(Linear) (Six-base) MAPSORT of: KIBB3778.seq  Check: 1502  from: 1  to: 729



KIBB3778.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     722     729 
   Size:        722       7
 
AhdI GACnn_n'nnGTC

Cuts at:      0     539     729 
   Size:        539     190
 
ApaI G_GGCC'C

Cuts at:      0      94     729 
   Size:         94     635
 
ApoI r'AATT_y

Cuts at:      0     107     156     729 
   Size:        107      49     573

  Fragments arranged by size: 

                 573     107      49
 
AvaI C'yCGr_G

Cuts at:      0     122     676     729 
   Size:        122     554      53

  Fragments arranged by size: 

                 554     122      53
 
BanI G'GyrC_C

Cuts at:      0     366     729 
   Size:        366     363
 
BanII G_rGCy'C

Cuts at:      0      94     729 
   Size:         94     635
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     191     205     729 
   Size:        191      14     524

  Fragments arranged by size: 

                 524     191      14
 
Bme1580I G_kGCm'C

Cuts at:      0      94     729 
   Size:         94     635
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     334     729 
   Size:        334     395
 
BsaHI Gr'CG_yC

Cuts at:      0     535     729 
   Size:        535     194
 
BsaWI w'CCGG_w

Cuts at:      0     633     729 
   Size:        633      96
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     594     624     729 
   Size:        594      30     105

  Fragments arranged by size: 

                 594     105      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     370     729 
   Size:        370     359
 
Bsp1286I G_dGCh'C

Cuts at:      0      94     729 
   Size:         94     635
 
BstBI TT'CG_AA

Cuts at:      0     445     729 
   Size:        445     284
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     410     729 
   Size:        410     319
 
BtsI GCAGTG_nn'

Cuts at:      0     342     729 
   Size:        342     387
 
EarI CTCTTCn'nnn_

Cuts at:      0      75     347     729 
   Size:         75     272     382

  Fragments arranged by size: 

                 382     272      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     722     729 
   Size:        722       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0      91     729 
   Size:         91     638
 
EcoRI G'AATT_C

Cuts at:      0     156     729 
   Size:        156     573
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     205     237     525     557     594     626     682     714 
   Size:        205      32     288      32      37      32      56      32

Cuts at:    714     729 
   Size:         15

  Fragments arranged by size: 

                 288     205      56      37      32      32      32      32
                  15
 
MslI CAynn'nnrTG

Cuts at:      0     260     729 
   Size:        260     469
 
PfoI T'CCnGG_A

Cuts at:      0     374     729 
   Size:        374     355
 
PspOMI G'GGCC_C

Cuts at:      0      90     729 
   Size:         90     639
 
SfcI C'TryA_G

Cuts at:      0     527     729 
   Size:        527     202
 
XmnI GAAnn'nnTTC

Cuts at:      0      84     156     729 
   Size:         84      72     573

  Fragments arranged by size: 

                 573      84      72
 

 Enzymes that do cut:

     AcuI     AhdI     ApaI     ApoI     AvaI     BanI    BanII     BbsI
 Bme1580I     BplI    BsaHI    BsaWI    BsaXI    BseRI Bsp1286I    BstBI
    BtgZI     BtsI     EarI  Eco57MI EcoO109I    EcoRI    Hin4I     MslI
     PfoI   PspOMI     SfcI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI