Restriction map for
pF1KB3726
(Linear) (Six-base) MAPSORT of: KIBB3726.seq Check: 4134 from: 1 to: 2577
KIBB3726.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2285 2577
Size: 2285 292
AatII G_ACGT'C
Cuts at: 0 2480 2577
Size: 2480 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 379 781 1018 1240 1918 2577
Size: 48 331 402 237 222 678 659
Fragments arranged by size:
678 659 402 331 237 222 48
AfeI AGC'GCT
Cuts at: 0 1969 2577
Size: 1969 608
AflIII A'CryG_T
Cuts at: 0 680 2577
Size: 680 1897
AhdI GACnn_n'nnGTC
Cuts at: 0 1491 2466 2577
Size: 1491 975 111
Fragments arranged by size:
1491 975 111
AleI CACnn'nnGTG
Cuts at: 0 772 1831 2572 2577
Size: 772 1059 741 5
Fragments arranged by size:
1059 772 741 5
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 817 849 2577
Size: 817 32 1728
Fragments arranged by size:
1728 817 32
AlwNI CAG_nnn'CTG
Cuts at: 0 528 1692 2248 2577
Size: 528 1164 556 329
Fragments arranged by size:
1164 556 528 329
ApaI G_GGCC'C
Cuts at: 0 119 2577
Size: 119 2458
ApaLI G'TGCA_C
Cuts at: 0 1105 2577
Size: 1105 1472
ApoI r'AATT_y
Cuts at: 0 1861 1873 2577
Size: 1861 12 704
Fragments arranged by size:
1861 704 12
AvaI C'yCGr_G
Cuts at: 0 288 1078 2185 2577
Size: 288 790 1107 392
Fragments arranged by size:
1107 790 392 288
BamHI G'GATC_C
Cuts at: 0 1283 1429 1697 2577
Size: 1283 146 268 880
Fragments arranged by size:
1283 880 268 146
BanI G'GyrC_C
Cuts at: 0 1977 2577
Size: 1977 600
BanII G_rGCy'C
Cuts at: 0 119 130 943 1405 1415 1646 1684 2360
Size: 119 11 813 462 10 231 38 676
Cuts at: 2360 2577
Size: 217
Fragments arranged by size:
813 676 462 231 217 119 38 11
10
BbsI GAAGACnn'nnnn_
Cuts at: 0 954 1009 1923 2082 2577
Size: 954 55 914 159 495
Fragments arranged by size:
954 914 495 159 55
BglI GCCn_nnn'nGGC
Cuts at: 0 20 2577
Size: 20 2557
BglII A'GATC_T
Cuts at: 0 311 629 1148 2577
Size: 311 318 519 1429
Fragments arranged by size:
1429 519 318 311
BlpI GC'TnA_GC
Cuts at: 0 10 178 2577
Size: 10 168 2399
Fragments arranged by size:
2399 168 10
Bme1580I G_kGCm'C
Cuts at: 0 119 475 1109 1982 2577
Size: 119 356 634 873 595
Fragments arranged by size:
873 634 595 356 119
BmgBI CAC'GTC
Cuts at: 0 683 960 1833 2574 2577
Size: 683 277 873 741 3
Fragments arranged by size:
873 741 683 277 3
BmrI ACTGGGnnnn_n'
Cuts at: 0 1680 2577
Size: 1680 897
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 604 1150 1369 1399 1481 1597 1696
Size: 286 318 546 219 30 82 116 99
Cuts at: 1696 2577
Size: 881
Fragments arranged by size:
881 546 318 286 219 116 99 82
30
Bpu10I CC'TnA_GC
Cuts at: 0 1399 1444 2577
Size: 1399 45 1133
Fragments arranged by size:
1399 1133 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 2065 2146 2527 2577
Size: 1144 921 81 381 50
Fragments arranged by size:
1144 921 381 81 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 127 403 1804 2409 2577
Size: 127 276 1401 605 168
Fragments arranged by size:
1401 605 276 168 127
BsaBI GATnn'nnATC
Cuts at: 0 1147 2577
Size: 1147 1430
BsaHI Gr'CG_yC
Cuts at: 0 209 1928 2477 2577
Size: 209 1719 549 100
Fragments arranged by size:
1719 549 209 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1633 1663 2577
Size: 1633 30 914
Fragments arranged by size:
1633 914 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 1672 2020 2044 2185 2491 2577
Size: 118 1554 348 24 141 306 86
Fragments arranged by size:
1554 348 306 141 118 86 24
BseYI C'CCAG_C
Cuts at: 0 380 2214 2577
Size: 380 1834 363
Fragments arranged by size:
1834 380 363
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 2577
Size: 621 1956
BsiHKAI G_wGCw'C
Cuts at: 0 392 739 943 1109 1405 1415 1424 2577
Size: 392 347 204 166 296 10 9 1153
Fragments arranged by size:
1153 392 347 296 204 166 10 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 216 886 2577
Size: 216 670 1691
Fragments arranged by size:
1691 670 216
Bsp1286I G_dGCh'C
Cuts at: 0 119 130 392 475 739 943 1109 1405
Size: 119 11 262 83 264 204 166 296
Cuts at: 1405 1415 1424 1646 1684 1982 2360 2577
Size: 10 9 222 38 298 378 217
Fragments arranged by size:
378 298 296 264 262 222 217 204
166 119 83 38 11 10 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 764 2285 2478 2519 2577
Size: 764 1521 193 41 58
Fragments arranged by size:
1521 764 193 58 41
BsrBI CCG'CTC
Cuts at: 0 9 2193 2285 2577
Size: 9 2184 92 292
Fragments arranged by size:
2184 292 92 9
BsrFI r'CCGG_y
Cuts at: 0 1924 2577
Size: 1924 653
BsrGI T'GTAC_A
Cuts at: 0 548 1452 2577
Size: 548 904 1125
Fragments arranged by size:
1125 904 548
BstEII G'GTnAC_C
Cuts at: 0 501 2577
Size: 501 2076
BstYI r'GATC_y
Cuts at: 0 311 425 443 629 1148 1283 1380 1429
Size: 311 114 18 186 519 135 97 49
Cuts at: 1429 1697 1784 2577
Size: 268 87 793
Fragments arranged by size:
793 519 311 268 186 135 114 97
87 49 18
Bsu36I CC'TnA_GG
Cuts at: 0 227 1693 2577
Size: 227 1466 884
Fragments arranged by size:
1466 884 227
BtgI C'CryG_G
Cuts at: 0 506 692 1805 2063 2577
Size: 506 186 1113 258 514
Fragments arranged by size:
1113 514 506 258 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2133 2217 2577
Size: 2133 84 360
Fragments arranged by size:
2133 360 84
BtsI GCAGTG_nn'
Cuts at: 0 878 2002 2577
Size: 878 1124 575
Fragments arranged by size:
1124 878 575
ClaI AT'CG_AT
Cuts at: 0 149 2577
Size: 149 2428
DrdI GACnn_nn'nnGTC
Cuts at: 0 499 2577
Size: 499 2078
EaeI y'GGCC_r
Cuts at: 0 299 530 1055 2060 2577
Size: 299 231 525 1005 517
Fragments arranged by size:
1005 525 517 299 231
EarI CTCTTCn'nnn_
Cuts at: 0 302 523 1894 2011 2323 2577
Size: 302 221 1371 117 312 254
Fragments arranged by size:
1371 312 302 254 221 117
EcoICRI GAG'CTC
Cuts at: 0 941 1403 1413 2577
Size: 941 462 10 1164
Fragments arranged by size:
1164 941 462 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 286 379 604 781 1018 1150 1240
Size: 48 238 93 225 177 237 132 90
Cuts at: 1240 1369 1399 1481 1597 1696 1918 2577
Size: 129 30 82 116 99 222 659
Fragments arranged by size:
659 238 237 225 222 177 132 129
116 99 93 90 82 48 30
EcoO109I rG'GnC_Cy
Cuts at: 0 21 115 116 655 1904 2496 2577
Size: 21 94 1 539 1249 592 81
Fragments arranged by size:
1249 592 539 94 81 21 1
EcoRV GAT'ATC
Cuts at: 0 2051 2577
Size: 2051 526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 933 2106 2187 2577
Size: 933 1173 81 390
Fragments arranged by size:
1173 933 390 81
HaeII r_GCGC'y
Cuts at: 0 185 1218 1971 2080 2577
Size: 185 1033 753 109 497
Fragments arranged by size:
1033 753 497 185 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 1275 1307 1477 1509 2452 2484
Size: 196 32 1047 32 170 32 943 32
Cuts at: 2484 2577
Size: 93
Fragments arranged by size:
1047 943 196 170 93 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1737 2577
Size: 1737 840
HindIII A'AGCT_T
Cuts at: 0 1441 2577
Size: 1441 1136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 2577
Size: 635 1942
MscI TGG'CCA
Cuts at: 0 301 532 2062 2577
Size: 301 231 1530 515
Fragments arranged by size:
1530 515 301 231
MslI CAynn'nnrTG
Cuts at: 0 772 1141 1831 2572 2577
Size: 772 369 690 741 5
Fragments arranged by size:
772 741 690 369 5
MspA1I CmG'CkG
Cuts at: 0 327 592 795 903 1639 1791 2577
Size: 327 265 203 108 736 152 786
Fragments arranged by size:
786 736 327 265 203 152 108
NcoI C'CATG_G
Cuts at: 0 506 692 2577
Size: 506 186 1885
Fragments arranged by size:
1885 506 186
PflMI CCAn_nnn'nTGG
Cuts at: 0 528 1128 1820 2577
Size: 528 600 692 757
Fragments arranged by size:
757 692 600 528
PfoI T'CCnGG_A
Cuts at: 0 37 428 943 1497 1628 2174 2577
Size: 37 391 515 554 131 546 403
Fragments arranged by size:
554 546 515 403 391 131 37
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1228 1260 2577
Size: 1228 32 1317
Fragments arranged by size:
1317 1228 32
PpuMI rG'GwC_Cy
Cuts at: 0 655 1904 2496 2577
Size: 655 1249 592 81
Fragments arranged by size:
1249 655 592 81
PshAI GACnn'nnGTC
Cuts at: 0 1732 1927 2577
Size: 1732 195 650
Fragments arranged by size:
1732 650 195
PspOMI G'GGCC_C
Cuts at: 0 115 2577
Size: 115 2462
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2542 2574 2577
Size: 2542 32 3
Fragments arranged by size:
2542 32 3
PstI C_TGCA'G
Cuts at: 0 803 873 1014 2516 2577
Size: 803 70 141 1502 61
Fragments arranged by size:
1502 803 141 70 61
PvuII CAG'CTG
Cuts at: 0 327 592 903 1791 2577
Size: 327 265 311 888 786
Fragments arranged by size:
888 786 327 311 265
RsrII CG'GwC_CG
Cuts at: 0 2202 2577
Size: 2202 375
SacI G_AGCT'C
Cuts at: 0 943 1405 1415 2577
Size: 943 462 10 1162
Fragments arranged by size:
1162 943 462 10
SexAI A'CCwGG_T
Cuts at: 0 1061 2577
Size: 1061 1516
SfcI C'TryA_G
Cuts at: 0 54 799 869 1010 1369 2043 2512 2577
Size: 54 745 70 141 359 674 469 65
Fragments arranged by size:
745 674 469 359 141 70 65 54
SmlI C'TyrA_G
Cuts at: 0 1159 2080 2161 2542 2577
Size: 1159 921 81 381 35
Fragments arranged by size:
1159 921 381 81 35
StuI AGG'CCT
Cuts at: 0 263 1390 2577
Size: 263 1127 1187
Fragments arranged by size:
1187 1127 263
StyI C'CwwG_G
Cuts at: 0 476 506 659 668 692 884 1538 1730
Size: 476 30 153 9 24 192 654 192
Cuts at: 1730 1973 2255 2577
Size: 243 282 322
Fragments arranged by size:
654 476 322 282 243 192 192 153
30 24 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2219 2577
Size: 2219 358
TatI w'GTAC_w
Cuts at: 0 548 1452 1565 2168 2558 2577
Size: 548 904 113 603 390 19
Fragments arranged by size:
904 603 548 390 113 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1633 1665 2577
Size: 1633 32 912
Fragments arranged by size:
1633 912 32
Tth111I GACn'n_nGTC
Cuts at: 0 724 1908 2577
Size: 724 1184 669
Fragments arranged by size:
1184 724 669
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1294 2577
Size: 1294 1283
XmnI GAAnn'nnTTC
Cuts at: 0 1243 1358 2577
Size: 1243 115 1219
Fragments arranged by size:
1243 1219 115
ZraI GAC'GTC
Cuts at: 0 2478 2577
Size: 2478 99
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AleI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI DrdI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI
PflMI PfoI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuII RsrII SacI SexAI SfcI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AlfI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BmtI BplI BsaAI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BsrDI BssHII BssSI BstAPI BstBI BstXI
BstZ17I CspCI DraI DraIII EagI EciI EcoNI EcoRI
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PsiI PspXI
PvuI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI XmaI