Restriction map for
pF1KB3547
(Linear) (Six-base) MAPSORT of: KIBB3547.seq Check: 6745 from: 1 to: 3273
KIBB3547.orf
With 182 enzymes: SgfI *
April 12, 2016 09:51 ..
AccI GT'mk_AC
Cuts at: 0 202 368 3273
Size: 202 166 2905
Fragments arranged by size:
2905 202 166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1564 1927 3273
Size: 1564 363 1346
Fragments arranged by size:
1564 1346 363
AflIII A'CryG_T
Cuts at: 0 1146 3031 3273
Size: 1146 1885 242
Fragments arranged by size:
1885 1146 242
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 61 93 3273
Size: 61 32 3180
Fragments arranged by size:
3180 61 32
ApoI r'AATT_y
Cuts at: 0 1235 1996 2007 2418 2433 3273
Size: 1235 761 11 411 15 840
Fragments arranged by size:
1235 840 761 411 15 11
AseI AT'TA_AT
Cuts at: 0 2069 3273
Size: 2069 1204
BanI G'GyrC_C
Cuts at: 0 2320 3273
Size: 2320 953
BanII G_rGCy'C
Cuts at: 0 526 1107 2681 3273
Size: 526 581 1574 592
Fragments arranged by size:
1574 592 581 526
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 602 726 1299 1389 3273
Size: 131 471 124 573 90 1884
Fragments arranged by size:
1884 573 471 131 124 90
BfrBI ATG'CAT
Cuts at: 0 1357 3273
Size: 1357 1916
BglII A'GATC_T
Cuts at: 0 277 3273
Size: 277 2996
BlpI GC'TnA_GC
Cuts at: 0 1322 2715 3273
Size: 1322 1393 558
Fragments arranged by size:
1393 1322 558
Bme1580I G_kGCm'C
Cuts at: 0 1522 3273
Size: 1522 1751
BmrI ACTGGGnnnn_n'
Cuts at: 0 1375 2895 3273
Size: 1375 1520 378
Fragments arranged by size:
1520 1375 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2142 2228 3273
Size: 2142 86 1045
Fragments arranged by size:
2142 1045 86
Bpu10I CC'TnA_GC
Cuts at: 0 893 3273
Size: 893 2380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1709 2823 2848 3273
Size: 1709 1114 25 425
Fragments arranged by size:
1709 1114 425 25
BsaAI yAC'GTr
Cuts at: 0 1147 3273
Size: 1147 2126
BsaBI GATnn'nnATC
Cuts at: 0 392 3273
Size: 392 2881
BsaWI w'CCGG_w
Cuts at: 0 1392 3273
Size: 1392 1881
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 1457 1487 3273
Size: 61 30 1366 30 1786
Fragments arranged by size:
1786 1366 61 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 1106 3273
Size: 85 1021 2167
Fragments arranged by size:
2167 1021 85
BseYI C'CCAG_C
Cuts at: 0 2602 3273
Size: 2602 671
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2672 3273
Size: 2672 601
BsiEI CG_ry'CG
Cuts at: 0 89 3273
Size: 89 3184
Bsp1286I G_dGCh'C
Cuts at: 0 526 1107 1522 2681 3273
Size: 526 581 415 1159 592
Fragments arranged by size:
1159 592 581 526 415
BspHI T'CATG_A
Cuts at: 0 2553 3273
Size: 2553 720
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 606 2397 3273
Size: 606 1791 876
Fragments arranged by size:
1791 876 606
BsrDI GCAATG_nn'
Cuts at: 0 363 894 1971 3273
Size: 363 531 1077 1302
Fragments arranged by size:
1302 1077 531 363
BsrFI r'CCGG_y
Cuts at: 0 1408 3273
Size: 1408 1865
BstBI TT'CG_AA
Cuts at: 0 1665 3273
Size: 1665 1608
BstYI r'GATC_y
Cuts at: 0 277 2457 3273
Size: 277 2180 816
Fragments arranged by size:
2180 816 277
BstZ17I GTA'TAC
Cuts at: 0 203 3273
Size: 203 3070
BtgI C'CryG_G
Cuts at: 0 664 724 3273
Size: 664 60 2549
Fragments arranged by size:
2549 664 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2695 3273
Size: 2695 578
BtsI GCAGTG_nn'
Cuts at: 0 633 2288 2765 3273
Size: 633 1655 477 508
Fragments arranged by size:
1655 633 508 477
ClaI AT'CG_AT
Cuts at: 0 1544 3273
Size: 1544 1729
DraI TTT'AAA
Cuts at: 0 2006 3273
Size: 2006 1267
EaeI y'GGCC_r
Cuts at: 0 86 1410 3273
Size: 86 1324 1863
Fragments arranged by size:
1863 1324 86
EagI C'GGCC_G
Cuts at: 0 86 3273
Size: 86 3187
EarI CTCTTCn'nnn_
Cuts at: 0 1091 1394 1844 3273
Size: 1091 303 450 1429
Fragments arranged by size:
1429 1091 450 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1564 1927 2142 2228 3273
Size: 1564 363 215 86 1045
Fragments arranged by size:
1564 1045 363 215 86
EcoO109I rG'GnC_Cy
Cuts at: 0 1189 3127 3273
Size: 1189 1938 146
Fragments arranged by size:
1938 1189 146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 145 778 1023 1449 2395 3273
Size: 145 633 245 426 946 878
Fragments arranged by size:
946 878 633 426 245 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1845 1877 2535 2567 2650 2682 2911 2943
Size: 1845 32 658 32 83 32 229 32
Cuts at: 2943 3273
Size: 330
Fragments arranged by size:
1845 658 330 229 83 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1171 3273
Size: 1171 2102
HindIII A'AGCT_T
Cuts at: 0 2983 3273
Size: 2983 290
MfeI C'AATT_G
Cuts at: 0 530 3273
Size: 530 2743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 2379 3273
Size: 47 2332 894
Fragments arranged by size:
2332 894 47
MslI CAynn'nnrTG
Cuts at: 0 609 1178 1764 3036 3273
Size: 609 569 586 1272 237
Fragments arranged by size:
1272 609 586 569 237
MspA1I CmG'CkG
Cuts at: 0 231 1029 1446 3273
Size: 231 798 417 1827
Fragments arranged by size:
1827 798 417 231
NcoI C'CATG_G
Cuts at: 0 664 724 3273
Size: 664 60 2549
Fragments arranged by size:
2549 664 60
NdeI CA'TA_TG
Cuts at: 0 2238 2721 3273
Size: 2238 483 552
Fragments arranged by size:
2238 552 483
NruI TCG'CGA
Cuts at: 0 590 3273
Size: 590 2683
NsiI A_TGCA'T
Cuts at: 0 1359 3273
Size: 1359 1914
NspI r_CATG'y
Cuts at: 0 762 3035 3273
Size: 762 2273 238
Fragments arranged by size:
2273 762 238
PciI A'CATG_T
Cuts at: 0 3031 3273
Size: 3031 242
PfoI T'CCnGG_A
Cuts at: 0 2422 3273
Size: 2422 851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 3273
Size: 61 32 3180
Fragments arranged by size:
3180 61 32
PpuMI rG'GwC_Cy
Cuts at: 0 1189 3273
Size: 1189 2084
PshAI GACnn'nnGTC
Cuts at: 0 1774 3273
Size: 1774 1499
PsiI TTA'TAA
Cuts at: 0 998 1040 3273
Size: 998 42 2233
Fragments arranged by size:
2233 998 42
PstI C_TGCA'G
Cuts at: 0 2394 3273
Size: 2394 879
PvuII CAG'CTG
Cuts at: 0 231 1029 1446 3273
Size: 231 798 417 1827
Fragments arranged by size:
1827 798 417 231
SexAI A'CCwGG_T
Cuts at: 0 2316 2618 3273
Size: 2316 302 655
Fragments arranged by size:
2316 655 302
SfcI C'TryA_G
Cuts at: 0 1818 2354 2390 2788 3273
Size: 1818 536 36 398 485
Fragments arranged by size:
1818 536 485 398 36
SmlI C'TyrA_G
Cuts at: 0 1724 2802 2827 3273
Size: 1724 1078 25 446
Fragments arranged by size:
1724 1078 446 25
SspI AAT'ATT
Cuts at: 0 1276 3005 3273
Size: 1276 1729 268
Fragments arranged by size:
1729 1276 268
StyI C'CwwG_G
Cuts at: 0 664 724 735 1802 3273
Size: 664 60 11 1067 1471
Fragments arranged by size:
1471 1067 664 60 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 96 3273
Size: 96 3177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1194 1226 1457 1489 3273
Size: 1194 32 231 32 1784
Fragments arranged by size:
1784 1194 231 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 130 3273
Size: 130 3143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 662 1450 3273
Size: 662 788 1823
Fragments arranged by size:
1823 788 662
XmnI GAAnn'nnTTC
Cuts at: 0 2958 3273
Size: 2958 315
Enzymes that do cut:
AccI AcuI AflIII AloI ApoI AseI BanI BanII
BbsI BfrBI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiEI Bsp1286I BspHI BspMI BsrDI BsrFI BstBI BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraI EaeI EagI
EarI Eco57MI EcoO109I FalI Hin4I HincII HindIII MfeI
MmeI MslI MspA1I NcoI NdeI NruI NsiI NspI
PciI PfoI PpiI PpuMI PshAI PsiI PstI PvuII
SexAI SfcI SmlI SspI StyI TaqII TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BglI BmgBI BmtI BplI BsaI BsaHI BsiHKAI BsiWI
BsmI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstAPI
BstEII BstXI Bsu36I CspCI DraIII DrdI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MluI MscI NaeI NarI NgoMIV NheI
NotI PacI PasI PflMI PmeI PmlI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TatI XbaI XhoI
XmaI ZraI