(Linear) (Six-base) MAPSORT of: KIBB3484.seq  Check: 557  from: 1  to: 633



KIBB3484.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     633 
   Size:        364     269
 
AhdI GACnn_n'nnGTC

Cuts at:      0     371     633 
   Size:        371     262
 
ApoI r'AATT_y

Cuts at:      0      23     633 
   Size:         23     610
 
BanII G_rGCy'C

Cuts at:      0      39     633 
   Size:         39     594
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     217     633 
   Size:        217     416
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     406     493     633 
   Size:        406      87     140

  Fragments arranged by size: 

                 406     140      87
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     633 
   Size:        455     178
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      87     633 
   Size:         87     546
 
BsiHKAI G_wGCw'C

Cuts at:      0      39     633 
   Size:         39     594
 
Bsp1286I G_dGCh'C

Cuts at:      0      39     633 
   Size:         39     594
 
BsrDI GCAATG_nn'

Cuts at:      0     342     528     633 
   Size:        342     186     105

  Fragments arranged by size: 

                 342     186     105
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     219     254     633 
   Size:        219      35     379

  Fragments arranged by size: 

                 379     219      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     452     633 
   Size:        452     181
 
EcoICRI GAG'CTC

Cuts at:      0      37     633 
   Size:         37     596
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     633 
   Size:        364     269
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     357     389     519     551     633 
   Size:         27     330      32     130      32      82

  Fragments arranged by size: 

                 330     130      82      32      32      27
 
MslI CAynn'nnrTG

Cuts at:      0     127     633 
   Size:        127     506
 
PsiI TTA'TAA

Cuts at:      0     296     633 
   Size:        296     337
 
SacI G_AGCT'C

Cuts at:      0      39     633 
   Size:         39     594
 
SmlI C'TyrA_G

Cuts at:      0     470     633 
   Size:        470     163
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     129     633 
   Size:        129     504
 
XmnI GAAnn'nnTTC

Cuts at:      0      23     633 
   Size:         23     610
 

 Enzymes that do cut:

     AcuI     AhdI     ApoI    BanII     BbsI    BciVI    BpuEI    BseRI
  BsiHKAI Bsp1286I    BsrDI    CspCI     EarI  EcoICRI  Eco57MI    Hin4I
     MslI     PsiI     SacI     SmlI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI