Restriction map for
pF1KB3766
(Linear) (Six-base) MAPSORT of: KIBB3766.seq Check: 5173 from: 1 to: 1512
KIBB3766.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 845 1010 1455 1512
Size: 686 159 165 445 57
Fragments arranged by size:
686 445 165 159 57
AleI CACnn'nnGTG
Cuts at: 0 1257 1512
Size: 1257 255
AlwNI CAG_nnn'CTG
Cuts at: 0 1442 1512
Size: 1442 70
ApoI r'AATT_y
Cuts at: 0 1498 1512
Size: 1498 14
AvrII C'CTAG_G
Cuts at: 0 610 1512
Size: 610 902
BanI G'GyrC_C
Cuts at: 0 1262 1512
Size: 1262 250
BanII G_rGCy'C
Cuts at: 0 37 833 1512
Size: 37 796 679
Fragments arranged by size:
796 679 37
BclI T'GATC_A
Cuts at: 0 1116 1512
Size: 1116 396
BglI GCCn_nnn'nGGC
Cuts at: 0 32 1512
Size: 32 1480
BmtI G_CTAG'C
Cuts at: 0 160 1512
Size: 160 1352
Bpu10I CC'TnA_GC
Cuts at: 0 1097 1476 1512
Size: 1097 379 36
Fragments arranged by size:
1097 379 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 701 1512
Size: 701 811
BseYI C'CCAG_C
Cuts at: 0 30 1512
Size: 30 1482
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1512
Size: 251 1261
BsiHKAI G_wGCw'C
Cuts at: 0 395 833 1512
Size: 395 438 679
Fragments arranged by size:
679 438 395
BsmI GAATG_Cn'
Cuts at: 0 1026 1512
Size: 1026 486
Bsp1286I G_dGCh'C
Cuts at: 0 37 395 833 1512
Size: 37 358 438 679
Fragments arranged by size:
679 438 358 37
BspHI T'CATG_A
Cuts at: 0 881 1512
Size: 881 631
BsrDI GCAATG_nn'
Cuts at: 0 899 1512
Size: 899 613
BsrFI r'CCGG_y
Cuts at: 0 26 1512
Size: 26 1486
BstAPI GCAn_nnn'nTGC
Cuts at: 0 468 1512
Size: 468 1044
BstEII G'GTnAC_C
Cuts at: 0 196 1512
Size: 196 1316
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 1512
Size: 30 1482
BstYI r'GATC_y
Cuts at: 0 66 1386 1512
Size: 66 1320 126
Fragments arranged by size:
1320 126 66
BtsI GCAGTG_nn'
Cuts at: 0 311 939 1252 1264 1512
Size: 311 628 313 12 248
Fragments arranged by size:
628 313 311 248 12
DraI TTT'AAA
Cuts at: 0 384 1497 1512
Size: 384 1113 15
Fragments arranged by size:
1113 384 15
EarI CTCTTCn'nnn_
Cuts at: 0 821 1512
Size: 821 691
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1496 1512
Size: 1496 16
EcoICRI GAG'CTC
Cuts at: 0 831 1512
Size: 831 681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 845 1010 1455 1512
Size: 686 159 165 445 57
Fragments arranged by size:
686 445 165 159 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 286 324 376 1512
Size: 286 38 52 1136
Fragments arranged by size:
1136 286 52 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 186 1512
Size: 154 32 1326
Fragments arranged by size:
1326 154 32
MfeI C'AATT_G
Cuts at: 0 295 1512
Size: 295 1217
MslI CAynn'nnrTG
Cuts at: 0 1257 1512
Size: 1257 255
MspA1I CmG'CkG
Cuts at: 0 148 823 1330 1512
Size: 148 675 507 182
Fragments arranged by size:
675 507 182 148
NaeI GCC'GGC
Cuts at: 0 28 1512
Size: 28 1484
NgoMIV G'CCGG_C
Cuts at: 0 26 1512
Size: 26 1486
NheI G'CTAG_C
Cuts at: 0 156 1512
Size: 156 1356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 707 739 1297 1329 1512
Size: 707 32 558 32 183
Fragments arranged by size:
707 558 183 32 32
PsiI TTA'TAA
Cuts at: 0 445 595 677 1187 1512
Size: 445 150 82 510 325
Fragments arranged by size:
510 445 325 150 82
PstI C_TGCA'G
Cuts at: 0 74 318 1259 1512
Size: 74 244 941 253
Fragments arranged by size:
941 253 244 74
PvuII CAG'CTG
Cuts at: 0 148 823 1330 1512
Size: 148 675 507 182
Fragments arranged by size:
675 507 182 148
SacI G_AGCT'C
Cuts at: 0 833 1512
Size: 833 679
SapI GCTCTTCn'nnn_
Cuts at: 0 821 1512
Size: 821 691
SfcI C'TryA_G
Cuts at: 0 70 314 518 1255 1512
Size: 70 244 204 737 257
Fragments arranged by size:
737 257 244 204 70
StuI AGG'CCT
Cuts at: 0 1045 1512
Size: 1045 467
StyI C'CwwG_G
Cuts at: 0 610 1512
Size: 610 902
SwaI ATTT'AAAT
Cuts at: 0 1497 1512
Size: 1497 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1280 1512
Size: 1280 232
TatI w'GTAC_w
Cuts at: 0 692 1512
Size: 692 820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 703 735 1512
Size: 703 32 777
Fragments arranged by size:
777 703 32
XbaI T'CTAG_A
Cuts at: 0 1137 1512
Size: 1137 375
XmnI GAAnn'nnTTC
Cuts at: 0 1076 1312 1512
Size: 1076 236 200
Fragments arranged by size:
1076 236 200
Enzymes that do cut:
AcuI AleI AlwNI ApoI AvrII BanI BanII BclI
BglI BmtI Bpu10I BsaI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrFI BstAPI BstEII BstXI BstYI
BtsI DraI EarI EciI EcoICRI Eco57MI FalI Hin4I
MfeI MslI MspA1I NaeI NgoMIV NheI PpiI PsiI
PstI PvuII SacI SapI SfcI StuI StyI SwaI
TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MmeI MscI NarI NcoI NdeI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI Tth111I XcmI XhoI XmaI ZraI