Restriction map for
pF1KB3477
(Linear) (Six-base) MAPSORT of: KIBB3477.seq Check: 9576 from: 1 to: 603
KIBB3477.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 0 269 603
Size: 269 334
AfeI AGC'GCT
Cuts at: 0 205 603
Size: 205 398
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 463 495 603
Size: 463 32 108
Fragments arranged by size:
463 108 32
ApoI r'AATT_y
Cuts at: 0 409 603
Size: 409 194
AvaI C'yCGr_G
Cuts at: 0 5 603
Size: 5 598
BglII A'GATC_T
Cuts at: 0 179 603
Size: 179 424
Bpu10I CC'TnA_GC
Cuts at: 0 374 603
Size: 374 229
BsaI GGTCTCn'nnnn_
Cuts at: 0 436 603
Size: 436 167
BsaAI yAC'GTr
Cuts at: 0 227 603
Size: 227 376
BsaBI GATnn'nnATC
Cuts at: 0 427 603
Size: 427 176
BsaHI Gr'CG_yC
Cuts at: 0 266 603
Size: 266 337
BsaWI w'CCGG_w
Cuts at: 0 140 603
Size: 140 463
BsiEI CG_ry'CG
Cuts at: 0 122 603
Size: 122 481
BsmBI CGTCTCn'nnnn_
Cuts at: 0 385 603
Size: 385 218
BspEI T'CCGG_A
Cuts at: 0 140 603
Size: 140 463
BsrBI CCG'CTC
Cuts at: 0 378 603
Size: 378 225
BstYI r'GATC_y
Cuts at: 0 137 179 603
Size: 137 42 424
Fragments arranged by size:
424 137 42
BtgI C'CryG_G
Cuts at: 0 146 243 603
Size: 146 97 360
Fragments arranged by size:
360 146 97
EarI CTCTTCn'nnn_
Cuts at: 0 28 467 603
Size: 28 439 136
Fragments arranged by size:
439 136 28
EcoO109I rG'GnC_Cy
Cuts at: 0 239 603
Size: 239 364
HaeII r_GCGC'y
Cuts at: 0 207 452 603
Size: 207 245 151
Fragments arranged by size:
245 207 151
HincII GTy'rAC
Cuts at: 0 291 465 603
Size: 291 174 138
Fragments arranged by size:
291 174 138
MspA1I CmG'CkG
Cuts at: 0 50 193 603
Size: 50 143 410
Fragments arranged by size:
410 143 50
PpuMI rG'GwC_Cy
Cuts at: 0 239 603
Size: 239 364
PstI C_TGCA'G
Cuts at: 0 179 603
Size: 179 424
PvuI CG_AT'CG
Cuts at: 0 122 603
Size: 122 481
RsrII CG'GwC_CG
Cuts at: 0 143 603
Size: 143 460
SanDI GG'GwC_CC
Cuts at: 0 239 603
Size: 239 364
SfcI C'TryA_G
Cuts at: 0 175 603
Size: 175 428
SmaI CCC'GGG
Cuts at: 0 7 603
Size: 7 596
SspI AAT'ATT
Cuts at: 0 347 603
Size: 347 256
StyI C'CwwG_G
Cuts at: 0 452 603
Size: 452 151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 123 603
Size: 123 480
XmaI C'CCGG_G
Cuts at: 0 5 603
Size: 5 598
ZraI GAC'GTC
Cuts at: 0 267 603
Size: 267 336
Enzymes that do cut:
AatII AfeI AloI ApoI AvaI BglII Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsiEI BsmBI BspEI BsrBI
BstYI BtgI EarI EcoO109I HaeII HincII MspA1I PpuMI
PstI PvuI RsrII SanDI SfcI SmaI SspI StyI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaXI BseRI
BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmnI