 2755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1684 1718 2769
Size: 1684 34 1051
Fragments arranged by size:
1684 1051 34
BclI T'GATC_A
Cuts at: 0 1091 2769
Size: 1091 1678
BlpI GC'TnA_GC
Cuts at: 0 1612 2045 2769
Size: 1612 433 724
Fragments arranged by size:
1612 724 433
Bme1580I G_kGCm'C
Cuts at: 0 2639 2769
Size: 2639 130
BmgBI CAC'GTC
Cuts at: 0 735 2769
Size: 735 2034
BmrI ACTGGGnnnn_n'
Cuts at: 0 2610 2769
Size: 2610 159
BmtI G_CTAG'C
Cuts at: 0 1823 2769
Size: 1823 946
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1272 2127 2178 2769
Size: 607 665 855 51 591
Fragments arranged by size:
855 665 607 591 51
Bpu10I CC'TnA_GC
Cuts at: 0 664 2769
Size: 664 2105
BsaI GGTCTCn'nnnn_
Cuts at: 0 913 2182 2433 2769
Size: 913 1269 251 336
Fragments arranged by size:
1269 913 336 251
BsaAI yAC'GTr
Cuts at: 0 354 1500 1809 2634 2769
Size: 354 1146 309 825 135
Fragments arranged by size:
1146 825 354 309 135
BsaHI Gr'CG_yC
Cuts at: 0 11 701 1319 1351 2533 2769
Size: 11 690 618 32 1182 236
Fragments arranged by size:
1182 690 618 236 32 11
BsaWI w'CCGG_w
Cuts at: 0 683 1335 2769
Size: 683 652 1434
Fragments arranged by size:
1434 683 652
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 942 972 2769
Size: 942 30 1797
Fragments arranged by size:
1797 942 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 1275 2130 2769
Size: 292 983 855 639
Fragments arranged by size:
983 855 639 292
BseYI C'CCAG_C
Cuts at: 0 21 1686 2718 2769
Size: 21 1665 1032 51
Fragments arranged by size:
1665 1032 51 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 1951 2769
Size: 419 1532 818
Fragments arranged by size:
1532 818 419
BsiEI CG_ry'CG
Cuts at: 0 1650 2769
Size: 1650 1119
BsiHKAI G_wGCw'C
Cuts at: 0 50 309 683 2093 2563 2769
Size: 50 259 374 1410 470 206
Fragments arranged by size:
1410 470 374 259 206 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 725 1310 2769
Size: 725 585 1459
Fragments arranged by size:
1459 725 585
Bsp1286I G_dGCh'C
Cuts at: 0 50 133 309 683 1603 1739 1829 1835
Size: 50 83 176 374 920 136 90 6
Cuts at: 1835 2093 2563 2639 2769
Size: 258 470 76 130
Fragments arranged by size:
920 470 374 258 176 136 130 90
83 76 50 6
BspEI T'CCGG_A
Cuts at: 0 683 2769
Size: 683 2086
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2713 2769
Size: 2713 56
BsrBI CCG'CTC
Cuts at: 0 8 242 881 2769
Size: 8 234 639 1888
Fragments arranged by size:
1888 639 234 8
BsrDI GCAATG_nn'
Cuts at: 0 2679 2736 2769
Size: 2679 57 33
Fragments arranged by size:
2679 57 33
BsrFI r'CCGG_y
Cuts at: 0 2476 2581 2769
Size: 2476 105 188
Fragments arranged by size:
2476 188 105
BsrGI T'GTAC_A
Cuts at: 0 737 979 2769
Size: 737 242 1790
Fragments arranged by size:
1790 737 242
BssHII G'CGCG_C
Cuts at: 0 1511 1539 1725 2769
Size: 1511 28 186 1044
Fragments arranged by size:
1511 1044 186 28
BstAPI GCAn_nnn'nTGC
Cuts at: 0 475 1998 2634 2769
Size: 475 1523 636 135
Fragments arranged by size:
1523 636 475 135
BstBI TT'CG_AA
Cuts at: 0 1571 2769
Size: 1571 1198
BstEII G'GTnAC_C
Cuts at: 0 693 1749 2769
Size: 693 1056 1020
Fragments arranged by size:
1056 1020 693
BstXI CCAn_nnnn'nTGG
Cuts at: 0 159 1114 1761 2529 2769
Size: 159 955 647 768 240
Fragments arranged by size:
955 768 647 240 159
BstYI r'GATC_y
Cuts at: 0 95 686 2769
Size: 95 591 2083
Fragments arranged by size:
2083 591 95
BtgI C'CryG_G
Cuts at: 0 152 1666 1766 2588 2769
Size: 152 1514 100 822 181
Fragments arranged by size:
1514 822 181 152 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1383 2483 2769
Size: 1383 1100 286
Fragments arranged by size:
1383 1100 286
EaeI y'GGCC_r
Cuts at: 0 1440 1463 1763 2102 2114 2769
Size: 1440 23 300 339 12 655
Fragments arranged by size:
1440 655 339 300 23 12
EarI CTCTTCn'nnn_
Cuts at: 0 61 911 1179 1714 1840 2098 2769
Size: 61 850 268 535 126 258 671
Fragments arranged by size:
850 671 535 268 258 126 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1941 2526 2769
Size: 1941 585 243
Fragments arranged by size:
1941 585 243
EcoICRI GAG'CTC
Cuts at: 0 681 2561 2769
Size: 681 1880 208
Fragments arranged by size:
1880 681 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1272 1821 2127 2178 2769
Size: 607 665 549 306 51 591
Fragments arranged by size:
665 607 591 549 306 51
EcoO109I rG'GnC_Cy
Cuts at: 0 1391 1690 2345 2715 2769
Size: 1391 299 655 370 54
Fragments arranged by size:
1391 655 370 299 54
EcoRI G'AATT_C
Cuts at: 0 870 2769
Size: 870 1899
EcoRV GAT'ATC
Cuts at: 0 2010 2769
Size: 2010 759
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 237 2341 2769
Size: 237 2104 428
Fragments arranged by size:
2104 428 237
FspI TGC'GCA
Cuts at: 0 1564 1852 1867 2769
Size: 1564 288 15 902
Fragments arranged by size:
1564 902 288 15
FspAI rTGC'GCAy
Cuts at: 0 1867 2769
Size: 1867 902
HaeII r_GCGC'y
Cuts at: 0 14 85 1911 2603 2769
Size: 14 71 1826 692 166
Fragments arranged by size:
1826 692 166 71 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 606 638 1585 1617 1762 1794 2370 2402
Size: 606 32 947 32 145 32 576 32
Cuts at: 2402 2545 2577 2769
Size: 143 32 192
Fragments arranged by size:
947 606 576 192 145 143 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1323 2378 2769
Size: 1323 1055 391
Fragments arranged by size:
1323 1055 391
KasI G'GCGC_C
Cuts at: 0 10 2769
Size: 10 2759
MfeI C'AATT_G
Cuts at: 0 2302 2769
Size: 2302 467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2193 2769
Size: 2193 576
MscI TGG'CCA
Cuts at: 0 1465 2769
Size: 1465 1304
MslI CAynn'nnrTG
Cuts at: 0 356 778 2509 2769
Size: 356 422 1731 260
Fragments arranged by size:
1731 422 356 260
MspA1I CmG'CkG
Cuts at: 0 1560 1668 2453 2695 2769
Size: 1560 108 785 242 74
Fragments arranged by size:
1560 785 242 108 74
NaeI GCC'GGC
Cuts at: 0 2583 2769
Size: 2583 186
NarI GG'CG_CC
Cuts at: 0 11 2769
Size: 11 2758
NcoI C'CATG_G
Cuts at: 0 2588 2769
Size: 2588 181
NgoMIV G'CCGG_C
Cuts at: 0 2581 2769
Size: 2581 188
NheI G'CTAG_C
Cuts at: 0 1819 2769
Size: 1819 950
PasI CC'CwG_GG
Cuts at: 0 2568 2769
Size: 2568 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 2725 2769
Size: 2725 44
PfoI T'CCnGG_A
Cuts at: 0 173 2769
Size: 173 2596
PmlI CAC'GTG
Cuts at: 0 354 1809 2634 2769
Size: 354 1455 825 135
Fragments arranged by size:
1455 825 354 135
PpuMI rG'GwC_Cy
Cuts at: 0 1690 2769
Size: 1690 1079
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1565 1597 2769
Size: 1565 32 1172
Fragments arranged by size:
1565 1172 32
PstI C_TGCA'G
Cuts at: 0 503 1734 1817 2054 2630 2769
Size: 503 1231 83 237 576 139
Fragments arranged by size:
1231 576 503 237 139 83
PvuII CAG'CTG
Cuts at: 0 1560 2695 2769
Size: 1560 1135 74
Fragments arranged by size:
1560 1135 74
SacI G_AGCT'C
Cuts at: 0 683 2563 2769
Size: 683 1880 206
Fragments arranged by size:
1880 683 206
SacII CC_GC'GG
Cuts at: 0 1669 2769
Size: 1669 1100
SalI G'TCGA_C
Cuts at: 0 2376 2769
Size: 2376 393
SanDI GG'GwC_CC
Cuts at: 0 1690 2769
Size: 1690 1079
SapI GCTCTTCn'nnn_
Cuts at: 0 1714 1840 2098 2769
Size: 1714 126 258 671
Fragments arranged by size:
1714 671 258 126
SexAI A'CCwGG_T
Cuts at: 0 1745 2172 2769
Size: 1745 427 597
Fragments arranged by size:
1745 597 427
SfcI C'TryA_G
Cuts at: 0 102 499 843 1582 1730 1813 2050 2214
Size: 102 397 344 739 148 83 237 164
Cuts at: 2214 2626 2769
Size: 412 143
Fragments arranged by size:
739 412 397 344 237 164 148 143
102 83
SfoI GGC'GCC
Cuts at: 0 12 2769
Size: 12 2757
SmaI CCC'GGG
Cuts at: 0 1830 2769
Size: 1830 939
SrfI GCCC'GGGC
Cuts at: 0 1830 2769
Size: 1830 939
SspI AAT'ATT
Cuts at: 0 931 996 2769
Size: 931 65 1773
Fragments arranged by size:
1773 931 65
StyI C'CwwG_G
Cuts at: 0 125 641 923 1754 1880 2072 2588 2769
Size: 125 516 282 831 126 192 516 181
Fragments arranged by size:
831 516 516 282 192 181 126 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1235 1664 2769
Size: 1235 429 1105
Fragments arranged by size:
1235 1105 429
TatI w'GTAC_w
Cuts at: 0 737 979 1238 2769
Size: 737 242 259 1531
Fragments arranged by size:
1531 737 259 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 942 974 1336 1368 2769
Size: 942 32 362 32 1401
Fragments arranged by size:
1401 942 362 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1968 2769
Size: 1968 801
XmaI C'CCGG_G
Cuts at: 0 1828 2769
Size: 1828 941
XmnI GAAnn'nnTTC
Cuts at: 0 1052 2769
Size: 1052 1717
ZraI GAC'GTC
Cuts at: 0 1320 2769
Size: 1320 1449
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AloI AlwNI
ApoI AseI AvaI BamHI BanI BanII BbeI BcgI
BclI BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI PasI PflMI PfoI PmlI PpuMI
PsrI PstI PvuII SacI SacII SalI SanDI SapI
SexAI SfcI SfoI SmaI SrfI SspI StyI TaqII
TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaI
ApaLI AscI AsiSI AvrII BaeI BbsI BbvCI BciVI
BfrBI BglI BglII BplI BpuEI BsaBI BsiWI BsmI
BspHI BssSI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoNI FseI HindIII HpaI KpnI
MluI NdeI NotI NruI NsiI NspI PacI PciI
PmeI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI StuI SwaI Tth111I XbaI XhoI