Restriction map for
pF1KB3587
(Linear) (Six-base) MAPSORT of: KIBB3587.seq Check: 1670 from: 1 to: 1464
KIBB3587.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 514 1178 1464
Size: 514 664 286
Fragments arranged by size:
664 514 286
Acc65I G'GTAC_C
Cuts at: 0 434 1464
Size: 434 1030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 1464
Size: 324 1140
AflII C'TTAA_G
Cuts at: 0 733 1464
Size: 733 731
AflIII A'CryG_T
Cuts at: 0 1064 1464
Size: 1064 400
AlwNI CAG_nnn'CTG
Cuts at: 0 292 1314 1464
Size: 292 1022 150
Fragments arranged by size:
1022 292 150
ApaI G_GGCC'C
Cuts at: 0 23 953 1222 1464
Size: 23 930 269 242
Fragments arranged by size:
930 269 242 23
ApoI r'AATT_y
Cuts at: 0 1099 1464
Size: 1099 365
AvaI C'yCGr_G
Cuts at: 0 129 1174 1464
Size: 129 1045 290
Fragments arranged by size:
1045 290 129
BamHI G'GATC_C
Cuts at: 0 862 1464
Size: 862 602
BanI G'GyrC_C
Cuts at: 0 418 434 1329 1464
Size: 418 16 895 135
Fragments arranged by size:
895 418 135 16
BanII G_rGCy'C
Cuts at: 0 23 953 1145 1222 1464
Size: 23 930 192 77 242
Fragments arranged by size:
930 242 192 77 23
BbeI G_GCGC'C
Cuts at: 0 1333 1464
Size: 1333 131
BbsI GAAGACnn'nnnn_
Cuts at: 0 180 1464
Size: 180 1284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1083 1117 1464
Size: 1083 34 347
Fragments arranged by size:
1083 347 34
BclI T'GATC_A
Cuts at: 0 573 824 1464
Size: 573 251 640
Fragments arranged by size:
640 573 251
BglI GCCn_nnn'nGGC
Cuts at: 0 1344 1464
Size: 1344 120
BlpI GC'TnA_GC
Cuts at: 0 1453 1464
Size: 1453 11
Bme1580I G_kGCm'C
Cuts at: 0 23 953 1195 1222 1464
Size: 23 930 242 27 242
Fragments arranged by size:
930 242 242 27 23
BmgBI CAC'GTC
Cuts at: 0 693 1275 1464
Size: 693 582 189
Fragments arranged by size:
693 582 189
BmrI ACTGGGnnnn_n'
Cuts at: 0 1225 1464
Size: 1225 239
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1350 1464
Size: 1350 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1464
Size: 778 686
BsaAI yAC'GTr
Cuts at: 0 1392 1464
Size: 1392 72
BsaHI Gr'CG_yC
Cuts at: 0 185 1318 1330 1464
Size: 185 1133 12 134
Fragments arranged by size:
1133 185 134 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 49 79 1464
Size: 49 30 1385
Fragments arranged by size:
1385 49 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 54 1464
Size: 54 1410
BseYI C'CCAG_C
Cuts at: 0 1144 1395 1464
Size: 1144 251 69
Fragments arranged by size:
1144 251 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 1464
Size: 1150 314
BsiHKAI G_wGCw'C
Cuts at: 0 1096 1298 1464
Size: 1096 202 166
Fragments arranged by size:
1096 202 166
BsmI GAATG_Cn'
Cuts at: 0 676 1085 1464
Size: 676 409 379
Fragments arranged by size:
676 409 379
Bsp1286I G_dGCh'C
Cuts at: 0 23 953 1096 1145 1195 1222 1298 1464
Size: 23 930 143 49 50 27 76 166
Fragments arranged by size:
930 166 143 76 50 49 27 23
BspHI T'CATG_A
Cuts at: 0 164 1464
Size: 164 1300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 514 1178 1464
Size: 514 664 286
Fragments arranged by size:
664 514 286
BsrDI GCAATG_nn'
Cuts at: 0 537 1464
Size: 537 927
BssSI C'ACGA_G
Cuts at: 0 362 1464
Size: 362 1102
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1199 1464
Size: 1199 265
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1350 1464
Size: 1350 114
BstYI r'GATC_y
Cuts at: 0 862 1464
Size: 862 602
BtgI C'CryG_G
Cuts at: 0 71 1464
Size: 71 1393
DraIII CAC_nnn'GTG
Cuts at: 0 1060 1464
Size: 1060 404
DrdI GACnn_nn'nnGTC
