(Linear) (Six-base) MAPSORT of: KIBB3668.seq  Check: 4559  from: 1  to: 783



KIBB3668.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AflIII A'CryG_T

Cuts at:      0     511     783 
   Size:        511     272
 
ApoI r'AATT_y

Cuts at:      0     200     317     764     783 
   Size:        200     117     447      19

  Fragments arranged by size: 

                 447     200     117      19
 
AvaI C'yCGr_G

Cuts at:      0      14     783 
   Size:         14     769
 
BanI G'GyrC_C

Cuts at:      0      25      90     783 
   Size:         25      65     693

  Fragments arranged by size: 

                 693      65      25
 
BbeI G_GCGC'C

Cuts at:      0      29     783 
   Size:         29     754
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     402     783 
   Size:        402     381
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      97     783 
   Size:         97     686
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     773     783 
   Size:        773      10
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     731     783 
   Size:        731      52
 
BsaHI Gr'CG_yC

Cuts at:      0      26     783 
   Size:         26     757
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     783 
   Size:        199     584
 
BsiHKAI G_wGCw'C

Cuts at:      0      65     783 
   Size:         65     718
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     783 
   Size:         65     718
 
BspHI T'CATG_A

Cuts at:      0     129     783 
   Size:        129     654
 
BsrDI GCAATG_nn'

Cuts at:      0     672     783 
   Size:        672     111
 
BtsI GCAGTG_nn'

Cuts at:      0     187     783 
   Size:        187     596
 
DraI TTT'AAA

Cuts at:      0     165     783 
   Size:        165     618
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     431     783 
   Size:        431     352
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     773     783 
   Size:        773      10
 
HaeII r_GCGC'y

Cuts at:      0      29     783 
   Size:         29     754
 
HindIII A'AGCT_T

Cuts at:      0     106     783 
   Size:        106     677
 
KasI G'GCGC_C

Cuts at:      0      25     783 
   Size:         25     758
 
MslI CAynn'nnrTG

Cuts at:      0     649     783 
   Size:        649     134
 
MspA1I CmG'CkG

Cuts at:      0      32      51     597     783 
   Size:         32      19     546     186

  Fragments arranged by size: 

                 546     186      32      19
 
NarI GG'CG_CC

Cuts at:      0      26     783 
   Size:         26     757
 
NspI r_CATG'y

Cuts at:      0     211     783 
   Size:        211     572
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     587     783 
   Size:        587     196
 
PvuII CAG'CTG

Cuts at:      0     597     783 
   Size:        597     186
 
SexAI A'CCwGG_T

Cuts at:      0     580     783 
   Size:        580     203
 
SfcI C'TryA_G

Cuts at:      0     540     783 
   Size:        540     243
 
SfoI GGC'GCC

Cuts at:      0      27     783 
   Size:         27     756
 
SmaI CCC'GGG

Cuts at:      0      16     783 
   Size:         16     767
 
SmlI C'TyrA_G

Cuts at:      0     746     783 
   Size:        746      37
 
SspI AAT'ATT

Cuts at:      0     253     783 
   Size:        253     530
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     166     198     783 
   Size:        166      32     585

  Fragments arranged by size: 

                 585     166      32
 
XmaI C'CCGG_G

Cuts at:      0      14     783 
   Size:         14     769
 
XmnI GAAnn'nnTTC

Cuts at:      0     200     783 
   Size:        200     583
 

 Enzymes that do cut:

   AflIII     ApoI     AvaI     BanI     BbeI     BbsI     BplI     BpmI
    BpuEI    BsaHI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrDI     BtsI
     DraI     DrdI  Eco57MI    HaeII  HindIII     KasI     MslI   MspA1I
     NarI     NspI    PflMI    PvuII    SexAI     SfcI     SfoI     SmaI
     SmlI     SspI     TstI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     ZraI