Restriction map for
pF1KB3598
(Linear) (Six-base) MAPSORT of: KIBB3598.seq Check: 4217 from: 1 to: 4497
KIBB3598.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AatII G_ACGT'C
Cuts at: 0 1686 4497
Size: 1686 2811
Acc65I G'GTAC_C
Cuts at: 0 1312 3166 4497
Size: 1312 1854 1331
Fragments arranged by size:
1854 1331 1312
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1211 1634 1769 2381 3433 3533 3776
Size: 913 298 423 135 612 1052 100 243
Cuts at: 3776 3835 4497
Size: 59 662
Fragments arranged by size:
1052 913 662 612 423 298 243 135
100 59
AflIII A'CryG_T
Cuts at: 0 3883 4003 4497
Size: 3883 120 494
Fragments arranged by size:
3883 494 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 710 4497
Size: 710 3787
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1107 1139 1521 1553 2691 2723 4497
Size: 1107 32 382 32 1138 32 1774
Fragments arranged by size:
1774 1138 1107 382 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1594 2764 2782 3628 4497
Size: 1594 1170 18 846 869
Fragments arranged by size:
1594 1170 869 846 18
ApaI G_GGCC'C
Cuts at: 0 775 1226 1972 2413 3107 3637 4497
Size: 775 451 746 441 694 530 860
Fragments arranged by size:
860 775 746 694 530 451 441
ApaLI G'TGCA_C
Cuts at: 0 1070 4497
Size: 1070 3427
ApoI r'AATT_y
Cuts at: 0 315 1385 4462 4497
Size: 315 1070 3077 35
Fragments arranged by size:
3077 1070 315 35
AvaI C'yCGr_G
Cuts at: 0 1712 1831 2459 2486 2504 2803 3314 4497
Size: 1712 119 628 27 18 299 511 1183
Fragments arranged by size:
1712 1183 628 511 299 119 27 18
AvrII C'CTAG_G
Cuts at: 0 2793 4497
Size: 2793 1704
BamHI G'GATC_C
Cuts at: 0 1674 2644 4497
Size: 1674 970 1853
Fragments arranged by size:
1853 1674 970
BanI G'GyrC_C
Cuts at: 0 988 1285 1312 3166 3247 4497
Size: 988 297 27 1854 81 1250
Fragments arranged by size:
1854 1250 988 297 81 27
BanII G_rGCy'C
Cuts at: 0 775 858 876 1226 1306 1929 1972 2413
Size: 775 83 18 350 80 623 43 441
Cuts at: 2413 2775 3107 3637 4497
Size: 362 332 530 860
Fragments arranged by size:
860 775 623 530 441 362 350 332
83 80 43 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 92 456 874 1243 2269 2386 2818 3439
Size: 92 364 418 369 1026 117 432 621
Cuts at: 3439 3448 4497
Size: 9 1049
Fragments arranged by size:
1049 1026 621 432 418 369 364 117
92 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 999 1033 4497
Size: 999 34 3464
Fragments arranged by size:
3464 999 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1109 4222 4497
Size: 1109 3113 275
Fragments arranged by size:
3113 1109 275
BclI T'GATC_A
Cuts at: 0 2079 3393 4497
Size: 2079 1314 1104
Fragments arranged by size:
2079 1314 1104
BglI GCCn_nnn'nGGC
Cuts at: 0 1301 1850 2228 2480 3029 3434 3579 3659
Size: 1301 549 378 252 549 405 145 80
Cuts at: 3659 3857 4497
Size: 198 640
Fragments arranged by size:
1301 640 549 549 405 378 252 198
145 80
BglII A'GATC_T
Cuts at: 0 179 4425 4497
Size: 179 4246 72
Fragments arranged by size:
4246 179 72
BlpI GC'TnA_GC
Cuts at: 0 1332 4497
Size: 1332 3165
Bme1580I G_kGCm'C
Cuts at: 0 775 1074 1226 1972 2413 3107 3252 3637
Size: 775 299 152 746 441 694 145 385
Cuts at: 3637 3716 4497
Size: 79 781
Fragments arranged by size:
781 775 746 694 441 385 299 152
145 79
BmrI ACTGGGnnnn_n'
Cuts at: 0 21 1229 3532 4497
Size: 21 1208 2303 965
Fragments arranged by size:
2303 1208 965 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 223 1943 4497
Size: 223 1720 2554
Fragments arranged by size:
2554 1720 223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 392 500 1229 1337 1526 1787 1907
Size: 320 72 108 729 108 189 261 120
Cuts at: 1907 1925 1994 2030 2078 2114 2222 2276 2399
Size: 18 69 36 48 36 108 54 123
Cuts at: 2399 2456 2546 2591 2609 2741 2759 2789 2804
Size: 57 90 45 18 132 18 30 15
Cuts at: 2804 3263 3515 3578 4497
Size: 459 252 63 919
Fragments arranged by size:
919 729 459 320 261 252 189 132
123 120 108 108 108 90 72 69
63 57 54 48 45 36 36 30
18 18 18 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 1607 4497
Size: 579 1028 2890
Fragments arranged by size:
2890 1028 579
BsaI GGTCTCn'nnnn_
Cuts at: 0 3206 3395 4112 4118 4497
Size: 3206 189 717 6 379
Fragments arranged by size:
3206 717 379 189 6
BsaAI yAC'GTr
Cuts at: 0 382 760 3884 4036 4497
Size: 382 378 3124 152 461
Fragments arranged by size:
3124 461 382 378 152
BsaHI Gr'CG_yC
Cuts at: 0 1683 4497
Size: 1683 2814
BsaWI w'CCGG_w
Cuts at: 0 4322 4497
Size: 4322 175
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 696 726 1290 1320 4497
Size: 696 30 564 30 3177
Fragments arranged by size:
3177 696 564 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 443 2009 2144 2171 3314 4497
Size: 22 421 1566 135 27 1143 1183
Fragments arranged by size:
1566 1183 1143 421 135 27 22
BseYI C'CCAG_C
Cuts at: 0 586 3033 3343 3873 4497
Size: 586 2447 310 530 624
Fragments arranged by size:
2447 624 586 530 310
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 716 4497
Size: 105 611 3781
Fragments arranged by size:
3781 611 105
BsiEI CG_ry'CG
Cuts at: 0 2966 4497
Size: 2966 1531
BsiHKAI G_wGCw'C
Cuts at: 0 516 858 948 1056 1074 1569 1623 1713
Size: 516 342 90 108 18 495 54 90
Cuts at: 1713 1929 2775 3279 4497
Size: 216 846 504 1218
Fragments arranged by size:
1218 846 516 504 495 342 216 108
90 90 54 18
BsmI GAATG_Cn'
Cuts at: 0 229 277 1022 4497
Size: 229 48 745 3475
Fragments arranged by size:
3475 745 229 48
Bsp1286I G_dGCh'C
Cuts at: 0 516 775 858 876 948 1056 1074 1226
Size: 516 259 83 18 72 108 18 152
Cuts at: 1226 1306 1569 1623 1713 1929 1972 2413 2775
Size: 80 263 54 90 216 43 441 362
Cuts at: 2775 3107 3252 3279 3637 3716 4497
Size: 332 145 27 358 79 781
Fragments arranged by size:
781 516 441 362 358 332 263 259
216 152 145 108 90 83 80 79
72 54 43 27 18 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 174 1156 2416 2790 3217 4497
Size: 174 982 1260 374 427 1280
Fragments arranged by size:
1280 1260 982 427 374 174
BsrDI GCAATG_nn'
Cuts at: 0 3165 4497
Size: 3165 1332
BsrFI r'CCGG_y
Cuts at: 0 396 4497
Size: 396 4101
BssSI C'ACGA_G
Cuts at: 0 461 857 3377 4497
Size: 461 396 2520 1120
Fragments arranged by size:
2520 1120 461 396
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1900 4497
Size: 1900 2597
BstEII G'GTnAC_C
Cuts at: 0 898 1519 1870 4497
Size: 898 621 351 2627
Fragments arranged by size:
2627 898 621 351
BstYI r'GATC_y
Cuts at: 0 179 844 1348 1674 2097 2644 3303 3735
Size: 179 665 504 326 423 547 659 432
Cuts at: 3735 3952 4425 4497
Size: 217 473 72
Fragments arranged by size:
665 659 547 504 473 432 423 326
217 179 72
Bsu36I CC'TnA_GG
Cuts at: 0 470 1199 1361 1379 3629 4497
Size: 470 729 162 18 2250 868
Fragments arranged by size:
2250 868 729 470 162 18
BtgI C'CryG_G
Cuts at: 0 992 4497
Size: 992 3505
BtsI GCAGTG_nn'
Cuts at: 0 1872 3615 4497
Size: 1872 1743 882
Fragments arranged by size:
1872 1743 882
DraIII CAC_nnn'GTG
Cuts at: 0 1051 1492 4497
Size: 1051 441 3005
Fragments arranged by size:
3005 1051 441
DrdI GACnn_nn'nnGTC
Cuts at: 0 614 743 914 1103 2552 2822 3146 3335
Size: 614 129 171 189 1449 270 324 189
Cuts at: 3335 4497
Size: 1162
Fragments arranged by size:
1449 1162 614 324 270 189 189 171
129
EaeI y'GGCC_r
Cuts at: 0 132 1788 3678 4497
Size: 132 1656 1890 819
Fragments arranged by size:
1890 1656 819 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 31 2699 4497
Size: 31 2668 1798
Fragments arranged by size:
2668 1798 31
EcoICRI GAG'CTC
Cuts at: 0 856 1927 2773 4497
Size: 856 1071 846 1724
Fragments arranged by size:
1724 1071 856 846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 392 500 913 1211 1229 1337 1526
Size: 320 72 108 413 298 18 108 189
Cuts at: 1526 1634 1769 1787 1907 1925 1994 2030 2078
Size: 108 135 18 120 18 69 36 48
Cuts at: 2078 2114 2222 2276 2381 2399 2456 2546 2591
Size: 36 108 54 105 18 57 90 45
Cuts at: 2591 2609 2741 2759 2789 2804 3263 3433 3515
Size: 18 132 18 30 15 459 170 82
Cuts at: 3515 3533 3578 3776 3835 4497
Size: 18 45 198 59 662
Fragments arranged by size:
662 459 413 320 298 198 189 170
135 132 120 108 108 108 108 105
90 82 72 69 59 57 54 48
45 45 36 36 30 18 18 18
18 18 18 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2060 3947 4497
Size: 2060 1887 550
Fragments arranged by size:
2060 1887 550
EcoO109I rG'GnC_Cy
Cuts at: 0 403 520 547 646 700 709 771 772
Size: 403 117 27 99 54 9 62 1
Cuts at: 772 925 997 1105 1168 1186 1195 1223 1339
Size: 153 72 108 63 18 9 28 116
Cuts at: 1339 1357 1510 1636 1654 1744 1798 1852 2005
Size: 18 153 126 18 90 54 54 153
Cuts at: 2005 2014 2050 2122 2249 2401 2410 2428 2554
Size: 9 36 72 127 152 9 18 126
Cuts at: 2554 2761 2779 2788 2815 2835 2907 3103 3104
Size: 207 18 9 27 20 72 196 1
Cuts at: 3104 3319 3373 3526 3625 3633 3634 3670 4497
Size: 215 54 153 99 8 1 36 827
Fragments arranged by size:
827 403 215 207 196 153 153 153
153 152 127 126 126 117 116 108
99 99 90 72 72 72 63 62
54 54 54 54 36 36 28 27
27 20 18 18 18 18 18 9
9 9 9 9 8 1 1 1
EcoRI G'AATT_C
Cuts at: 0 1385 4462 4497
Size: 1385 3077 35
Fragments arranged by size:
3077 1385 35
EcoRV GAT'ATC
Cuts at: 0 3705 4296 4497
Size: 3705 591 201
Fragments arranged by size:
3705 591 201
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1774 4398 4497
Size: 1774 2624 99
Fragments arranged by size:
2624 1774 99
FspI TGC'GCA
Cuts at: 0 3847 4497
Size: 3847 650
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 586 618 1713 1745 2827 2859
Size: 17 32 537 32 1095 32 1082 32
Cuts at: 2859 4117 4149 4333 4365 4497
Size: 1258 32 184 32 132
Fragments arranged by size:
1258 1095 1082 537 184 132 32 32
32 32 32 32 17
HincII GTy'rAC
Cuts at: 0 505 4497
Size: 505 3992
HindIII A'AGCT_T
Cuts at: 0 3898 4497
Size: 3898 599
KpnI G_GTAC'C
Cuts at: 0 1316 3170 4497
Size: 1316 1854 1327
Fragments arranged by size:
1854 1327 1316
MfeI C'AATT_G
Cuts at: 0 3584 4497
Size: 3584 913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 921 2730 3171 4497
Size: 572 349 1809 441 1326
Fragments arranged by size:
1809 1326 572 441 349
MscI TGG'CCA
Cuts at: 0 134 3680 4497
Size: 134 3546 817
Fragments arranged by size:
3546 817 134
MslI CAynn'nnrTG
Cuts at: 0 2852 3719 4497
Size: 2852 867 778
Fragments arranged by size:
2852 867 778
MspA1I CmG'CkG
Cuts at: 0 2371 2875 2956 3037 3154 3691 4497
Size: 2371 504 81 81 117 537 806
Fragments arranged by size:
2371 806 537 504 117 81 81
NcoI C'CATG_G
Cuts at: 0 992 4497
Size: 992 3505
NspI r_CATG'y
Cuts at: 0 2991 3018 3732 4007 4497
Size: 2991 27 714 275 490
Fragments arranged by size:
2991 714 490 275 27
PasI CC'CwG_GG
Cuts at: 0 265 1876 2550 3217 3568 3796 4497
Size: 265 1611 674 667 351 228 701
Fragments arranged by size:
1611 701 674 667 351 265 228
PciI A'CATG_T
Cuts at: 0 4003 4497
Size: 4003 494
PfoI T'CCnGG_A
Cuts at: 0 390 408 516 525 732 849 1056 1137
Size: 390 18 108 9 207 117 207 81
Cuts at: 1137 1146 1344 1353 1407 1677 1929 2055 2091
Size: 9 198 9 54 270 252 126 36
Cuts at: 2091 2100 2622 2676 2775 3279 4497
Size: 9 522 54 99 504 1218
Fragments arranged by size:
1218 522 504 390 270 252 207 207
198 126 117 108 99 81 54 54
36 18 9 9 9 9
PmlI CAC'GTG
Cuts at: 0 760 3884 4497
Size: 760 3124 613
Fragments arranged by size:
3124 760 613
PpuMI rG'GwC_Cy
Cuts at: 0 403 520 547 646 700 709 997 1105
Size: 403 117 27 99 54 9 288 108
Cuts at: 1105 1168 1195 1357 1510 1636 1654 1744 1798
Size: 63 27 162 153 126 18 90 54
Cuts at: 1798 2005 2122 2249 2401 2428 2554 2779 2788
Size: 207 117 127 152 27 126 225 9
Cuts at: 2788 2815 2835 2907 3319 3373 3526 3625 3670
Size: 27 20 72 412 54 153 99 45
Cuts at: 3670 4497
Size: 827
Fragments arranged by size:
827 412 403 288 225 207 162 153
153 152 127 126 126 117 117 108
99 99 90 72 63 54 54 54
45 27 27 27 27 20 18 9
9
PshAI GACnn'nnGTC
Cuts at: 0 534 3416 4497
Size: 534 2882 1081
Fragments arranged by size:
2882 1081 534
PspOMI G'GGCC_C
Cuts at: 0 771 1222 1968 2409 3103 3633 4497
Size: 771 451 746 441 694 530 864
Fragments arranged by size:
864 771 746 694 530 451 441
PspXI vC'TCGA_Gb
Cuts at: 0 2459 4497
Size: 2459 2038
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1260 1292 4189 4221 4497
Size: 1260 32 2897 32 276
Fragments arranged by size:
2897 1260 276 32 32
PstI C_TGCA'G
Cuts at: 0 3228 4497
Size: 3228 1269
PvuII CAG'CTG
Cuts at: 0 2371 2875 2956 3154 3691 4497
Size: 2371 504 81 198 537 806
Fragments arranged by size:
2371 806 537 504 198 81
SacI G_AGCT'C
Cuts at: 0 858 1929 2775 4497
Size: 858 1071 846 1722
Fragments arranged by size:
1722 1071 858 846
SanDI GG'GwC_CC
Cuts at: 0 2249 2788 2835 2907 3670 4497
Size: 2249 539 47 72 763 827
Fragments arranged by size:
2249 827 763 539 72 47
SbfI CC_TGCA'GG
Cuts at: 0 3228 4497
Size: 3228 1269
SexAI A'CCwGG_T
Cuts at: 0 489 507 723 948 1119 2073 2271 2451
Size: 489 18 216 225 171 954 198 180
Cuts at: 2451 2928 3333 4042 4497
Size: 477 405 709 455
Fragments arranged by size:
954 709 489 477 455 405 225 216
198 180 171 18
SfcI C'TryA_G
Cuts at: 0 704 1172 1640 2720 3125 3224 3323 4497
Size: 704 468 468 1080 405 99 99 1174
Fragments arranged by size:
1174 1080 704 468 468 405 99 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1850 3029 3579 3659 4497
Size: 1850 1179 550 80 838
Fragments arranged by size:
1850 1179 838 550 80
SmaI CCC'GGG
Cuts at: 0 1833 2488 2805 4497
Size: 1833 655 317 1692
Fragments arranged by size:
1833 1692 655 317
SmlI C'TyrA_G
Cuts at: 0 558 1622 2459 2504 4497
Size: 558 1064 837 45 1993
Fragments arranged by size:
1993 1064 837 558 45
SphI G_CATG'C
Cuts at: 0 2991 3018 3732 4497
Size: 2991 27 714 765
Fragments arranged by size:
2991 765 714 27
SspI AAT'ATT
Cuts at: 0 4314 4497
Size: 4314 183
StyI C'CwwG_G
Cuts at: 0 658 956 992 2324 2703 2793 3369 3948
Size: 658 298 36 1332 379 90 576 579
Cuts at: 3948 4497
Size: 549
Fragments arranged by size:
1332 658 579 576 549 379 298 90
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 557 976 1759 3262 3419 3887 4497
Size: 557 419 783 1503 157 468 610
Fragments arranged by size:
1503 783 610 557 468 419 157
TatI w'GTAC_w
Cuts at: 0 140 4497
Size: 140 4357
Tth111I GACn'n_nGTC
Cuts at: 0 2210 4497
Size: 2210 2287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 981 999 2710 3376 4040 4497
Size: 981 18 1711 666 664 457
Fragments arranged by size:
1711 981 666 664 457 18
XhoI C'TCGA_G
Cuts at: 0 2459 2504 4497
Size: 2459 45 1993
Fragments arranged by size:
2459 1993 45
XmaI C'CCGG_G
Cuts at: 0 1831 2486 2803 4497
Size: 1831 655 317 1694
Fragments arranged by size:
1831 1694 655 317
ZraI GAC'GTC
Cuts at: 0 1684 4497
Size: 1684 2813
Enzymes that do cut:
AatII Acc65I AcuI AflIII AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BsrFI BssSI BstAPI BstEII BstYI Bsu36I BtgI
BtsI DraIII DrdI EaeI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI Hin4I HincII HindIII
KpnI MfeI MmeI MscI MslI MspA1I NcoI NspI
PasI PciI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuII SacI SanDI SbfI SexAI SfcI
SfiI SmaI SmlI SphI SspI StyI TaqII TatI
Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AhdI AleI
AscI AseI AsiSI BaeI BbeI BbvCI BfrBI BmgBI
BmtI Bpu10I BsaBI BsiWI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BstBI BstXI BstZ17I BtgZI ClaI CspCI
DraI EagI EarI FseI FspAI HaeII HpaI KasI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PmeI PpiI PsiI PvuI RsrII
SacII SalI SapI ScaI SfoI SgfI SgrAI SnaBI
SpeI SrfI StuI SwaI TstI XbaI XmnI