Cuts at: 0 352 1464
Size: 352 1112
EaeI y'GGCC_r
Cuts at: 0 474 1403 1464
Size: 474 929 61
Fragments arranged by size:
929 474 61
EarI CTCTTCn'nnn_
Cuts at: 0 235 972 1464
Size: 235 737 492
Fragments arranged by size:
737 492 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 778 1464
Size: 324 454 686
Fragments arranged by size:
686 454 324
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1255 1423 1464
Size: 1255 168 41
Fragments arranged by size:
1255 168 41
EcoO109I rG'GnC_Cy
Cuts at: 0 320 1464
Size: 320 1144
EcoRV GAT'ATC
Cuts at: 0 408 1464
Size: 408 1056
HaeII r_GCGC'y
Cuts at: 0 1333 1464
Size: 1333 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 971 1003 1464
Size: 971 32 461
Fragments arranged by size:
971 461 32
KasI G'GCGC_C
Cuts at: 0 1329 1464
Size: 1329 135
KpnI G_GTAC'C
Cuts at: 0 438 1464
Size: 438 1026
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 857 1464
Size: 857 607
MscI TGG'CCA
Cuts at: 0 476 1405 1464
Size: 476 929 59
Fragments arranged by size:
929 476 59
MslI CAynn'nnrTG
Cuts at: 0 1033 1390 1464
Size: 1033 357 74
Fragments arranged by size:
1033 357 74
NarI GG'CG_CC
Cuts at: 0 1330 1464
Size: 1330 134
NcoI C'CATG_G
Cuts at: 0 71 1464
Size: 71 1393
NspI r_CATG'y
Cuts at: 0 1068 1464
Size: 1068 396
PasI CC'CwG_GG
Cuts at: 0 587 1464
Size: 587 877
PciI A'CATG_T
Cuts at: 0 1064 1464
Size: 1064 400
PfoI T'CCnGG_A
Cuts at: 0 755 1464
Size: 755 709
PmlI CAC'GTG
Cuts at: 0 1392 1464
Size: 1392 72
PpuMI rG'GwC_Cy
Cuts at: 0 320 1464
Size: 320 1144
PshAI GACnn'nnGTC
Cuts at: 0 184 202 1464
Size: 184 18 1262
Fragments arranged by size:
1262 184 18
PspOMI G'GGCC_C
Cuts at: 0 19 949 1218 1464
Size: 19 930 269 246
Fragments arranged by size:
930 269 246 19
PstI C_TGCA'G
Cuts at: 0 288 1119 1136 1464
Size: 288 831 17 328
Fragments arranged by size:
831 328 288 17
SanDI GG'GwC_CC
Cuts at: 0 320 1464
Size: 320 1144
SfcI C'TryA_G
Cuts at: 0 284 618 903 1115 1132 1464
Size: 284 334 285 212 17 332
Fragments arranged by size:
334 332 285 284 212 17
SfoI GGC'GCC
Cuts at: 0 1331 1464
Size: 1331 133
SmaI CCC'GGG
Cuts at: 0 131 1176 1464
Size: 131 1045 288
Fragments arranged by size:
1045 288 131
SmlI C'TyrA_G
Cuts at: 0 733 1464
Size: 733 731
StyI C'CwwG_G
Cuts at: 0 71 1464
Size: 71 1393
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 70 703 1464
Size: 70 633 761
Fragments arranged by size:
761 633 70
TatI w'GTAC_w
Cuts at: 0 38 1464
Size: 38 1426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 49 81 1464
Size: 49 32 1383
Fragments arranged by size:
1383 49 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1212 1392 1464
Size: 1212 180 72
Fragments arranged by size:
1212 180 72
XmaI C'CCGG_G
Cuts at: 0 129 1174 1464
Size: 129 1045 290
Fragments arranged by size:
1045 290 129
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BcgI BclI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BssSI BstAPI BstXI BstYI BtgI
DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I KasI KpnI MmeI MscI MslI NarI
NcoI NspI PasI PciI PfoI PmlI PpuMI PshAI
PspOMI PstI SanDI SfcI SfoI SmaI SmlI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI
BciVI BfrBI BglII BmtI Bpu10I BpuEI BsaI BsaBI
BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI EagI EciI EcoICRI EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MspA1I
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PmeI PpiI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmnI ZraI