Restriction map for
pF1KB3382
(Linear) (Six-base) MAPSORT of: KIBB3382.seq Check: 3952 from: 1 to: 1263
KIBB3382.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 489 1263
Size: 489 774
AflIII A'CryG_T
Cuts at: 0 779 1263
Size: 779 484
AhdI GACnn_n'nnGTC
Cuts at: 0 670 1263
Size: 670 593
AleI CACnn'nnGTG
Cuts at: 0 310 1263
Size: 310 953
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 844 876 1263
Size: 844 32 387
Fragments arranged by size:
844 387 32
ApoI r'AATT_y
Cuts at: 0 870 1263
Size: 870 393
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 355 388 1263
Size: 355 33 875
Fragments arranged by size:
875 355 33
BanI G'GyrC_C
Cuts at: 0 289 489 742 1114 1263
Size: 289 200 253 372 149
Fragments arranged by size:
372 289 253 200 149
BanII G_rGCy'C
Cuts at: 0 322 433 473 1162 1263
Size: 322 111 40 689 101
Fragments arranged by size:
689 322 111 101 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 411 1263
Size: 411 852
BbvCI CC'TCA_GC
Cuts at: 0 215 995 1263
Size: 215 780 268
Fragments arranged by size:
780 268 215
BciVI GTATCCnnnnn_n'
Cuts at: 0 198 1263
Size: 198 1065
BglI GCCn_nnn'nGGC
Cuts at: 0 611 653 1263
Size: 611 42 610
Fragments arranged by size:
611 610 42
Bme1580I G_kGCm'C
Cuts at: 0 294 829 1117 1135 1263
Size: 294 535 288 18 128
Fragments arranged by size:
535 294 288 128 18
BmgBI CAC'GTC
Cuts at: 0 132 1263
Size: 132 1131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1008 1176 1207 1263
Size: 1008 168 31 56
Fragments arranged by size:
1008 168 56 31
Bpu10I CC'TnA_GC
Cuts at: 0 215 995 1263
Size: 215 780 268
Fragments arranged by size:
780 268 215
BsaI GGTCTCn'nnnn_
Cuts at: 0 78 1263
Size: 78 1185
BsaAI yAC'GTr
Cuts at: 0 717 780 1263
Size: 717 63 483
Fragments arranged by size:
717 483 63
BsaHI Gr'CG_yC
Cuts at: 0 1151 1263
Size: 1151 112
BsaWI w'CCGG_w
Cuts at: 0 195 1263
Size: 195 1068
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1012 1032 1263
Size: 1012 20 231
Fragments arranged by size:
1012 231 20
BseYI C'CCAG_C
Cuts at: 0 36 141 147 220 975 1128 1263
Size: 36 105 6 73 755 153 135
Fragments arranged by size:
755 153 135 105 73 36 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 517 523 1263
Size: 229 288 6 740
Fragments arranged by size:
740 288 229 6
Bsp1286I G_dGCh'C
Cuts at: 0 294 322 433 473 829 1117 1135 1162
Size: 294 28 111 40 356 288 18 27
Cuts at: 1162 1263
Size: 101
Fragments arranged by size:
356 294 288 111 101 40 28 27
18
BspEI T'CCGG_A
Cuts at: 0 195 1263
Size: 195 1068
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 489 1263
Size: 489 774
BsrDI GCAATG_nn'
Cuts at: 0 285 1263
Size: 285 978
BsrFI r'CCGG_y
Cuts at: 0 177 647 1263
Size: 177 470 616
Fragments arranged by size:
616 470 177
BstEII G'GTnAC_C
Cuts at: 0 571 1263
Size: 571 692
BstXI CCAn_nnnn'nTGG
Cuts at: 0 814 1263
Size: 814 449
Bsu36I CC'TnA_GG
Cuts at: 0 534 1263
Size: 534 729
BtgI C'CryG_G
Cuts at: 0 639 1263
Size: 639 624
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 624 659 1263
Size: 624 35 604
Fragments arranged by size:
624 604 35
DraIII CAC_nnn'GTG
Cuts at: 0 237 239 552 1263
Size: 237 2 313 711
Fragments arranged by size:
711 313 237 2
EaeI y'GGCC_r
Cuts at: 0 255 411 645 903 1263
Size: 255 156 234 258 360
Fragments arranged by size:
360 258 255 234 156
EarI CTCTTCn'nnn_
Cuts at: 0 475 997 1263
Size: 475 522 266
Fragments arranged by size:
522 475 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1008 1176 1207 1263
Size: 1008 168 31 56
Fragments arranged by size:
1008 168 56 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 985 1263
Size: 985 278
EcoO109I rG'GnC_Cy
Cuts at: 0 29 83 1058 1263
Size: 29 54 975 205
Fragments arranged by size:
975 205 54 29
EcoRI G'AATT_C
Cuts at: 0 870 1263
Size: 870 393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 656 688 1263
Size: 656 32 575
Fragments arranged by size:
656 575 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 824 1263
Size: 446 378 439
Fragments arranged by size:
446 439 378
MscI TGG'CCA
Cuts at: 0 257 905 1263
Size: 257 648 358
Fragments arranged by size:
648 358 257
MslI CAynn'nnrTG
Cuts at: 0 310 1263
Size: 310 953
MspA1I CmG'CkG
Cuts at: 0 141 507 565 1128 1185 1217 1263
Size: 141 366 58 563 57 32 46
Fragments arranged by size:
563 366 141 58 57 46 32
NaeI GCC'GGC
Cuts at: 0 179 649 1263
Size: 179 470 614
Fragments arranged by size:
614 470 179
NgoMIV G'CCGG_C
Cuts at: 0 177 647 1263
Size: 177 470 616
Fragments arranged by size:
616 470 177
NspI r_CATG'y
Cuts at: 0 231 1263
Size: 231 1032
PasI CC'CwG_GG
Cuts at: 0 294 334 1263
Size: 294 40 929
Fragments arranged by size:
929 294 40
PflMI CCAn_nnn'nTGG
Cuts at: 0 884 1263
Size: 884 379
PfoI T'CCnGG_A
Cuts at: 0 437 725 1263
Size: 437 288 538
Fragments arranged by size:
538 437 288
PmlI CAC'GTG
Cuts at: 0 717 780 1263
Size: 717 63 483
Fragments arranged by size:
717 483 63
PpuMI rG'GwC_Cy
Cuts at: 0 29 83 1263
Size: 29 54 1180
Fragments arranged by size:
1180 54 29
PvuII CAG'CTG
Cuts at: 0 141 507 565 1128 1185 1263
Size: 141 366 58 563 57 78
Fragments arranged by size:
563 366 141 78 58 57
SapI GCTCTTCn'nnn_
Cuts at: 0 475 997 1263
Size: 475 522 266
Fragments arranged by size:
522 475 266
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 611 653 1263
Size: 611 42 610
Fragments arranged by size:
611 610 42
SphI G_CATG'C
Cuts at: 0 231 1263
Size: 231 1032
StuI AGG'CCT
Cuts at: 0 656 1263
Size: 656 607
StyI C'CwwG_G
Cuts at: 0 584 981 1082 1263
Size: 584 397 101 181
Fragments arranged by size:
584 397 181 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 139 876 1263
Size: 21 118 737 387
Fragments arranged by size:
737 387 118 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 885 1125 1263
Size: 885 240 138
Fragments arranged by size:
885 240 138
Enzymes that do cut:
AarI AflIII AhdI AleI AloI ApoI BaeI BanI
BanII BbsI BbvCI BciVI BglI Bme1580I BmgBI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI
Bsp1286I BspEI BspMI BsrDI BsrFI BstEII BstXI Bsu36I
BtgI CspCI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PasI PflMI PfoI PmlI PpuMI PvuII SapI
SfiI SphI StuI StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BcgI BclI BfrBI BglII BlpI
BmrI BmtI BplI BpuEI BsaBI BsaXI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspHI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstYI BstZ17I BtgZI BtsI ClaI DraI
DrdI EagI EciI EcoICRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI