Restriction map for
pF1KB3824
(Linear) (Six-base) MAPSORT of: KIBB3824.seq Check: 4366 from: 1 to: 3108
KIBB3824.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1407 1933 2113 3108
Size: 1407 526 180 995
Fragments arranged by size:
1407 995 526 180
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1678 1775 2445 3108
Size: 584 1094 97 670 663
Fragments arranged by size:
1094 670 663 584 97
AflIII A'CryG_T
Cuts at: 0 191 1040 3108
Size: 191 849 2068
Fragments arranged by size:
2068 849 191
AgeI A'CCGG_T
Cuts at: 0 1241 3108
Size: 1241 1867
AhdI GACnn_n'nnGTC
Cuts at: 0 800 3108
Size: 800 2308
AleI CACnn'nnGTG
Cuts at: 0 1673 2462 2792 3108
Size: 1673 789 330 316
Fragments arranged by size:
1673 789 330 316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 864 3108
Size: 864 2244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2071 2103 3108
Size: 2071 32 1005
Fragments arranged by size:
2071 1005 32
AlwNI CAG_nnn'CTG
Cuts at: 0 149 626 3108
Size: 149 477 2482
Fragments arranged by size:
2482 477 149
ApaLI G'TGCA_C
Cuts at: 0 2100 3108
Size: 2100 1008
AseI AT'TA_AT
Cuts at: 0 1448 3108
Size: 1448 1660
AvaI C'yCGr_G
Cuts at: 0 95 153 3108
Size: 95 58 2955
Fragments arranged by size:
2955 95 58
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2298 2331 3108
Size: 2298 33 777
Fragments arranged by size:
2298 777 33
BamHI G'GATC_C
Cuts at: 0 916 3108
Size: 916 2192
BanI G'GyrC_C
Cuts at: 0 41 91 226 1874 1932 3108
Size: 41 50 135 1648 58 1176
Fragments arranged by size:
1648 1176 135 58 50 41
BanII G_rGCy'C
Cuts at: 0 143 1709 2018 2585 3108
Size: 143 1566 309 567 523
Fragments arranged by size:
1566 567 523 309 143
BbsI GAAGACnn'nnnn_
Cuts at: 0 1259 2435 2668 3108
Size: 1259 1176 233 440
Fragments arranged by size:
1259 1176 440 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 571 605 3108
Size: 571 34 2503
Fragments arranged by size:
2503 571 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1193 2385 2891 3108
Size: 1193 1192 506 217
Fragments arranged by size:
1193 1192 506 217
BclI T'GATC_A
Cuts at: 0 1919 3108
Size: 1919 1189
BglI GCCn_nnn'nGGC
Cuts at: 0 1082 2531 3108
Size: 1082 1449 577
Fragments arranged by size:
1449 1082 577
BglII A'GATC_T
Cuts at: 0 1583 1721 3108
Size: 1583 138 1387
Fragments arranged by size:
1583 1387 138
Bme1580I G_kGCm'C
Cuts at: 0 44 1145 1819 1917 2104 2146 3108
Size: 44 1101 674 98 187 42 962
Fragments arranged by size:
1101 962 674 187 98 44 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 1101 1156 3108
Size: 1101 55 1952
Fragments arranged by size:
1952 1101 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 897 3108
Size: 897 2211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2066 2710 2743 3108
Size: 2066 644 33 365
Fragments arranged by size:
2066 644 365 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 2415 3108
Size: 809 1606 693
Fragments arranged by size:
1606 809 693
BsaI GGTCTCn'nnnn_
Cuts at: 0 57 1232 3108
Size: 57 1175 1876
Fragments arranged by size:
1876 1175 57
BsaAI yAC'GTr
Cuts at: 0 194 1140 3108
Size: 194 946 1968
Fragments arranged by size:
1968 946 194
BsaBI GATnn'nnATC
Cuts at: 0 1838 3108
Size: 1838 1270
BsaHI Gr'CG_yC
Cuts at: 0 173 3108
Size: 173 2935
BsaWI w'CCGG_w
Cuts at: 0 1241 3108
Size: 1241 1867
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2071 2101 3108
Size: 2071 30 1007
Fragments arranged by size:
2071 1007 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 584 1870 2063 2500 2690 3108
Size: 151 433 1286 193 437 190 418
Fragments arranged by size:
1286 437 433 418 193 190 151
BseYI C'CCAG_C
Cuts at: 0 60 619 1795 3108
Size: 60 559 1176 1313
Fragments arranged by size:
1313 1176 559 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1245 1308 1407 1518 1635 1794 2006
Size: 187 1058 63 99 111 117 159 212
Cuts at: 2006 2023 2493 2770 3108
Size: 17 470 277 338
Fragments arranged by size:
1058 470 338 277 212 187 159 117
111 99 63 17
BsiEI CG_ry'CG
Cuts at: 0 255 1307 2552 3108
Size: 255 1052 1245 556
Fragments arranged by size:
1245 1052 556 255
BsiHKAI G_wGCw'C
Cuts at: 0 1709 2018 2104 3108
Size: 1709 309 86 1004
Fragments arranged by size:
1709 1004 309 86
BsmI GAATG_Cn'
Cuts at: 0 781 1888 2866 3017 3108
Size: 781 1107 978 151 91
Fragments arranged by size:
1107 978 781 151 91
Bsp1286I G_dGCh'C
Cuts at: 0 44 143 1145 1709 1819 1917 2018 2104
Size: 44 99 1002 564 110 98 101 86
Cuts at: 2104 2146 2585 3108
Size: 42 439 523
Fragments arranged by size:
1002 564 523 439 110 101 99 98
86 44 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 256 448 1330 1407 1933 2113 3108
Size: 256 192 882 77 526 180 995
Fragments arranged by size:
995 882 526 256 192 180 77
BsrBI CCG'CTC
Cuts at: 0 285 2490 3108
Size: 285 2205 618
Fragments arranged by size:
2205 618 285
BsrDI GCAATG_nn'
Cuts at: 0 381 2816 3108
Size: 381 2435 292
Fragments arranged by size:
2435 381 292
BsrFI r'CCGG_y
Cuts at: 0 31 1241 3108
Size: 31 1210 1867
Fragments arranged by size:
1867 1210 31
BsrGI T'GTAC_A
Cuts at: 0 2200 3108
Size: 2200 908
BssHII G'CGCG_C
Cuts at: 0 82 3108
Size: 82 3026
BssSI C'ACGA_G
Cuts at: 0 938 3108
Size: 938 2170
BstAPI GCAn_nnn'nTGC
Cuts at: 0 662 1268 2516 3108
Size: 662 606 1248 592
Fragments arranged by size:
1248 662 606 592
BstBI TT'CG_AA
Cuts at: 0 435 1047 3108
Size: 435 612 2061
Fragments arranged by size:
2061 612 435
BstEII G'GTnAC_C
Cuts at: 0 2752 3108
Size: 2752 356
BstXI CCAn_nnnn'nTGG
Cuts at: 0 358 2044 2464 2592 3108
Size: 358 1686 420 128 516
Fragments arranged by size:
1686 516 420 358 128
BstYI r'GATC_y
Cuts at: 0 916 1173 1583 1721 3108
Size: 916 257 410 138 1387
Fragments arranged by size:
1387 916 410 257 138
Bsu36I CC'TnA_GG
Cuts at: 0 702 3108
Size: 702 2406
BtgI C'CryG_G
Cuts at: 0 1233 1829 3108
Size: 1233 596 1279
Fragments arranged by size:
1279 1233 596
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 959 3108
Size: 959 2149
BtsI GCAGTG_nn'
Cuts at: 0 2514 2902 3108
Size: 2514 388 206
Fragments arranged by size:
2514 388 206
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1634 1669 2429 2464 3108
Size: 1634 35 760 35 644
Fragments arranged by size:
1634 760 644 35 35
DraI TTT'AAA
Cuts at: 0 366 491 3108
Size: 366 125 2617
Fragments arranged by size:
2617 366 125
EaeI y'GGCC_r
Cuts at: 0 29 3108
Size: 29 3079
EarI CTCTTCn'nnn_
Cuts at: 0 500 3108
Size: 500 2608
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 265 3108
Size: 265 2843
EcoICRI GAG'CTC
Cuts at: 0 1707 2016 3108
Size: 1707 309 1092
Fragments arranged by size:
1707 1092 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1678 1775 2066 2445 2710 2743 3108
Size: 584 1094 97 291 379 265 33 365
Fragments arranged by size:
1094 584 379 365 291 265 97 33
EcoO109I rG'GnC_Cy
Cuts at: 0 2496 3108
Size: 2496 612
EcoRV GAT'ATC
Cuts at: 0 2727 3108
Size: 2727 381
FspI TGC'GCA
Cuts at: 0 655 3108
Size: 655 2453
HaeII r_GCGC'y
Cuts at: 0 103 3108
Size: 103 3005
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 786 818 901 933 1610 1642 2655 2687
Size: 786 32 83 32 677 32 1013 32
Cuts at: 2687 3108
Size: 421
Fragments arranged by size:
1013 786 677 421 83 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1281 2685 3108
Size: 1281 1404 423
