(Linear) (Six-base) MAPSORT of: KIBB3574.seq  Check: 2029  from: 1  to: 1296



KIBB3574.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     787    1296 
   Size:        787     509
 
AccI GT'mk_AC

Cuts at:      0     668    1296 
   Size:        668     628
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      55    1296 
   Size:         55    1241
 
AlwNI CAG_nnn'CTG

Cuts at:      0     535     903    1296 
   Size:        535     368     393

  Fragments arranged by size: 

                 535     393     368
 
ApoI r'AATT_y

Cuts at:      0      51     208     255    1296 
   Size:         51     157      47    1041

  Fragments arranged by size: 

                1041     157      51      47
 
AseI AT'TA_AT

Cuts at:      0     433    1296 
   Size:        433     863
 
BanII G_rGCy'C

Cuts at:      0     647     764    1296 
   Size:        647     117     532

  Fragments arranged by size: 

                 647     532     117
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      62     571    1296 
   Size:         62     509     725

  Fragments arranged by size: 

                 725     509      62
 
BbvCI CC'TCA_GC

Cuts at:      0     491    1296 
   Size:        491     805
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     950    1296 
   Size:        950     346
 
BfrBI ATG'CAT

Cuts at:      0     397    1120    1296 
   Size:        397     723     176

  Fragments arranged by size: 

                 723     397     176
 
BlpI GC'TnA_GC

Cuts at:      0     632    1296 
   Size:        632     664
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     102    1296 
   Size:        102    1194
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557    1296 
   Size:        557     739
 
Bpu10I CC'TnA_GC

Cuts at:      0     215     491    1296 
   Size:        215     276     805

  Fragments arranged by size: 

                 805     276     215
 
BsaAI yAC'GTr

Cuts at:      0    1014    1296 
   Size:       1014     282
 
BsaBI GATnn'nnATC

Cuts at:      0     619    1296 
   Size:        619     677
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      77    1296 
   Size:         77    1219
 
BseYI C'CCAG_C

Cuts at:      0     903    1022    1296 
   Size:        903     119     274

  Fragments arranged by size: 

                 903     274     119
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     878    1296 
   Size:        878     418
 
BsiHKAI G_wGCw'C

Cuts at:      0     368    1296 
   Size:        368     928
 
Bsp1286I G_dGCh'C

Cuts at:      0     368     647     764    1296 
   Size:        368     279     117     532

  Fragments arranged by size: 

                 532     368     279     117
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     532     787    1296 
   Size:        532     255     509

  Fragments arranged by size: 

                 532     509     255
 
BsrDI GCAATG_nn'

Cuts at:      0    1268    1296 
   Size:       1268      28
 
BssSI C'ACGA_G

Cuts at:      0     367    1296 
   Size:        367     929
 
BstBI TT'CG_AA

Cuts at:      0     259     794    1296 
   Size:        259     535     502

  Fragments arranged by size: 

                 535     502     259
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     825    1026    1296 
   Size:        825     201     270

  Fragments arranged by size: 

                 825     270     201
 
BstYI r'GATC_y

Cuts at:      0     419    1296 
   Size:        419     877
 
BtgI C'CryG_G

Cuts at:      0     245    1296 
   Size:        245    1051
 
BtsI GCAGTG_nn'

Cuts at:      0      20     633    1296 
   Size:         20     613     663

  Fragments arranged by size: 

                 663     613      20
 
ClaI AT'CG_AT

Cuts at:      0    1283    1296 
   Size:       1283      13
 
DraIII CAC_nnn'GTG

Cuts at:      0    1005    1296 
   Size:       1005     291
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      66    1296 
   Size:         66    1230
 
EaeI y'GGCC_r

Cuts at:      0    1016    1106    1296 
   Size:       1016      90     190

  Fragments arranged by size: 

                1016     190      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557    1296 
   Size:        557     739
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     529    1296 
   Size:        529     767
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6     990    1296 
   Size:          6     984     306

  Fragments arranged by size: 

                 984     306       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      72     104    1296 
   Size:         72      32    1192

  Fragments arranged by size: 

                1192      72      32
 
HincII GTy'rAC

Cuts at:      0     321     788    1296 
   Size:        321     467     508

  Fragments arranged by size: 

                 508     467     321
 
HpaI GTT'AAC

Cuts at:      0     788    1296 
   Size:        788     508
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1020    1274    1296 
   Size:       1020     254      22

  Fragments arranged by size: 

                1020     254      22
 
MscI TGG'CCA

Cuts at:      0    1018    1108    1296 
   Size:       1018      90     188

  Fragments arranged by size: 

                1018     188      90
 
MslI CAynn'nnrTG

Cuts at:      0    1283    1296 
   Size:       1283      13
 
MspA1I CmG'CkG

Cuts at:      0    1026    1296 
   Size:       1026     270
 
NcoI C'CATG_G

Cuts at:      0     245    1296 
   Size:        245    1051
 
NsiI A_TGCA'T

Cuts at:      0     399    1122    1296 
   Size:        399     723     174

  Fragments arranged by size: 

                 723     399     174
 
NspI r_CATG'y

Cuts at:      0     640    1296 
   Size:        640     656
 
PacI TTA_AT'TAA

Cuts at:      0     437    1296 
   Size:        437     859
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     331    1296 
   Size:        331     965
 
PmlI CAC'GTG

Cuts at:      0    1014    1296 
   Size:       1014     282
 
PvuII CAG'CTG

Cuts at:      0    1026    1296 
   Size:       1026     270
 
SfcI C'TryA_G

Cuts at:      0     500    1296 
   Size:        500     796
 
SphI G_CATG'C

Cuts at:      0     640    1296 
   Size:        640     656
 
StyI C'CwwG_G

Cuts at:      0     245    1296 
   Size:        245    1051
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1036    1296 
   Size:       1036     260
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     612     644    1296 
   Size:        612      32     652

  Fragments arranged by size: 

                 652     612      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1207    1296 
   Size:       1207      89
 

 Enzymes that do cut:

     AarI     AccI     AlfI    AlwNI     ApoI     AseI    BanII     BbsI
    BbvCI    BciVI    BfrBI     BlpI     BmrI     BpmI   Bpu10I    BsaAI
    BsaBI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI
    BssSI    BstBI    BstXI    BstYI     BtgI     BtsI     ClaI   DraIII
     DrdI     EaeI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII     HpaI
     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI     PacI
    PflMI     PmlI    PvuII     SfcI     SphI     StyI    TaqII     TstI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI     BclI     BglI
    BglII Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII   BstAPI   BstEII  BstZ17I   Bsu36I
    BtgZI    CspCI     DraI     EagI     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     PasI     PciI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI