(Linear) (Six-base) MAPSORT of: KIBB3606.seq  Check: 9234  from: 1  to: 594



KIBB3606.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AleI CACnn'nnGTG

Cuts at:      0      55     145     594 
   Size:         55      90     449

  Fragments arranged by size: 

                 449      90      55
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     223     255     594 
   Size:        223      32     339

  Fragments arranged by size: 

                 339     223      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      37     594 
   Size:         37     557
 
ApaI G_GGCC'C

Cuts at:      0     292     594 
   Size:        292     302
 
BanI G'GyrC_C

Cuts at:      0      67     594 
   Size:         67     527
 
BanII G_rGCy'C

Cuts at:      0     292     594 
   Size:        292     302
 
BbeI G_GCGC'C

Cuts at:      0      71     594 
   Size:         71     523
 
Bme1580I G_kGCm'C

Cuts at:      0     154     292     594 
   Size:        154     138     302

  Fragments arranged by size: 

                 302     154     138
 
BmgBI CAC'GTC

Cuts at:      0     318     594 
   Size:        318     276
 
BsaAI yAC'GTr

Cuts at:      0     111     594 
   Size:        111     483
 
BsaHI Gr'CG_yC

Cuts at:      0      68     221     594 
   Size:         68     153     373

  Fragments arranged by size: 

                 373     153      68
 
BsaWI w'CCGG_w

Cuts at:      0       7     594 
   Size:          7     587
 
BseYI C'CCAG_C

Cuts at:      0     201     216     594 
   Size:        201      15     378

  Fragments arranged by size: 

                 378     201      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     594 
   Size:        203     391
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     228     594 
   Size:        228     366
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     292     594 
   Size:        154     138     302

  Fragments arranged by size: 

                 302     154     138
 
BsrDI GCAATG_nn'

Cuts at:      0     368     594 
   Size:        368     226
 
BssHII G'CGCG_C

Cuts at:      0      16     594 
   Size:         16     578
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      57     594 
   Size:         57     537
 
BtgI C'CryG_G

Cuts at:      0     452     594 
   Size:        452     142
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     150     594 
   Size:        150     444
 
BtsI GCAGTG_nn'

Cuts at:      0     543     594 
   Size:        543      51
 
ClaI AT'CG_AT

Cuts at:      0     395     594 
   Size:        395     199
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     235     270     594 
   Size:        235      35     324

  Fragments arranged by size: 

                 324     235      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     289     381     594 
   Size:        289      92     213

  Fragments arranged by size: 

                 289     213      92
 
HaeII r_GCGC'y

Cuts at:      0      71     594 
   Size:         71     523
 
KasI G'GCGC_C

Cuts at:      0      67     594 
   Size:         67     527
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     594 
   Size:         71     523
 
MslI CAynn'nnrTG

Cuts at:      0      55     145     594 
   Size:         55      90     449

  Fragments arranged by size: 

                 449      90      55
 
MspA1I CmG'CkG

Cuts at:      0      55     520     594 
   Size:         55     465      74

  Fragments arranged by size: 

                 465      74      55
 
NarI GG'CG_CC

Cuts at:      0      68     594 
   Size:         68     526
 
PshAI GACnn'nnGTC

Cuts at:      0     330     594 
   Size:        330     264
 
PspOMI G'GGCC_C

Cuts at:      0     288     594 
   Size:        288     306
 
SfcI C'TryA_G

Cuts at:      0     375     594 
   Size:        375     219
 
SfoI GGC'GCC

Cuts at:      0      69     594 
   Size:         69     525
 
SspI AAT'ATT

Cuts at:      0     577     594 
   Size:        577      17
 
StuI AGG'CCT

Cuts at:      0     481     594 
   Size:        481     113
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     259     594 
   Size:        259     335
 
TatI w'GTAC_w

Cuts at:      0     488     594 
   Size:        488     106
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     437     469     594 
   Size:        437      32     125

  Fragments arranged by size: 

                 437     125      32
 

 Enzymes that do cut:

     AleI     AloI    AlwNI     ApaI     BanI    BanII     BbeI Bme1580I
    BmgBI    BsaAI    BsaHI    BsaWI    BseYI     BsgI    BsmBI Bsp1286I
    BsrDI   BssHII    BstXI     BtgI    BtgZI     BtsI     ClaI    CspCI
 EcoO109I    HaeII     KasI     MmeI     MslI   MspA1I     NarI    PshAI
   PspOMI     SfcI     SfoI     SspI     StuI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI    BseRI
    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StyI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI