Restriction map for
pF1KB3647
(Linear) (Six-base) MAPSORT of: KIBB3647.seq Check: 8193 from: 1 to: 1794
KIBB3647.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 554 1794
Size: 554 1240
AatII G_ACGT'C
Cuts at: 0 1430 1794
Size: 1430 364
AccI GT'mk_AC
Cuts at: 0 1046 1794
Size: 1046 748
Acc65I G'GTAC_C
Cuts at: 0 1343 1794
Size: 1343 451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 338 571 616 1794
Size: 211 127 233 45 1178
Fragments arranged by size:
1178 233 211 127 45
AfeI AGC'GCT
Cuts at: 0 1651 1794
Size: 1651 143
AleI CACnn'nnGTG
Cuts at: 0 1535 1794
Size: 1535 259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1553 1794
Size: 1553 241
AlwNI CAG_nnn'CTG
Cuts at: 0 1614 1794
Size: 1614 180
ApaLI G'TGCA_C
Cuts at: 0 514 1794
Size: 514 1280
ApoI r'AATT_y
Cuts at: 0 380 748 1332 1794
Size: 380 368 584 462
Fragments arranged by size:
584 462 380 368
AvaI C'yCGr_G
Cuts at: 0 634 1794
Size: 634 1160
BamHI G'GATC_C
Cuts at: 0 1029 1794
Size: 1029 765
BanI G'GyrC_C
Cuts at: 0 1343 1537 1794
Size: 1343 194 257
Fragments arranged by size:
1343 257 194
BanII G_rGCy'C
Cuts at: 0 260 434 1794
Size: 260 174 1360
Fragments arranged by size:
1360 260 174
BbsI GAAGACnn'nnnn_
Cuts at: 0 1015 1794
Size: 1015 779
BbvCI CC'TCA_GC
Cuts at: 0 151 1205 1794
Size: 151 1054 589
Fragments arranged by size:
1054 589 151
BciVI GTATCCnnnnn_n'
Cuts at: 0 1484 1794
Size: 1484 310
BglI GCCn_nnn'nGGC
Cuts at: 0 142 1730 1794
Size: 142 1588 64
Fragments arranged by size:
1588 142 64
BlpI GC'TnA_GC
Cuts at: 0 767 1794
Size: 767 1027
Bme1580I G_kGCm'C
Cuts at: 0 518 1542 1693 1794
Size: 518 1024 151 101
Fragments arranged by size:
1024 518 151 101
BmrI ACTGGGnnnn_n'
Cuts at: 0 1078 1794
Size: 1078 716
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1208 1794
Size: 1208 586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1282 1471 1726 1794
Size: 229 1053 189 255 68
Fragments arranged by size:
1053 255 229 189 68
Bpu10I CC'TnA_GC
Cuts at: 0 151 332 1205 1794
Size: 151 181 873 589
Fragments arranged by size:
873 589 181 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1794
Size: 418 1376
BsaI GGTCTCn'nnnn_
Cuts at: 0 935 1794
Size: 935 859
BsaAI yAC'GTr
Cuts at: 0 744 1794
Size: 744 1050
BsaBI GATnn'nnATC
Cuts at: 0 463 1794
Size: 463 1331
BsaHI Gr'CG_yC
Cuts at: 0 110 935 1427 1794
Size: 110 825 492 367
Fragments arranged by size:
825 492 367 110
BsaWI w'CCGG_w
Cuts at: 0 402 797 1794
Size: 402 395 997
Fragments arranged by size:
997 402 395
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 883 913 1794
Size: 421 30 432 30 881
Fragments arranged by size:
881 432 421 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 604 625 1067 1549 1794
Size: 604 21 442 482 245
Fragments arranged by size:
604 482 442 245 21
BseYI C'CCAG_C
Cuts at: 0 761 887 966 1487 1607 1794
Size: 761 126 79 521 120 187
Fragments arranged by size:
761 521 187 126 120 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1513 1740 1794
Size: 874 639 227 54
Fragments arranged by size:
874 639 227 54
BsiEI CG_ry'CG
Cuts at: 0 130 1794
Size: 130 1664
BsiHKAI G_wGCw'C
Cuts at: 0 68 434 518 1794
Size: 68 366 84 1276
Fragments arranged by size:
1276 366 84 68
BsmI GAATG_Cn'
Cuts at: 0 1194 1794
Size: 1194 600
BsmBI CGTCTCn'nnnn_
Cuts at: 0 101 1794
Size: 101 1693
Bsp1286I G_dGCh'C
Cuts at: 0 68 260 434 518 1542 1693 1794
Size: 68 192 174 84 1024 151 101
Fragments arranged by size:
1024 192 174 151 101 84 68
BspEI T'CCGG_A
Cuts at: 0 402 1794
Size: 402 1392
BspHI T'CATG_A
Cuts at: 0 1679 1794
Size: 1679 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 458 554 708 1262 1794
Size: 458 96 154 554 532
Fragments arranged by size:
554 532 458 154 96
BsrBI CCG'CTC
Cuts at: 0 1140 1794
Size: 1140 654
BsrFI r'CCGG_y
Cuts at: 0 55 83 224 771 1794
Size: 55 28 141 547 1023
Fragments arranged by size:
1023 547 141 55 28
BsrGI T'GTAC_A
Cuts at: 0 905 1238 1643 1794
Size: 905 333 405 151
Fragments arranged by size:
905 405 333 151
BssHII G'CGCG_C
Cuts at: 0 132 155 1794
Size: 132 23 1639
Fragments arranged by size:
1639 132 23
BssSI C'ACGA_G
Cuts at: 0 118 1794
Size: 118 1676
BstXI CCAn_nnnn'nTGG
Cuts at: 0 926 1794
Size: 926 868
BstYI r'GATC_y
Cuts at: 0 122 1029 1794
Size: 122 907 765
Fragments arranged by size:
907 765 122
BstZ17I GTA'TAC
Cuts at: 0 1047 1794
Size: 1047 747
BtgI C'CryG_G
Cuts at: 0 876 1482 1794
Size: 876 606 312
Fragments arranged by size:
876 606 312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 75 1794
Size: 75 1719
DraI TTT'AAA
Cuts at: 0 1322 1794
Size: 1322 472
DraIII CAC_nnn'GTG
Cuts at: 0 422 1794
Size: 422 1372
EaeI y'GGCC_r
Cuts at: 0 74 127 143 1794
Size: 74 53 16 1651
Fragments arranged by size:
1651 74 53 16
EagI C'GGCC_G
Cuts at: 0 127 1794
Size: 127 1667
EarI CTCTTCn'nnn_
Cuts at: 0 9 73 581 658 1199 1296 1794
Size: 9 64 508 77 541 97 498
Fragments arranged by size:
541 508 498 97 77 64 9
EcoICRI GAG'CTC
Cuts at: 0 432 1794
Size: 432 1362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 229 338 571 616 1282 1471 1726
Size: 211 18 109 233 45 666 189 255
Cuts at: 1726 1794
Size: 68
Fragments arranged by size:
666 255 233 211 189 109 68 45
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 550 1794
Size: 550 1244
EcoO109I rG'GnC_Cy
Cuts at: 0 291 337 447 489 600 962 1107 1722
Size: 291 46 110 42 111 362 145 615
Cuts at: 1722 1794
Size: 72
Fragments arranged by size:
615 362 291 145 111 110 72 46
42
EcoRI G'AATT_C
Cuts at: 0 748 1794
Size: 748 1046
EcoRV GAT'ATC
Cuts at: 0 461 1794
Size: 461 1333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 310 1539 1794
Size: 310 1229 255
Fragments arranged by size:
1229 310 255
FspI TGC'GCA
Cuts at: 0 100 1794
Size: 100 1694
HaeII r_GCGC'y
Cuts at: 0 167 198 1653 1794
Size: 167 31 1455 141
Fragments arranged by size:
1455 167 141 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 669 701 1213 1245 1794
Size: 478 32 159 32 512 32 549
Fragments arranged by size:
549 512 478 159 32 32 32
HincII GTy'rAC
Cuts at: 0 996 1794
Size: 996 798
KpnI G_GTAC'C
Cuts at: 0 1347 1794
Size: 1347 447
MscI TGG'CCA
Cuts at: 0 76 145 1794
Size: 76 69 1649
Fragments arranged by size:
1649 76 69
MslI CAynn'nnrTG
Cuts at: 0 94 478 565 1535 1794
Size: 94 384 87 970 259
Fragments arranged by size:
970 384 259 94 87
MspA1I CmG'CkG
Cuts at: 0 104 231 757 858 887 1260 1449 1574
Size: 104 127 526 101 29 373 189 125
Cuts at: 1574 1754 1794
Size: 180 40
Fragments arranged by size:
526 373 189 180 127 125 104 101
40 29
NaeI GCC'GGC
Cuts at: 0 57 85 1794
Size: 57 28 1709
Fragments arranged by size:
1709 57 28
NcoI C'CATG_G
Cuts at: 0 1482 1794
Size: 1482 312
NgoMIV G'CCGG_C
Cuts at: 0 55 83 1794
Size: 55 28 1711
Fragments arranged by size:
1711 55 28
NspI r_CATG'y
Cuts at: 0 906 1794
Size: 906 888
PasI CC'CwG_GG
Cuts at: 0 1542 1794
Size: 1542 252
PflMI CCAn_nnn'nTGG
Cuts at: 0 1614 1794
Size: 1614 180
PfoI T'CCnGG_A
Cuts at: 0 176 788 1269 1613 1794
Size: 176 612 481 344 181
Fragments arranged by size:
612 481 344 181 176
PmlI CAC'GTG
Cuts at: 0 744 1794
Size: 744 1050
PpuMI rG'GwC_Cy
Cuts at: 0 337 447 600 962 1107 1794
Size: 337 110 153 362 145 687
Fragments arranged by size:
687 362 337 153 145 110
PvuII CAG'CTG
Cuts at: 0 231 887 1754 1794
Size: 231 656 867 40
Fragments arranged by size:
867 656 231 40
RsrII CG'GwC_CG
Cuts at: 0 509 1794
Size: 509 1285
SacI G_AGCT'C
Cuts at: 0 434 1794
Size: 434 1360
SanDI GG'GwC_CC
Cuts at: 0 337 1794
Size: 337 1457
SapI GCTCTTCn'nnn_
Cuts at: 0 73 1199 1296 1794
Size: 73 1126 97 498
Fragments arranged by size:
1126 498 97 73
ScaI AGT'ACT
Cuts at: 0 439 1794
Size: 439 1355
SmaI CCC'GGG
Cuts at: 0 636 1794
Size: 636 1158
SmlI C'TyrA_G
Cuts at: 0 433 1794
Size: 433 1361
SspI AAT'ATT
Cuts at: 0 1062 1469 1794
Size: 1062 407 325
Fragments arranged by size:
1062 407 325
StuI AGG'CCT
Cuts at: 0 674 1794
Size: 674 1120
StyI C'CwwG_G
Cuts at: 0 252 443 1482 1514 1794
Size: 252 191 1039 32 280
Fragments arranged by size:
1039 280 252 191 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1525 1794
Size: 1525 269
TatI w'GTAC_w
Cuts at: 0 437 905 1238 1643 1794
Size: 437 468 333 405 151
Fragments arranged by size:
468 437 405 333 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 883 915 1794
Size: 883 32 879
Fragments arranged by size:
883 879 32
Tth111I GACn'n_nGTC
Cuts at: 0 1627 1794
Size: 1627 167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 349 926 1794
Size: 349 577 868
Fragments arranged by size:
868 577 349
XmaI C'CCGG_G
Cuts at: 0 634 1794
Size: 634 1160
XmnI GAAnn'nnTTC
Cuts at: 0 560 1006 1794
Size: 560 446 788
Fragments arranged by size:
788 560 446
ZraI GAC'GTC
Cuts at: 0 1428 1794
Size: 1428 366
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AleI AlfI
AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BciVI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstXI BstYI BstZ17I BtgI BtgZI DraI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KpnI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpuMI PvuII RsrII SacI SanDI SapI ScaI
SmaI SmlI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AflIII AgeI AhdI AloI ApaI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI
BglII BmgBI BmtI BsiWI BsrDI BstAPI BstBI BstEII
Bsu36I BtsI ClaI CspCI DrdI EciI FseI FspAI
HindIII HpaI KasI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacII
SalI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SwaI XbaI XhoI