Fragments arranged by size:
1404 1281 423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1459 3108
Size: 1459 1649
MslI CAynn'nnrTG
Cuts at: 0 688 1401 1673 1850 2462 2590 2792 3108
Size: 688 713 272 177 612 128 202 316
Fragments arranged by size:
713 688 612 316 272 202 177 128
MspA1I CmG'CkG
Cuts at: 0 533 626 1201 1795 2114 2151 2290 2321
Size: 533 93 575 594 319 37 139 31
Cuts at: 2321 2507 2558 3108
Size: 186 51 550
Fragments arranged by size:
594 575 550 533 319 186 139 93
51 37 31
NaeI GCC'GGC
Cuts at: 0 33 3108
Size: 33 3075
NgoMIV G'CCGG_C
Cuts at: 0 31 3108
Size: 31 3077
NspI r_CATG'y
Cuts at: 0 1044 1406 3063 3108
Size: 1044 362 1657 45
Fragments arranged by size:
1657 1044 362 45
PciI A'CATG_T
Cuts at: 0 1040 3108
Size: 1040 2068
PfoI T'CCnGG_A
Cuts at: 0 2296 3108
Size: 2296 812
PmlI CAC'GTG
Cuts at: 0 194 3108
Size: 194 2914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2071 2103 3108
Size: 2071 32 1005
Fragments arranged by size:
2071 1005 32
PpuMI rG'GwC_Cy
Cuts at: 0 2496 3108
Size: 2496 612
PshAI GACnn'nnGTC
Cuts at: 0 1058 1682 3108
Size: 1058 624 1426
Fragments arranged by size:
1426 1058 624
PsiI TTA'TAA
Cuts at: 0 2860 3108
Size: 2860 248
PstI C_TGCA'G
Cuts at: 0 445 766 843 2531 3108
Size: 445 321 77 1688 577
Fragments arranged by size:
1688 577 445 321 77
PvuII CAG'CTG
Cuts at: 0 1795 2290 2321 2507 2558 3108
Size: 1795 495 31 186 51 550
Fragments arranged by size:
1795 550 495 186 51 31
SacI G_AGCT'C
Cuts at: 0 1709 2018 3108
Size: 1709 309 1090
Fragments arranged by size:
1709 1090 309
SbfI CC_TGCA'GG
Cuts at: 0 2531 3108
Size: 2531 577
ScaI AGT'ACT
Cuts at: 0 2022 3108
Size: 2022 1086
SfcI C'TryA_G
Cuts at: 0 441 762 839 2527 3054 3079 3108
Size: 441 321 77 1688 527 25 29
Fragments arranged by size:
1688 527 441 321 77 29 25
SmaI CCC'GGG
Cuts at: 0 97 155 3108
Size: 97 58 2953
Fragments arranged by size:
2953 97 58
SmlI C'TyrA_G
Cuts at: 0 824 2394 3108
Size: 824 1570 714
Fragments arranged by size:
1570 824 714
SpeI A'CTAG_T
Cuts at: 0 687 3108
Size: 687 2421
SphI G_CATG'C
Cuts at: 0 3063 3108
Size: 3063 45
SspI AAT'ATT
Cuts at: 0 1027 3108
Size: 1027 2081
StuI AGG'CCT
Cuts at: 0 1783 2473 3108
Size: 1783 690 635
Fragments arranged by size:
1783 690 635
StyI C'CwwG_G
Cuts at: 0 524 1077 1600 1730 3108
Size: 524 553 523 130 1378
Fragments arranged by size:
1378 553 524 523 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1818 1862 2191 2527 2804 3108
Size: 1818 44 329 336 277 304
Fragments arranged by size:
1818 336 329 304 277 44
TatI w'GTAC_w
Cuts at: 0 2 853 2020 2092 2200 3108
Size: 2 851 1167 72 108 908
Fragments arranged by size:
1167 908 851 108 72 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1377 1409 3108
Size: 1377 32 1699
Fragments arranged by size:
1699 1377 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2909 2991 3108
Size: 2909 82 117
Fragments arranged by size:
2909 117 82
XmaI C'CCGG_G
Cuts at: 0 95 153 3108
Size: 95 58 2955
Fragments arranged by size:
2955 95 58
Enzymes that do cut:
AarI AcuI AflIII AgeI AhdI AleI AlfI AloI
AlwNI ApaLI AseI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRV FspI HaeII Hin4I HincII MmeI MslI MspA1I
NaeI NgoMIV NspI PciI PfoI PmlI PpiI PpuMI
PshAI PsiI PstI PvuII SacI SbfI ScaI SfcI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII ApaI ApoI
AscI AsiSI AvrII BbeI BbvCI BfrBI BlpI BmgBI
BmtI Bpu10I BsiWI BsmBI BspEI BspHI BstZ17I ClaI
DraIII DrdI EagI EcoNI EcoRI FalI FseI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmeI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI
SnaBI SrfI SwaI Tth111I XbaI XhoI XmnI ZraI