Restriction map for
pF1KB3507
(Linear) (Six-base) MAPSORT of: KIBB3507.seq Check: 5300 from: 1 to: 1119
KIBB3507.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 33 1119
Size: 33 1086
AatII G_ACGT'C
Cuts at: 0 897 1119
Size: 897 222
AccI GT'mk_AC
Cuts at: 0 409 1119
Size: 409 710
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 820 1119
Size: 750 70 299
Fragments arranged by size:
750 299 70
AflIII A'CryG_T
Cuts at: 0 50 227 1072 1119
Size: 50 177 845 47
Fragments arranged by size:
845 177 50 47
AleI CACnn'nnGTG
Cuts at: 0 854 1119
Size: 854 265
ApaI G_GGCC'C
Cuts at: 0 470 1119
Size: 470 649
ApoI r'AATT_y
Cuts at: 0 180 607 1119
Size: 180 427 512
Fragments arranged by size:
512 427 180
BanI G'GyrC_C
Cuts at: 0 431 839 1119
Size: 431 408 280
Fragments arranged by size:
431 408 280
BanII G_rGCy'C
Cuts at: 0 152 470 1103 1119
Size: 152 318 633 16
Fragments arranged by size:
633 318 152 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 695 1069 1119
Size: 695 374 50
Fragments arranged by size:
695 374 50
BbvCI CC'TCA_GC
Cuts at: 0 9 1119
Size: 9 1110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 504 538 919 953 1119
Size: 504 34 381 34 166
Fragments arranged by size:
504 381 166 34 34
Bme1580I G_kGCm'C
Cuts at: 0 434 470 1119
Size: 434 36 649
Fragments arranged by size:
649 434 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 594 1119
Size: 594 525
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 667 1119
Size: 667 452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 177 578 1119
Size: 128 49 401 541
Fragments arranged by size:
541 401 128 49
Bpu10I CC'TnA_GC
Cuts at: 0 9 719 1119
Size: 9 710 400
Fragments arranged by size:
710 400 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 322 1119
Size: 322 797
BsaAI yAC'GTr
Cuts at: 0 1073 1119
Size: 1073 46
BsaHI Gr'CG_yC
Cuts at: 0 894 1119
Size: 894 225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 1119
Size: 391 30 698
Fragments arranged by size:
698 391 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 802 826 1119
Size: 802 24 293
Fragments arranged by size:
802 293 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1119
Size: 767 352
BsmI GAATG_Cn'
Cuts at: 0 971 1119
Size: 971 148
Bsp1286I G_dGCh'C
Cuts at: 0 152 434 470 1103 1119
Size: 152 282 36 633 16
Fragments arranged by size:
633 282 152 36 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 33 1119
Size: 33 1086
BsrFI r'CCGG_y
Cuts at: 0 1090 1119
Size: 1090 29
BsrGI T'GTAC_A
Cuts at: 0 47 1119
Size: 47 1072
BssSI C'ACGA_G
Cuts at: 0 235 1119
Size: 235 884
BstXI CCAn_nnnn'nTGG
Cuts at: 0 159 1119
Size: 159 960
BstZ17I GTA'TAC
Cuts at: 0 410 1119
Size: 410 709
Bsu36I CC'TnA_GG
Cuts at: 0 62 1119
Size: 62 1057
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1109 1119
Size: 1109 10
ClaI AT'CG_AT
Cuts at: 0 98 1119
Size: 98 1021
EaeI y'GGCC_r
Cuts at: 0 987 1119
Size: 987 132
EarI CTCTTCn'nnn_
Cuts at: 0 158 1119
Size: 158 961
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 177 578 750 820 1119
Size: 128 49 401 172 70 299
Fragments arranged by size:
401 299 172 128 70 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 61 513 626 1119
Size: 61 452 113 493
Fragments arranged by size:
493 452 113 61
EcoO109I rG'GnC_Cy
Cuts at: 0 258 273 466 467 1119
Size: 258 15 193 1 652
Fragments arranged by size:
652 258 193 15 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1023 1119
Size: 1023 96
HaeII r_GCGC'y
Cuts at: 0 962 1119
Size: 962 157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 476 944 1119
Size: 242 234 468 175
Fragments arranged by size:
468 242 234 175
MscI TGG'CCA
Cuts at: 0 989 1119
Size: 989 130
MslI CAynn'nnrTG
Cuts at: 0 854 1119
Size: 854 265
NspI r_CATG'y
Cuts at: 0 54 231 1119
Size: 54 177 888
Fragments arranged by size:
888 177 54
PasI CC'CwG_GG
Cuts at: 0 463 471 1119
Size: 463 8 648
Fragments arranged by size:
648 463 8
PciI A'CATG_T
Cuts at: 0 50 227 1119
Size: 50 177 892
Fragments arranged by size:
892 177 50
PmlI CAC'GTG
Cuts at: 0 1073 1119
Size: 1073 46
PpuMI rG'GwC_Cy
Cuts at: 0 258 273 1119
Size: 258 15 846
Fragments arranged by size:
846 258 15
PspOMI G'GGCC_C
Cuts at: 0 466 1119
Size: 466 653
PstI C_TGCA'G
Cuts at: 0 327 1119
Size: 327 792
SanDI GG'GwC_CC
Cuts at: 0 258 1119
Size: 258 861
ScaI AGT'ACT
Cuts at: 0 198 1119
Size: 198 921
SfcI C'TryA_G
Cuts at: 0 323 1119
Size: 323 796
StuI AGG'CCT
Cuts at: 0 321 878 1119
Size: 321 557 241
Fragments arranged by size:
557 321 241
StyI C'CwwG_G
Cuts at: 0 773 869 1119
Size: 773 96 250
Fragments arranged by size:
773 250 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 645 1119
Size: 645 474
TatI w'GTAC_w
Cuts at: 0 47 196 308 803 1109 1119
Size: 47 149 112 495 306 10
Fragments arranged by size:
495 306 149 112 47 10
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 876 1119
Size: 876 243
ZraI GAC'GTC
Cuts at: 0 895 1119
Size: 895 224
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI ApaI ApoI
BanI BanII BbsI BbvCI BcgI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BsgI
BsmI Bsp1286I BspMI BsrFI BsrGI BssSI BstXI BstZ17I
Bsu36I BtgZI ClaI EaeI EarI Eco57MI EcoNI EcoO109I
FalI HaeII MmeI MscI MslI NspI PasI PciI
PmlI PpuMI PspOMI PstI SanDI ScaI SfcI StuI
StyI TaqII TatI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BpuEI BsaBI BsaWI BseYI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BssHII BstAPI
BstBI BstEII BstYI BtgI BtsI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI